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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1247-1252.doi: 10.3724/SP.J.1006.2016.01247

• RESEARCH NOTES • Previous Articles     Next Articles

Rapid Development of Glu-1 Locus Near-isogenic Introgression Lines Using HMW-GS Deletion Mutant

ZHANG Xing-Xing1,2,**,WANG Zhao-Jun2,3,**,YANG Yu-Shuang2,4,WANG Dao-Wen2,ZHENG Wen-Ming1,*,DONG Zhen-Ying2,*   

  1. 1State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2State Key Laboratory of PlantCell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3University of Chinese Academy of Sciences, Beijing 100049,China; 4Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571731, China
  • Received:2016-02-22 Revised:2016-05-09 Online:2016-08-12 Published:2016-06-02
  • Contact: 郑文明, E-mail: wmzheng@henau.edu.cn; 董振营, E-mail: zhydong@genetics.ac.cn E-mail:xingzhang.1989@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31300280) and the National Key Basic Research Program of China (2013CB127702).

Abstract:

Wheat (Triticumaestivum L., AABBDD) highmolecular weight glutenin subunits (HMW-GS) were encoded by the genes located inGlu-A1, Glu-B1and Glu-D1 loci.Evaluation and optimization of the combination of HMW-GS are very importantto understand Glu-1functions. In this study, we constructed aHMW-GS deletion mutant, DLGlu1 withXiaoyan 81 background, and crossed it with Glenlea, a Canada elite wheat variety with superior end-use quality. Combining the technologies of wheat embryo culture and molecular marker-assisted selection (MAS), we obtained seven introgression lines containing GlenleaGlu-A1a, Glu-B1al, and Glu-D1d loci, which can be developed as a complete set of near-isogenic introgression lines possessingGlenleadifferentHMW-GS genes. Our study indicated that the Glu-1 deletion mutant DLGlu1is of great value in the fast development of Glu-1 near-isogenic introgression lines and the studyand utility of wheat Glu-1.

Key words: Wheat, HMW-GS, Deletion mutant, Introgression lines

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