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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (11): 2117-2121.doi: 10.3724/SP.J.1006.2011.02117

• RESEARCH NOTES • Previous Articles    

Detection of Genetically Modified Maize MON88017 by Quantitative Real-Time PCR

YUAN Lei1,2,SUN Hong-Wei1,LI Fan1,LI Ning1,3,ZHAO Lei3,*,LU Xing-Bo1,*   

  1. 1 Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China; 2 Department of Food Engineering, Shandong Business Institute, Yantai 264670, China; 3 College of Life Science, Shandong Normal University, Ji’nan 250014, China
  • Received:2011-04-01 Revised:2011-07-15 Online:2011-11-12 Published:2011-09-06
  • Contact: 路兴波, E-mail: luxb99@sina.com, Tel: 0531-83179095; 赵蕾, E-mail: zhaolei@sdu.edu.cn, Tel: 0531-88177190

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Abstract: Transgenic event-specific primers and Taqman probes based on the left-flanking sequence of MON88017 and endogenous gene (zSSIIb) were designed, and a real-time PCR method was developed to quantitatively detect the event-specific genetically modified maize. The reference molecule composed of endogenous gene and flanking sequence of MON88017 was constructed artificially. Two standard curves of the reference gene sequence and the 5' flanking sequence were established. We detected five mixed samples their genetically modified contents of MON88017 were 0.01%, 0.05%, 0.1%, 0.5%, and 1% respectively. The lowest detection limit was 19–30 copies. This study indicated that the Taqman probes real-time PCR is highly specific and sensitive.

Key words: Genetically modified organism, Real-time PCR, MON88017 event, Reference molecule

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