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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1902-1909.doi: 10.3724/SP.J.1006.2010.01902

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Genetic Diversity of Allotetraploid Cotton Based on SSR

GAO  Wei1,LIU  Fang2,LI  Shao-Hui2,WANG  Chun-Ying2,ZHANG  Xiang-Di2,WANG  Yu-Hong2,WANG  Kun-Bo12*   

  1. 1 College of Plant Science＆Technology, Huazhong Agricultural University, Wuhan 430070, China; 2 Cotton Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang 455000, China
  • Received:2010-03-04 Revised:2010-05-23 Online:2010-11-12 Published:2010-08-10
  • Contact: WANG Kun-Bo,E-mail:wkbcri@cricaas.com.cn;wkbcri@hotmail.com

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Abstract:  There are five allotetraploid cotton species including two cultivated varieties, G. hirsutum and G. barbadense, and three wild species, G. darwinii watt, G. mustelinum Miers ex Watt and G. tomentosum. Wild cotton contains not only wild species but also wild types of cultivated varieties, such as G. hirsutum L. races. Research on genetic diversity relationships among cultivated and wild cotton species is necessary for better understanding of cotton evolution and good use of wild resources. The aim of this study was to evaluate the genetic diversity of five allotetraploid cotton species and 42 wild types of upland cotton using SSR markers. A total of 95 from 1050 pairs of SSR primers were used to amplify the 60 accessions of germplasm, which could produce steady and clear polymorphic bands. Six hundred and sixty DNA fragments were scored among all materials, 584 polymorphic bands were obtained, accounting for 88.5% of the total bands. The allele number (NA) per SSR locus was 2–12, with the average of 6.1. UPGMA Cluster analysis based on SSR data showed that the genetic similarity (GS) coefficient among 42 accessions of germplasm ranged from 0.306 to 1.000, the mean genetic similarity (GS) coefficient was 0.493. For 95 primers, the polymorphism information content (PIC) was from 0.278 to 0.905. The Shannon-Weaver diversity index (H¢) ranged from 0.451 to 2.451, and effective number of alleles (Ne) varied from 1.385 to 10.490. The results showed that there is a wide genetic variation in wild types of upland cotton and allotetraploid cotton tested by SSR markers. The genetic relationship is nearest between upland cotton and G. hirsutum var. latifolium, very close Gossypium barbadense L. and G. darwinii watt, G. mustelinum Miers ex Watt and G. tomentosum L. Nuttall ex Seeman a bit close and farthest between upland cotton and others.

Key words: Wild races, Allotetraploid cotton species, Genetic diversity, SSR marker

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