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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (09): 1701-1709.doi: 10.3724/SP.J.1006.2013.01701

• RESEARCH NOTES • Previous Articles    

Molecular Cloning and Bioinformatics Analysis of K+ Transporter Gene (GmKT12) from Soybean (Glycine max [L.] Merri)

ZHU Xiao-Ling1,2,CHEN Hai-Feng1,WANG Cheng1,3,HAO Qing-Nan1,2,CHEN Li-Miao1,GUO Dan-Dan1,2,WU Bao-Duo1,CHEN Shui-Lian1,SHA Ai-Hua1,ZHOU Rong1,*,ZHOU Xin-An1,*   

  1. 1 Oil Crops Research Institute of Chinese Academy of Agriculture Sciences / Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan 430062, China; 2Graduate School of Chinese Academy of Agriculture Sciences, Beijing 100081, China; 3 Nanjing Agricultural University / National Center for Soybean Improvement/ National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
  • Received:2012-10-15 Revised:2013-04-22 Online:2013-09-12 Published:2013-07-09
  • Contact: 周蓉, E-mail: zhourong@oilcrops.cn, Tel: 027-86735887; 周新安, E-mail: zhouxa@oilcrops.cn, Tel: 027-86711563

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Abstract:

GmKT12 predicted as a potassium transporter is of great importance in absorbing nutrients, signal transduction in plant growth. At present, there are few reports about the gene. In this paper, one low K tolerant soybean line You 06-71 and one low K intolerant line Hengchun 04-11 were weed as plant materials. In order to research the expression level of GmKT12 in low potassium stress conditions, RNA was extracted and tested by Real-time PCR in eight periods. The results showed that there were highly significant differences between two lines due to their was diversity of amino acid sequence and protein structure. Homology and Bioinformatic analysis after cloning the target sequence from the two lines showed that comparing with GmKT12,there were 56 homologous genes with more than 30 percent similarity, GmKT12 andGlyma18g18822 were nearest in the phylogenetic tree. The protein encoded by GmKT12 was soluble transmembrane protein which contained 11–12 membrane structural domains, and possessed multiple phosphorylation sites. These indicated that the protein might take part in plant signal transduction and regulate potassium ions transportation into or out of cells. In conclusion, GmKT12 might play a pivotal role in potassium absorption in soybean, on which the research is important.

Key words: Soybean [Glycine max L. Merri], Potassium transporter, Cloning, Bioinformatics analysis, Real-time PCR, Sequencing, Homologous analysis, Phylogenetic analysi

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