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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2145-2151.doi: 10.3724/SP.J.1006.2011.02145


Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 21

MA Dong-Fang1,WANG Hai-Ge1,TANG Ming-Shuang1,YUAN Xi-Li1,BAI Yao-Bo1,ZHOU Xin-Li1,SONG Jian-Rong2,JING Jin-Xue1,*   

  1. 1 College of Plant Protection / State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, China; 2 Tianshui Municipal Institute of Agricultural Science of Gansu Province, Tianshui 741001, China
  • Received:2011-07-06 Revised:2011-09-16 Online:2011-12-12 Published:2011-09-29

Abstract: Wheat cultivar Zhongliang 21 displayed resistance to epidemic yellow rust races in China when tested with seven races of Puccinia striiformis f. sp. tritici (Pst). To use the elite resistance to Pst in wheat breeding, we identified the resistance gene (s) in Zhongliang 21 by means of phenotypic evaluation and molecular marker technique. Seedlings of the F1, F2, and BC1 generations from the cross between Zhongliang 21 (resistant) and Mingxian 169 (susceptible), as well as the parents, were tested with Pst race CYR30. The results showed that the stripe rust resistance in Zhongliang 21 was conferred by a single dominant gene, which was designated Yrzhong21, temporarily. Ten simple sequence repeat (SSR) markers located on chromosome arm 5AL, Xgwm186, Xbarc165, Xwmc795, Xbarc40, Xgwm156, Xgwm617, Xwmc415, Xbarc151, Xwmc338, and Xgwm666, were linked to Yrzhong21 with the nearest flanking markers of Xgwm186 and Xbarc165. The genetic distances were 7.5 cM to Xgwm186 and 2.7 cM to Xbarc165. Based on chromosomal location, reactions to various pathotypes andpedigree analysis, we deduced Yrzhong21 is a novel resistance gene to stripe rust. Eighteen cultivars (lines) of Zhongliang series were tested with the flanking markers Xgwm186 and Xbarc165, and only 17% showed the same banding pattern as that in Zhongliang 21. This result suggested that 17% of Zhongliang cultivars (lines) might carry Yrzhong21, indicating that this resistance gene has a potential application in wheat breeding program for strip rust resistance.

Key words: Zhongliang 21, Wheat stripe rust, Resistance gene, Genetic analysis, Molecular mapping

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