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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1253-1258.doi: 10.3724/SP.J.1006.2016.01253

• RESEARCH NOTES • Previous Articles    

Cloning and Functional Analysis of wzy2-1Gene in Wheat

QIANG Zhi-Quan1,LIANGYa-Jun1,YU Zheng-Yang1,DUYa1,ZHANGShuai1,ZHUWei-Ning2,ZHANG Lin-Sheng1,*   

  1. 1College of Life Science/ State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University,Yangling712100,China; 2College of Life Science, Northwest University, Xi’an 710069, China
  • Received:2016-01-23 Revised:2016-05-09 Online:2016-08-12 Published:2016-06-02
  • Contact: 张林生, E-mail: linszhang@nwsuaf.edu.cn, Tel: 029-87092379 E-mail:qiangsir0934@qq.com
  • Supported by:

    This study was supported by the Projects for Doctoral Research Funding in Higher Education InstitutionsFunction and Structure of Dehydrin Protein in Different Water Content (20120204110033) and the Foundation of State Key Laboratory of Crop Stress Biology for Arid Areas of Northwest A&F University (CSBAA2015007).

Abstract:

Dehydrins (DHNs) are identified as the group II of LEA proteins and involved in plant abiotic stress tolerance.In this study,we isolated a novel Kn-type dehydrin genefrom wheat cultivarZhengyin 1, which was designated wzy2-1.The full length of wzy2-1 is 1740bp,encoding 579 amino acids andcontaining nine conserved K-fragments.Sequence alignment indicated that wzy2-1hadhighhomologytoDhn5gene in Hordeumvulgare.The WZY2-1 protein waspredicted to be a highly-hydrophilic and disordered protein. The WZY2-1 protein was successfully expressed in E. coli strain BL21 (DE3). We found that WZY2-1 protein improved the tolerance to low or high temperature, salt and osmotic stressesin E. coli. The qRT-PCR assay indicated that the expression of wzy2-1gene was inducedby low temperature, PEG, and salt stresses rather than ABA. Thus, we conclude that wzy2-1 is an ABA-independent gene.

Key words: Wheat, Dehydrins, Real-time PCR, Procaryoticexpression

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