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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 177-183.doi: 10.3724/SP.J.1006.2013.00177

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of High Temperature Stress on Physiological Characteristics of Anther and Pollen Traits of Rice at Flowering Stage

ZHANG Gui-Lian1,2,ZHANG Shun-Tang2,XIAO Lang-Tao3,WU Xiao-Jin1,XIAO Ying-Hui2,CHEN Li-Yun2,*   

  1. 1,#  Post-doctoral Innovation Base of Agricultural Science and Technology Promotion Center of Longgang District , Shenzhen 518118, China; 2,#  College of Agronomy,Hunan Agricultural University, Changsha 410128, China; 3 Key Laboratory of Phytohormones Hunan Agricultural University, Changsha 410128, China
  • Received:2012-05-02 Revised:2012-09-05 Online:2013-01-12 Published:2012-11-14
  • Contact: 陈立云,E-mail: chenliyun996@163.com, Tel: 0731-84618757

Abstract:

To ascertain the mechanism affecting flower organ of rice by high temperature stress at flowering stage, we treated heat tolerant line 996 and heat-sensitive line 4628 with hightemperature (8:00–17:00, 37°C; 17:00–8:00, 30°C) and optimal temperature (8:00–17:00, 30°C; 17:00–8:00, 25°C) in the growth chambers. The antioxidant enzyme activities, membrane permeability and MDA content in anther and pollen characters of rice were studied. The results showed the superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbic acid-peroxidase (AsA-POD) activities in anther of rice increased obviously at early stage of high temperature stress, then decreased rapidly, and higher in heat tolerant line 996 than in line 4628, malonyldialdehyde (MDA) content and membrane permeability in heat-sensitive line 4628 were greater than those in heat tolerant line 996. The anther dehiscence coefficient, pollen germination rate and pollen grains number on the stigma significantly decreased, pollen diameter increased under high temperature stress. Anther dehiscence coefficient, pollen germination rate and pollen grains number on the stigma in heat tolerant line 996 were significantly higher than those in heat-sensitive line 4628 under high temperature stress. Under high temperature stress, higher antioxidant enzyme activities, better pollen grain pollination and germination character, as well as lower membrane permeability and MDA content of anther in rice could be the physiological basis of high-temperature tolerance.

Key words: Rice, High temperature at flowering stage, Anther, Physiological characteristics, Pollen traits

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