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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 1116-1123.doi: 10.3724/SP.J.1006.2011.01116

• RESEARCH NOTES • Previous Articles    

Establishment of DNA Fingerprintings of Kenaf (Hibiscus Cannabinus L.) Germplasm Resources with ISSR Molecular Markers

WANG Bin1,**,QI Wei1,**,LAN Tao1,CHEN Hui-Duan2,XU Jian-Tang1,SU Jian-Guang3,LI Ai-Qing4,QI Jian-Ming1,*   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; 4 Anhui Province Seeds Management Station, Hefei 230001, China
  • Received:2010-09-16 Revised:2011-03-06 Online:2011-06-12 Published:2011-03-24
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-83644898

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Abstract: Kenaf (Hibiscus cannabinus L.) is an important economic crop in China. To identify germplasm resources and establish the data base of DNA fingerprintings, we adopted six kenaf germplasm resources to screen 80 ISSR primers, 20 of which were polymorphic. Then 20 ISSR primers were used to amplify 84 kenaf germplasm resources introduced and preserved from home and abroad. Totally 230 bands were produced,the average number of DNA bands amplified by each primer was 11.5, and the number of polymorphic DNA bands was 185. The polymorphic proportion of DNA bands was 80.43%, which indicated the abundant genetic diversity of kenaf germplasm resources preserved. Based on the DNA bands amplified from 84 kenaf germplasm resources, the Excel data base for PCR amplified bands of the kenaf germplasm resources was established. According to the principle of uniqueness of the fingerprintings, the DNA fingerprints of 82 kenaf germplasmwere constructed with five ISSR primers (UBC813, UBC825, UBC836, UBC888 and UBC889) selected from 20 polymorphic primers, using the analyzing software designed by our laboratory programmed and based on image manipulation. The fingerprintings provide a basis of the molecular identification of kenaf germplasm resources. In this research, the DNA fingerprintings system was proved to be feasible and reliable.

Key words: Kenaf (Hibiscus cannabinus L.), Germplasm resource, DNA, ISSR, Fingerprinting

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