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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1253-1258.doi: 10.3724/SP.J.1006.2016.01253

• RESEARCH NOTES • Previous Articles    

Cloning and Functional Analysis of wzy2-1Gene in Wheat

QIANG Zhi-Quan1,LIANGYa-Jun1,YU Zheng-Yang1,DUYa1,ZHANGShuai1,ZHUWei-Ning2,ZHANG Lin-Sheng1,*   

  1. 1College of Life Science/ State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University,Yangling712100,China; 2College of Life Science, Northwest University, Xi’an 710069, China
  • Received:2016-01-23 Revised:2016-05-09 Online:2016-08-12 Published:2016-06-02
  • Contact: 张林生, E-mail: linszhang@nwsuaf.edu.cn, Tel: 029-87092379 E-mail:qiangsir0934@qq.com
  • Supported by:

    This study was supported by the Projects for Doctoral Research Funding in Higher Education InstitutionsFunction and Structure of Dehydrin Protein in Different Water Content (20120204110033) and the Foundation of State Key Laboratory of Crop Stress Biology for Arid Areas of Northwest A&F University (CSBAA2015007).

Abstract:

Dehydrins (DHNs) are identified as the group II of LEA proteins and involved in plant abiotic stress tolerance.In this study,we isolated a novel Kn-type dehydrin genefrom wheat cultivarZhengyin 1, which was designated wzy2-1.The full length of wzy2-1 is 1740bp,encoding 579 amino acids andcontaining nine conserved K-fragments.Sequence alignment indicated that wzy2-1hadhighhomologytoDhn5gene in Hordeumvulgare.The WZY2-1 protein waspredicted to be a highly-hydrophilic and disordered protein. The WZY2-1 protein was successfully expressed in E. coli strain BL21 (DE3). We found that WZY2-1 protein improved the tolerance to low or high temperature, salt and osmotic stressesin E. coli. The qRT-PCR assay indicated that the expression of wzy2-1gene was inducedby low temperature, PEG, and salt stresses rather than ABA. Thus, we conclude that wzy2-1 is an ABA-independent gene.

Key words: Wheat, Dehydrins, Real-time PCR, Procaryoticexpression

[1] 闵东红, 赵月, 陈阳, 徐兆师, 霍冬英, 胡笛, 陈明, 李连城, 马有志. 小麦胁迫相关基因TaLEA3的克隆及分子特性分析. 作物学报, 2012,10:1847–1855
Min D H, Zhao Y, Chen Y, Xu Z S, Huo D Y, Hu D, Chen M, Li L C, Ma Y Z. Isolation and molecular characterization of stress-related TaLEA3 gene in wheat. ActaAgron Sin, 2012, 10: 1847?1855 (in Chinese with English abstract)
[2] Furuki T, Sakurai M. Group 3 LEA protein model peptides protect liposomes during desiccation. BiochimBiophysActa, 2014, 11: 2757?2766
[3] 王康, 朱慧森, 董宽虎. 植物LEA蛋白及其基因家族成员PM16研究进展. 草业科学, 2008, (2): 97?102
Wang K, Zhu H S, Dong K H. Research progress on molecular biology of LEA protein and members of LEA gene family-PM16. PratacultSci, 2008, (2): 97?102(in Chinese with English abstract)
[4] Lii D L. Structural motifs in lea proteins. Curr Topics Plant Physiol, 1993, 10: 91?103
[5] Liu H, Yu C, Li H, Ouyang B, Wang T, Zhang J, Wang X, Ye Z. Overexpression of ShDHN, a dehydrin gene from Solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato. Plant Sci, 2015, 231: 198?211
[6] Close T J. Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant, 1996, 97: 795?803
[7] Zolotarov Y, Stromvik M. De novo regulatory motif discovery identifies significant motifs in promoters of five classes of plant dehydrin genes. PloS One, 2015, 10: e0129016
[8] Godoy J A, Lunar R, Torres-Schumann S, Moreno J, Rodrigo R M, Pintor-Toro J A. Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants. Plant MolBiol, 1994, 26: 1921?1934
[9] Jensen A B, Goday A, Figueras M, Jessop A C, Pages M. Phosphorylation mediates the nuclear targeting of the maize Rab17 protein. Plant J, 1998, 13: 691?697
[10] Carpenter J F, Crowe J H. The mechanism of cryoprotection of proteins by solutes. Cryobiology, 1988, 25: 244?255
[11] Drira M, Saibi W, Amara I, Masmoudi K, Hanin M, Brini F. Wheat dehydrin K-segments ensure bacterial stress tolerance, antiaggregation and antimicrobial effects. ApplBiochemBiotechnol, 2015, 175: 3310?3321
[12] M, Saibi W, Brini F, Gargouri A, Masmoudi K, Hanin M. The K-segments of the wheat dehydrin DHN-5 are essential for the protection of lactate dehydrogenase and beta-glucosidase activities in vitro. MolBiotechnol, 2013, 54: 643?650
[13] Yang W, Zhang L, Lv H, Li H, Zhang Y, Xu Y, Yu J. The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress. Front Plant Sci, 2015, 6: 406
[14] Tsvetanov S, Ohno R, Tsuda K, Takumi S, Mori N, Atanassov A, Nakamura C. A cold-responsive wheat (TriticumaestivumL.) gene wcor14 identified in a winter-hardy cultivar “Mironovska 808”. Genes Genet Syst, 2000, 75: 49?57
[15] Baker S S, Wilhelm K S, Thomashow M F. The 5′-region of Arabidopsis thalianacor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant MolBiol,1994, 24: 701?713
[16] Close T J. Dehydrins: a commonalty in the response of plants to dehydration and low temperature. Physiol Plant, 1997, 100: 291–296
[17] Kosova K, Vitamvas P, Prasil I T. Wheat and barley dehydrins under cold, drought, and salinity: what can LEA-II proteins tell us about plant stress response? Front Plant Sci, 2014, 5: 343
[18] Chen R G, Jing H, Guo W L, Wang S B, Ma F, Pan B G, Gong Z H. Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L. Plant Cell Rep, 2015, 34: 2189?2200
[19]刘亚玲, 王俊杰, 云锦凤, 赵彦, 侯永霞. 黄花苜蓿LEA3基因片段克隆与生物信息学分析. 生物技术通报, 2011, (7): 82?87
Liu Y L , Wang J J, Yun J F, Zhao Y, Hou Y X. Cloning and bioinformatics analysis of Lea3gene from wild Medicago falcate. Biotechnol Bull, 2011, (7): 82?87(in Chinese with English abstract)
[20] 杜俊波, 席德慧, 王尚英, 冯鸿, 孙歆, 袁澍, 王建辉, 刘自礼, 薛立微, 林宏辉. 青稞脱水素基因dhn4的克隆与原核表达. 四川大学学报(自然科学版), 2008,45: 441?445
Du J B, Kang D H, Wang S Y, Feng H, Sun Y, Yuan S, Wang J H, Liu Z L, Xue L W, Lin H H. Cloning and procaryotic expression of the dehydrin dhn4 gene from Tibetan hulless barley. J Sichuan Univ (Nat SciEdn), 2008, 45: 441?445 (in Chinese with English abstract)
[21] Kalemba E M, Litkowiec M. Functional characterization of a dehydrin protein from Fagus sylvatica seeds using experimental and in silico approaches. Plant Physiol&Biochem, 2015, 97: 246?254
[22] Wang M, Li P, Li C, Pan Y, Jiang X, Zhu D, Zhu D, Zhao Q and Yu J. SiLEA14, a novel atypical LEA protein, confers abiotic stress resistance in foxtail millet. BMC Plant Biol, 2014, 14: 290?306
[23] Choi D W, Zhu B, Close T J. The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. TheorAppl Genet, 1999, 98: 1234?1247
[24] Garay-Arroyo A, Colmenoro-Florest J M, Garciarrubio A, Covarrubias A A. Highly hydrophilic proteins in prokaryotes and eucaryotes are common during conditions of water deficit. BiolChem, 2000, 275: 5668?5674
[25] Nylander M, Svensson J, Palva E T, Welin B V. Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant MolBiol, 2001, 45: 263?279
[26] Davidson W S, Jonas A, Clayton D F, George, J M. Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes. BiolChem, 1998, 273: 9443?9449
[27] Popova A V, Rausch S, Hundertmark M, Gibon Y, Hincha D K. The intrinsically disordered protein LEA7 from Arabidopsis thaliana protects the isolated enzyme lactate dehydrogenase and enzymes in a soluble leaf proteome during freezing and drying. BiochimBiophysActa, 2015, 1854: 1517?1525
[28] Shinozaki K, Yamaguchi-Shinozaki K. Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. CurrOpin Plant Biol, 2000, 3: 217?223
[29] Giordani T, Natali L, D’Ercole A, Pugliesi C, Fambrini M, Vernieri P, Vitagliano C, Cavallini A. Expression of a dehydrin gene during embryo development and drought stress in ABA-deficient mutants of sunflower (Helianthus annuusL.). Plant MolBiol, 1999, 39: 739?748
[30] Zhu W N, Zhang D P, Lu X X,Zhang L S, Yu Z Y,Lv H, Zhang H M. Characterisation of an SKn-type dehydrin promoter from wheat and its responsiveness to various abiotic and biotic stresses. Plant MolBiol Rep, 2014, 32:664–678

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