Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1847-1855.doi: 10.3724/SP.J.1006.2012.01847

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Molecular Characterization of Stress-Related TaLEAL3 Gene in Wheat

MIN Dong-Hong1,2,ZHAO Yue1,CHEN Yang1,XU Zhao-Shi2,*,HUO Dong-Ying1,HU Di1,CHEN Ming2,LI Lian-Cheng2,MA You-Zhi2   

  1. 1College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China
  • Received:2012-02-13 Revised:2012-04-20 Online:2012-10-12 Published:2012-07-27
  • Contact: 徐兆师, E-mail: xuzhaoshi@yahoo.com.cn, Tel: 010-82106773

Abstract:

Group 3 LEA proteins are proved to mediate plant responses to abiotic stresses such as drought, low temperature, and high salt. However, the LEA genes from common wheat (Triticum aestivum L.) have been rarely studied. We cloned a LEA gene, designated TaLEAL3, from the cDNA library of drought-treated wheat seedlings using phage hybridization in situ. The TaLEAL3 gene is 750 bp in full length and has a 501 bp open reading frame (ORF) encoding 166 amino acids. Based on multiple sequence alignment, TaLEAL3 was found to have the LEA structure characterized by α-helix and three incomplete repeat motifs comprising 11-mer amino acids. The result of electronic mapping showed that TaLEAL3 was located on chromosomes 4BL, 4DL, and 5AL. This gene was mainly expressed in stems but almost not in roots. Besides, the expression of TaLEAL3 was induced markedly by drought, low-temperature, and exogenous abscisic acid. Promoter analysis showed that the core promoter elements and cis-acting elements responding to drought and low-temperature stresses were found in the region of 1.7 kb upstream of TaLEAL3 gene. These results provided experimental data for further studying the function of LEA genes and the mechanism of LEA proteins.

Key words: Wheat, LEA protein, Real-time PCR, Subcellular localization, Promoter isolation

[1]Xiong L, Schumaker K S, Zhu J K. Cell signaling during cold, drought, and salt stress. Plant Cell, 2002, 14: 165–183



[2]Xu Z S, Chen M, Li L C, Ma Y Z. Functions of the ERF transcription factor family in plants. Botany, 2008, 86: 969–977



[3]Dure L, Chlan C. Developmental biochemistry of cottonseed embryogenesis and germination: XII. Purification and properties of principal storage proteins. Plant Physiol, 1981, 68: 180–186



[4]Ramanjulu S, Bartels D. Drought- and desiccation-induced modulation of gene expression in plant. Plant Cell Environ, 2002, 25: 141–151



[5]Hollung K, Espelund M, Jakobsen K S. Another Lea B19 gene (Group 1 Lea) from barley containing a single 20 amino acid hydrophilic motif. Plant Mol Biol, 1994, 25: 559–564



[6]Dure III L. A repeating 11-mer amino acid motif and plant dessication. Plant J, 1993, 3: 363–369



[7]Close T J. Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Plant Physiol, 1996, 97: 795–803



[8]Danyluk J, Perron A, Houde M, Limin A, Fowler B, Benhamou N, Sarhan F. Accumulation of an acidic dehydrin in the vicinity of the plasma membrance during cold acclimation of wheat. Plant Cell, 1988, 10: 623–638



[9]Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 377–403



[10]Close T J. Dehydrins: a commonalty in the response of plants to dehydration and low temperature. Physiol Plant, 1997, 100: 291–296



[11]Dure III L, Crouch M, Harada J, Ho T H D, Mundy J, Quatrano R, Thomas T, Sung Z R. Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol, 1989, 12: 475–486



[12]Dure L III. A repeating 11-mer amino acid motif and plant desiccation. Plant J, 1993, 3: 363–369



[13]Bray E A. Molecular responses to water deficit. Plant Physiol, 1993, 103: 1035–1040



[14]Sunderlíková V, Wilhelm E. High accumulation of legumin and Lea-like mRNAs during maturation is associated with increased conversion frequency of somatic embryos from pedunculate oak (Quercus robur L.). Protoplasma, 2002, 220: 97–103



[15]Tunnacliffe A, Wise M J. The continuing conundrum of the LEA proteins. Naturwissenschaften, 2007, 94: 791–812



[16]Wang B F, Wang Y C, Zhang D W, Li H Y, Yang C P. Verification of the resistance of a LEA gene from Tamarix expression in Saccharomyces cerevisiae to abiotic stresses. J For Res, 2008, 19: 58–62



[17]Vaseva II, Grigorova B S, Simova-Stoilova L P, Demirevska K N, Feller U. Abscisic acid and late embryogenesis abundant protein profile changes in winter wheat under progressive drought stress. Plant Biol (Stuttg), 2010, 12: 698–707



[18]Kramer D, Breitenstein B, Kleinwächter M, Selmar D. Stress metabolism in green coffee beans (Coffea arabica L.): expression of dehydrins and accumulation of GABA during drying. Plant Cell Physiol, 2010, 51: 546–553



[19]Baker J, Steele C, Dure L. Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Mol Biol, 1988, 11: 277–291



[20]Borovskii G B, Stupnikova I V, Antipina A I, Downs C A, Voinikov V K. Accumulation of dehydrin-like proteins in the mitochondria of cold-treated plants. J Plant Physiol, 2000, 156: 797–800



[21]Richard S, Morency M, Drevet C, Jouanin L, Séguin A. Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses. Plant Mol Biol, 2000, 43: 1–10



[22]Franco O L, Melo F R. Osmoprotectants: a plant strategy in response to osmotic stress. Russ J Plant Physiol, 2000, 47: 137–144



[23]Allagulova C R, Gimalov F R, Shakirova F M, Vakhitov V A. The plant dehydrins: structure and putative functions. Biochemistry, 2003, 68: 945–951



[24]Straub P F, Shen Q, Ho T D. Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1. Plant Mol Biol, 1994, 26: 617–630



[25]Xu D, Duan X, Wang B, Hong B, Ho T, Wu R. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol, 1996, 110: 249–257



[26]Lal S, Gulyani V, Khurana P. Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica). Transgenic Res, 2008, 17: 651–663



[27]Dalal M, Tayal D, Chinnusamy V, Bansal K C. Abiotic stress and ABA-inducible group 4 LEA from Brassica napus plays a key role in salt and drought tolerance. J Biotechnol, 2009, 139: 137–145



[28]Ried J L, Walker-Simmons M K. Group 3 late embryogenesis abundant proteins in desiccation-tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiol, 1993, 102: 125–131



[29]Hundertmark M, Hincha D K. LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics, 2008, 9: 118



[30]Tsuda K, Tsvetanov S, Takumi S, Mori N, Atanassov A, Nakamura C. New members of a cold-responsive group-3 Lea/Rab-related Cor gene family from common wheat (Triticum aestivum L.). Genes Genet Syst, 2000, 75: 179–188



[31]Xu Z S, Xia L Q, Chen Ming, Cheng X G, Zhang R Y, Li L C, Zhao Y X, Lu Y, Ni Z Y, Liu L, Qiu Z G, Ma Y Z. Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance. Plant Mol Biol, 2007, 65: 719–732



[32]Welin B V, Olson A, Nylander M, Palva E T. Characterization and differential expression of dhn/lea/rab-like genes during cold-acclimation and drought stress in Arabidopsis thaliana. Plant Mol Biol, 1994, 26: 131–144



[33]Bray E A. Plant responses to water deficit. Trends Plant Sci, 1997, 25: 48–54



[34]Moons A, De Keyser A, Van Montagu M. A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response. Gene, 1997, 191: 197–204



[35]Li L, Shimada T, Takahashi H, Ueda H, Fukao Y, Kondo M, Nishimura M, Hara-Nishimura I. MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell, 2006, 18: 3535–3547



[36]Abdo M, Hisheh S, Arfuso F, Dharmarajan A. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression. Reprod Biol Endocrinol, 2008, 6: 50



[37]Speulman E, Salamini F. GA3-regulated cDNAs from Hordeum vulgare leaves. Plant Mol Biol, 1995, 28: 915–926

[1] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[3] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[4] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[5] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[6] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[7] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!