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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1777-1785.doi: 10.3724/SP.J.1006.2010.01777

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Changes in the Pattern of Organization of Microtubules during Meiosis in Pollen Mother Cell (PMC) of Autotetraploid Rice

HE Jin-Hua,CHENG Xing-An**,CHEN Zhi-Xiong,GUO Hai-Bin,LIU Xiang-Dong*,LU Yong-Gen*   

  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding / South China Agricultural University, Guangzhou 510642, China
  • Received:2010-03-03 Revised:2010-05-26 Online:2010-10-12 Published:2010-08-04

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Abstract: Changes of microtubule distribution and chromosome behavior in autotetraploid rice during meiosis of PMC were detected in detail by using indirect-immunofluorescence laser scanning confocal microscopy. The result showed that microtubule distribution pattern in PMC of autotetraploid rice was similar to that of diploid rice during meiosis. But in comparison to diploid rice, different microtubule behaviors, such as longer circumferential microtubules, more spindle microtubules, were found in autotetraploid rice. At prophase I, microtubules were decreased a lot in number and distributed unevenly at leptotene. At zygotene, microtubules with no obvious polar distribution formed a diffuse network. At pachytene, nucleolus disintegration and distorted microtubules structure were found. At diplotene, no circumferential microtubule was found and microtubules depolymerization displayed in advance. At diakinesis, microtubules were decreased a lot in number. Perinuclear microtubules abnormally knitted. At MetaphaseI, many kinds of abnormalities appeared in spindle. At telophase I, phragmoplast was abnormal, and speckle microtubules were found in the cell. At telophaseII-tetra stage, two daughter cells connected, and microtubules randomly arranged in tri-fission. The results showed that microtubule abnormal reorganization pattern had a close relationship with abnormal chromosome behavior, which may act synergistically on pollen fertility and seed setting rate in autotetraploid rice.

Key words: Rice(Oryza sativa L.), Autotetraploid rice, Meiosis, Meiosis, Pollen, Microtubule

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