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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 40-47.doi: 10.3724/SP.J.1006.2011.00040

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Structure and Expression Analysis of Two Novel Genes Encoding β-glucanase in Cotton

DONG Jia,CAI Cai-Ping,WANG Li-Ke,ZHAO Liang,ZHANG Tian-Zhen,GUO Wang-Zhen*   

  1. National Key Laboratory of Crop Genetics & Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-06-10 Revised:2010-08-04 Online:2011-01-12 Published:2010-11-12
  • Contact: 郭旺珍, E-mail: moelab@njau.edu.cn

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Abstract: The β-glucanase is a type of enzyme degrading β-glucan. Cloning and expression analysis of genes encoding β-glucanase can provide information in both gene resources and breeding utilization for improvement of cotton fiber quality. The two novel genes encoding β-glucanase, designated as GhEG (GenBank accession No: HM462003) and GhGLU (GenBank accession No: HM462004), were obtained by sillico cloning and cDNA library screening, respectively. GhEG contained an open reading frame of 1 581 bp that encoded a polypeptide of 526 amino acids, and GhGLU contained an open reading frame of 1 410 bp that encoded a polypeptide of 469 amino acids. The genome sequence indicated that GhEG has five introns and six exons, while GhGLU has no intron and only one exon. The two genes all had one copy in diploid cotton species G. herbaceum and G. raimondii and two copies in tetraploid cotton species G. hirsutum acc. TM-1 and G. barbadense cv. Hai 7124, respectively. One of GhEG or GhGLU homoelogs in tetraploid was located on chromosome 19 and chromosome 4 by developing SNP marker, respectively. Q-PCR expression analysis showed that GhEG was expressed specifically in fiber elongation and had obvious difference between TM-1 and Hai7124 at the fiber elongation period of 15 DPA and 20 DPA, almost no transcripts were detected in root, stem and leaf. GhGLU was expressed in all tissues, dominantly in root, at fiber initiation and fiber late elongation phase, with obvious difference between TM-1 and Hai7124.

Key words: GhEG, GhGLU, Structure, Expression

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