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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1847-1855.doi: 10.3724/SP.J.1006.2012.01847


Isolation and Molecular Characterization of Stress-Related TaLEAL3 Gene in Wheat

MIN Dong-Hong1,2,ZHAO Yue1,CHEN Yang1,XU Zhao-Shi2,*,HUO Dong-Ying1,HU Di1,CHEN Ming2,LI Lian-Cheng2,MA You-Zhi2   

  1. 1College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China
  • Received:2012-02-13 Revised:2012-04-20 Online:2012-10-12 Published:2012-07-27
  • Contact: 徐兆师, E-mail: xuzhaoshi@yahoo.com.cn, Tel: 010-82106773


Group 3 LEA proteins are proved to mediate plant responses to abiotic stresses such as drought, low temperature, and high salt. However, the LEA genes from common wheat (Triticum aestivum L.) have been rarely studied. We cloned a LEA gene, designated TaLEAL3, from the cDNA library of drought-treated wheat seedlings using phage hybridization in situ. The TaLEAL3 gene is 750 bp in full length and has a 501 bp open reading frame (ORF) encoding 166 amino acids. Based on multiple sequence alignment, TaLEAL3 was found to have the LEA structure characterized by α-helix and three incomplete repeat motifs comprising 11-mer amino acids. The result of electronic mapping showed that TaLEAL3 was located on chromosomes 4BL, 4DL, and 5AL. This gene was mainly expressed in stems but almost not in roots. Besides, the expression of TaLEAL3 was induced markedly by drought, low-temperature, and exogenous abscisic acid. Promoter analysis showed that the core promoter elements and cis-acting elements responding to drought and low-temperature stresses were found in the region of 1.7 kb upstream of TaLEAL3 gene. These results provided experimental data for further studying the function of LEA genes and the mechanism of LEA proteins.

Key words: Wheat, LEA protein, Real-time PCR, Subcellular localization, Promoter isolation

[1]Xiong L, Schumaker K S, Zhu J K. Cell signaling during cold, drought, and salt stress. Plant Cell, 2002, 14: 165–183

[2]Xu Z S, Chen M, Li L C, Ma Y Z. Functions of the ERF transcription factor family in plants. Botany, 2008, 86: 969–977

[3]Dure L, Chlan C. Developmental biochemistry of cottonseed embryogenesis and germination: XII. Purification and properties of principal storage proteins. Plant Physiol, 1981, 68: 180–186

[4]Ramanjulu S, Bartels D. Drought- and desiccation-induced modulation of gene expression in plant. Plant Cell Environ, 2002, 25: 141–151

[5]Hollung K, Espelund M, Jakobsen K S. Another Lea B19 gene (Group 1 Lea) from barley containing a single 20 amino acid hydrophilic motif. Plant Mol Biol, 1994, 25: 559–564

[6]Dure III L. A repeating 11-mer amino acid motif and plant dessication. Plant J, 1993, 3: 363–369

[7]Close T J. Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Plant Physiol, 1996, 97: 795–803

[8]Danyluk J, Perron A, Houde M, Limin A, Fowler B, Benhamou N, Sarhan F. Accumulation of an acidic dehydrin in the vicinity of the plasma membrance during cold acclimation of wheat. Plant Cell, 1988, 10: 623–638

[9]Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 377–403

[10]Close T J. Dehydrins: a commonalty in the response of plants to dehydration and low temperature. Physiol Plant, 1997, 100: 291–296

[11]Dure III L, Crouch M, Harada J, Ho T H D, Mundy J, Quatrano R, Thomas T, Sung Z R. Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol, 1989, 12: 475–486

[12]Dure L III. A repeating 11-mer amino acid motif and plant desiccation. Plant J, 1993, 3: 363–369

[13]Bray E A. Molecular responses to water deficit. Plant Physiol, 1993, 103: 1035–1040

[14]Sunderlíková V, Wilhelm E. High accumulation of legumin and Lea-like mRNAs during maturation is associated with increased conversion frequency of somatic embryos from pedunculate oak (Quercus robur L.). Protoplasma, 2002, 220: 97–103

[15]Tunnacliffe A, Wise M J. The continuing conundrum of the LEA proteins. Naturwissenschaften, 2007, 94: 791–812

[16]Wang B F, Wang Y C, Zhang D W, Li H Y, Yang C P. Verification of the resistance of a LEA gene from Tamarix expression in Saccharomyces cerevisiae to abiotic stresses. J For Res, 2008, 19: 58–62

[17]Vaseva II, Grigorova B S, Simova-Stoilova L P, Demirevska K N, Feller U. Abscisic acid and late embryogenesis abundant protein profile changes in winter wheat under progressive drought stress. Plant Biol (Stuttg), 2010, 12: 698–707

[18]Kramer D, Breitenstein B, Kleinwächter M, Selmar D. Stress metabolism in green coffee beans (Coffea arabica L.): expression of dehydrins and accumulation of GABA during drying. Plant Cell Physiol, 2010, 51: 546–553

[19]Baker J, Steele C, Dure L. Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Mol Biol, 1988, 11: 277–291

[20]Borovskii G B, Stupnikova I V, Antipina A I, Downs C A, Voinikov V K. Accumulation of dehydrin-like proteins in the mitochondria of cold-treated plants. J Plant Physiol, 2000, 156: 797–800

[21]Richard S, Morency M, Drevet C, Jouanin L, Séguin A. Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses. Plant Mol Biol, 2000, 43: 1–10

[22]Franco O L, Melo F R. Osmoprotectants: a plant strategy in response to osmotic stress. Russ J Plant Physiol, 2000, 47: 137–144

[23]Allagulova C R, Gimalov F R, Shakirova F M, Vakhitov V A. The plant dehydrins: structure and putative functions. Biochemistry, 2003, 68: 945–951

[24]Straub P F, Shen Q, Ho T D. Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1. Plant Mol Biol, 1994, 26: 617–630

[25]Xu D, Duan X, Wang B, Hong B, Ho T, Wu R. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol, 1996, 110: 249–257

[26]Lal S, Gulyani V, Khurana P. Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica). Transgenic Res, 2008, 17: 651–663

[27]Dalal M, Tayal D, Chinnusamy V, Bansal K C. Abiotic stress and ABA-inducible group 4 LEA from Brassica napus plays a key role in salt and drought tolerance. J Biotechnol, 2009, 139: 137–145

[28]Ried J L, Walker-Simmons M K. Group 3 late embryogenesis abundant proteins in desiccation-tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiol, 1993, 102: 125–131

[29]Hundertmark M, Hincha D K. LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics, 2008, 9: 118

[30]Tsuda K, Tsvetanov S, Takumi S, Mori N, Atanassov A, Nakamura C. New members of a cold-responsive group-3 Lea/Rab-related Cor gene family from common wheat (Triticum aestivum L.). Genes Genet Syst, 2000, 75: 179–188

[31]Xu Z S, Xia L Q, Chen Ming, Cheng X G, Zhang R Y, Li L C, Zhao Y X, Lu Y, Ni Z Y, Liu L, Qiu Z G, Ma Y Z. Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance. Plant Mol Biol, 2007, 65: 719–732

[32]Welin B V, Olson A, Nylander M, Palva E T. Characterization and differential expression of dhn/lea/rab-like genes during cold-acclimation and drought stress in Arabidopsis thaliana. Plant Mol Biol, 1994, 26: 131–144

[33]Bray E A. Plant responses to water deficit. Trends Plant Sci, 1997, 25: 48–54

[34]Moons A, De Keyser A, Van Montagu M. A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response. Gene, 1997, 191: 197–204

[35]Li L, Shimada T, Takahashi H, Ueda H, Fukao Y, Kondo M, Nishimura M, Hara-Nishimura I. MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell, 2006, 18: 3535–3547

[36]Abdo M, Hisheh S, Arfuso F, Dharmarajan A. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression. Reprod Biol Endocrinol, 2008, 6: 50

[37]Speulman E, Salamini F. GA3-regulated cDNAs from Hordeum vulgare leaves. Plant Mol Biol, 1995, 28: 915–926

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