Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1247-1252.doi: 10.3724/SP.J.1006.2016.01247

• RESEARCH NOTES • Previous Articles     Next Articles

Rapid Development of Glu-1 Locus Near-isogenic Introgression Lines Using HMW-GS Deletion Mutant

ZHANG Xing-Xing1,2,**,WANG Zhao-Jun2,3,**,YANG Yu-Shuang2,4,WANG Dao-Wen2,ZHENG Wen-Ming1,*,DONG Zhen-Ying2,*   

  1. 1State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2State Key Laboratory of PlantCell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3University of Chinese Academy of Sciences, Beijing 100049,China; 4Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571731, China
  • Received:2016-02-22 Revised:2016-05-09 Online:2016-08-12 Published:2016-06-02
  • Contact: 郑文明, E-mail: wmzheng@henau.edu.cn; 董振营, E-mail: zhydong@genetics.ac.cn E-mail:xingzhang.1989@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31300280) and the National Key Basic Research Program of China (2013CB127702).

Abstract:

Wheat (Triticumaestivum L., AABBDD) highmolecular weight glutenin subunits (HMW-GS) were encoded by the genes located inGlu-A1, Glu-B1and Glu-D1 loci.Evaluation and optimization of the combination of HMW-GS are very importantto understand Glu-1functions. In this study, we constructed aHMW-GS deletion mutant, DLGlu1 withXiaoyan 81 background, and crossed it with Glenlea, a Canada elite wheat variety with superior end-use quality. Combining the technologies of wheat embryo culture and molecular marker-assisted selection (MAS), we obtained seven introgression lines containing GlenleaGlu-A1a, Glu-B1al, and Glu-D1d loci, which can be developed as a complete set of near-isogenic introgression lines possessingGlenleadifferentHMW-GS genes. Our study indicated that the Glu-1 deletion mutant DLGlu1is of great value in the fast development of Glu-1 near-isogenic introgression lines and the studyand utility of wheat Glu-1.

Key words: Wheat, HMW-GS, Deletion mutant, Introgression lines

[1] Arabidopsis Genome Initiative. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature,2000, 408: 796–815
[2] International Rice Genome Sequencing Project. The map-based sequence of the rice genome. Nature, 2005,436:793–800
[3] Brenchley R, Spannagl M, Pfeifer M, Barker GA, D’Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Hou NX, Luo MC, Sehgal S, Gill B, Kianian S, Anderson O, Kersey P, Dovrak J, McCombie WR, Hall A, Mayer KFX, Edwards K, Bevan MW, Hall N. Analysis of the bread wheat genome using whole-genome shotgun sequencing.Nature,2012, 491:705–710
[4] International Wheat Genome Sequencing Consortium. A chromosome-based draft sequence of the hexaploid bread wheat (Triticumaestivum) genome. Science,2014, 345: 1251788
[5] Jia J, Zhao S, Kong X, Li Y, Zhao G, He W, Appels R, Pfeifer M, Tao Y, Zhang X, Jing R, Zhang C, Ma Y, Gao L, Gao C, Spannagl M, Mayer KFX, Li D, Pan S, Zheng F, Hu Q, Xia X, Li J, Liang Q, Chen J, Wicker T, Gou C, Kuang H, He G, Luo Y, Keller B, Xia Q, Lu P, Wang J, Zou H, Zhang R, Xu J, Gao J, Middleton C, Quan Z, Liu G, Wang J, IWGSC, Yang H, Liu X, He Z, Mao L, Wang J.Aegilopstauschiidraft genome sequence reveals a gene repertoire for wheat adaptation.Nature, 2013, 496:91–95
[6]Ling HQ, Zhao S, Liu D, Wang J, Sun H, Zhang C, Fan H, Li D, L Dong, Tao Y, Gao C, Wu H, Li Y, Cui Y, Guo X, Zheng S, Wang B, Yu K, Liang Q, Yang W, Lou X, Chen J, Feng M, Jian J, Zhang X, Luo G, Jiang Y, Liu J, Wang Z, Sha Y, Zhang B, Wu H, Tang D, Shen Q, Xue P, Zou S, Wang X, Liu X, Wang F, Yang Y, An X, Dong Z, Zhang K, Zhang X, Luo MC, Dvorak J, Tong Y, Wang J, Yang H, Li Z, Wang D, Zhang A, Wang J.Draft genome of the wheat A-genome progenitor Triticumurartu. Nature, 2013, 496:87–90
[7] Li D, Dreher K, Knee E,Brkljacic J, Grotewold E, Berardini T Z, Lamesch P, Garcia-Hernandez M, Reiser L, Huala E.Arabidopsis Database and Stock Resources. Arabidopsis Protocols. Springer,2014.pp 65–96
[8] Hirochika H.Insertional mutagenesis with Tos17 for functional analysis of rice genes. Breed Sci, 2010, 60:486–492
[9] Wang TL, Uauy C, Robson F, Till B.TILLING in extremis. Plant Biotechnol J, 2012, 10: 761–772
[10] Fitzgerald TL, Powell JJ, Stiller J, Weese TL, Abe T, Zhao G,Jia J, McIntyreC L, Li Z, Manners J M, Kazan K. An assessment of heavy ion irradiation mutagenesis for reverse genetics in wheat (Triticumaestivum L.). PLoS One,2015, 10: e0117369
[11] Yang Y, Li S, Zhang K, Dong Z, Li Y, An X, Chen J, Chen Q, Jiao Z, Liu X, Qin H, Wang D. Efficient isolation of ion beam-induced mutants for homoeologous loci in common wheat and comparison of the contributions of Glu-1 loci to gluten functionality. TheorAppl Genet, 2014, 127:359–372
[12] Shewry PR, Halford NG.Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot, 2002, 53:947–958
[13]Shewry P R, Halford N G, Tatham A S, Popineau Y, Lafiandra D, Belton P S. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res, 2003, 45:219–302
[14] Lafiandra D, D’Ovidio R, Porceddu E, Margiotta B, Colaprico G.New data supporting high Mrglutenin subunit 5 as the determinant of quality differences among the pairs 5+10 vs 2+12. J Cereal Sci, 1993, 18:197–205
[15] Marchylo B A, Lukow O M, Kruger J E. Quantitative variation in high-molecular-weight glutenin subunit 7 in some Canadian wheats. J Cereal Sci, 1992, 5:29–37
[16] Sears E R. Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis K R eds. Chromosome Manipulation and Plant Genetics. Oliver & Boyd, Edinburgh, 1966. pp 29–45
[17] 孙果忠, 马民强, 柴建芳, 赵和, 谢晓亮, 王海波. 小麦幼胚培养与幼粒破眠的比较. 河北农业科学, 2000, 4(1): 58–61
Sun G Z, Ma M Q, Cai J F, Zhao H, Xie X L, Wang H B. Comparison on wheat embryo culture and dormancy-breaking. J Hebei AgrSci, 2000, 4(1): 58–61 (in Chinese with English abstract)
[18] Butow B J, Gale K R, Ikea J, Juhász A, Bedö Z, Tamás L, Gianibelli M C. Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. TheorAppl Genet, 2004, 109: 1525–1535
[19] Dong Z, Yang Y, Li Y, Zhang K , Lou H, An X, Dong L, Gu Y Q, Anderson O D, Liu X, Qin H, Wang D. Haplotype variation of Glu-D1 locus and the origin of Glu-D1d allele conferring superior end-use qualities in common wheat. PLoS One, 2013, 8: e74859
[20] Saghai-Maroof M A, Soliman KM, Jorgensen RA, Allard R W.Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl AcadSci USA,1984, 81:8014–8018
[21] Wan Y, Liu KF, Wang D, ShewryP R.High molecular weight subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat.TheorAppl Genet, 2000, 101: 879–884
[22] Zhang Y, Tang J W, Yan J, Zhang Y L, Zhang Y, Xia X C, He Z H.The gluten protein and interactions between components determine mixograph properties in an F6 recombinant inbred line population in bread wheat. J Cereal Sci,2009, 50:219–226
[23] Jin H, He Z H, Li G Y, Mu P Y, Fan Z R, Xia X C, Zhang Y.Effects of high molecular weight glutenin subunits on wheat quality by Aroona and its near-isogenic lines. SciAgric Sin,2013, 46:1095–1103
[24] Vasil IK, Bean S, Zhao JM, McCluskey P, Lookhart G, Zhao H P, Altpeter F, Vasil V.Evaluation of baking properties and gluten protein composition of field grown transgenic wheat lines expressing high molecular weight glutenin gene 1Ax1.J Plant Physiol. 2001, 158: 521–528
[25] Ma M, Yan Y, Huang L, Chen M, Zhao H. Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes. BMC Plant Biol,2012, 12:141
[26] PaynePI, Nightingale MA, KrattigerAF, Holt L M.The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric, 1987, 40: 51–65
[27] Branlard G, Dardevet M, Saccomano R,Lagoutte F, Gourdon J.Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica, 2001, 119:59–67
[28] Li Y, An X, Yang R, Guo X, Yue G, Fan R, Li B, Li Z, Zhang K, Dong Z, Zhang L, Wang J, Jia X, Ling H-Q, Zhang A, Zhang X, Wang D. Dissecting and enhancing the contributions of high-molecular-weight glutenin subunits to dough functionality and bread quality. Mol Plant, 2015, 8: 332–334
[29] 李保云, 刘桂芳, 王岳光, 孙辉, 刘广田. 小麦高分子量谷蛋白亚基的遗传规律研究.中国农业大学学报, 2000, 5(1): 58–62
Li B Y, Liu G F, Wang Y G, Sun H, Liu G T. Inheritance of high molecular weight glutenin subunits (HMW-GS) in wheat. J China AgricUniv, 2000, 5(1): 58–62 (in Chinese with English abstract)

[1] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[3] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[4] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[5] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[6] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[7] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!