Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2152-2157.doi: 10.3724/SP.J.1006.2011.02152

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression of a Stress-induced GmPRP Gene in Soybean (Glycine max)

ZHAI Ying, LEI Ting-Ting,YAN Fan, HUANG Kai-Meng, LI Xiao-Wei, ZHANG Qing-Lin, ZHANG Hai-Jun, SU Lian-Tai, SUN Xin, WANG Ying, LI Jing-Wen*,WANG Qing-Yu*   

  1. College of Plant Sciences, Jilin University, Changchun 130062, China
  • Received:2011-05-10 Revised:2011-07-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 王庆钰, E-mail: wqy414cn@yahoo.com.cn; 李景文, E-mail: ljwk9@163.com

PDF

1987

Abstract: Plant proline-rich proteins (PRPs) are putative cell wall proteins, which are usually associated with different abiotic and biotic stress conditions. A soybean mRNA sequence encoding a proline-rich protein (PRP) was cloned and designated as GmPRP from Jilin 32 immature embryo gene expression profiles using RT-PCR. The GmPRP consisted of an ORF with a length of 396 bp, and encoded 131 amino acids (13.79 kD) with an isoelectric point of 8.96. There was no intron in the DNA sequence of GmPRP. Except a repetitive proline-rich domain, GmPRP also contained a signal peptide in the N-terminal domain and a conserved eight cysteine motif in the C-terminal domain. The amino acid sequences of GmPRP, PvPRP and CcHyPRP shared high homology through phylogenetic analysis. The length of the promoter was 683 bp, containing several stress-induced elements: ABRE-like,G-box,W-box,GT-1,MYB,MYC,BIHD10s,DPBF,SEBF, and WRKY. Real-time quantitative PCR (qPCR) analysis revealed that GmPRP expressed highly in root and leaf and low in flower. qPCR was also performed to investigate the expression profiles of the GmPRP under different stresses such as drought, high salt, low temperature, wound, SA (salicylic acid), ETH (ethane), ABA (abscisic acid) and MeJA (methyl jasmonate). Under these stresses GmPRP showed up-regulated expression patterns. These results revealed that GmPRP might be involved in multiple pathways of plants responding to the different environmental conditions.

Key words: GmPRP, Soybean, Adversity stresses, Promoter, Expression analysis

[1]Sakamato K, Tada Y, Yokozeki Y, Akagi H, Hayashi N, Fujimura T, Ichikawa N .Chemical induction of disease resistance in rice is correlated with the expression of a gene encoding a nucleotide binding site and leucine-rich repeats. Plant Mol Biol, 1999, 40: 847–855
[2]Komjanc M, Festi S, Rizzotti L, Cattivelli L, Cervone F, De Lorenzo G. A leucine-rich repeat receptor-like protein kinase (LRPKm1) gene is induced in Malus×domestica by Venturia inaequalis infection and salicylic acid treatment. Plant Mol Biol, 1999, 40: 945–57
[3]Li ZY, Chen SY. Differential accumulation of the S-adenosylmethionine decarboxylase transcript in rice seedlings in response to salt and drought stresses. Theor Appl Genet, 2000, 100: 782–788
[4]Merkouropoulos G, Barnett D C, Shirsat A H. The Arabidopsis extensin gene is developmentally regulated, is induced by wounding, methyl jamsmonate abscisic and salicylic acid, and codes for a protein with unusual motifs. Planta, 1999, 208: 212–219
[5]Showalter A M, Varner J E. Plant hydroxyproline-rich glycoproteins. Bioch Plant, 1989, 15: 485–520
[6]Jose-Estanyol M, Gomis-Rüth F X, Puigdomenech P. The eight-cysteine motif, a versatile structure in plant proteins. Plant Physiol Bioch, 2004, 42: 355–365
[7]Hong J C, Nagao R T, Key J L. Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell, 1989, 1: 937–43
[8]Deutch C E, Winicov I. Post-transcriptional regulation of a salt inducible alfalfa gene encoding a putative chimeric proline-rich cell wall protein. Plant Mol Biol, 1995, 27: 411–418
[9]Castonguay Y, Laberge S, Nadeau P, Vezina L P. A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins. Plant Mol Biol, 1994, 24: 799–804
[10]He C Y, Zhang J S, Chen S Y. A soybean gene encoding a proline rich protein is regulated by salicylic acid, an endogenous circadian rhythm and by various stresses. Theor Appl Genet, 2002, 104: 1125–1131
[11]Zhang Y, Schlappi M. Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast cells and form higher order complexes in plants. Planta, 2007, 227: 233–243
[12]Priyanka B, Sekhar K, Reddy V D, Rao K V. Expression of pigeonpea hybrid-proline-rich protein encoding gene (CcHyPRP) in yeast and Arabidopsis affords multiple abiotic stress tolerance. Plant Biotechnol J, 2010, 8: 76–87
[13]Zhang G Y, Chen M, Li L C, Xu Z S, Chen X P, Guo J M, Ma Y Z. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. J Exp Bot, 2009, 60: 3781–3796
[14]Jose-Estanyol M, Ruiz-Avila L, Puigdomenech P. A maize embryo-speci?c gene encodes a proline-rich and hydrophobic protein. Plant Cell, 1992, 4: 413–423
[15]Jose-Estanyol M, Puigdomenech P. Plant cell wall glycoproteins and their genes. Plant Physiol Biochem, 2000, 38: 97–108
[16]Yamada T, Kuroda K, Jitsuyama Y, Takezawa D, Arakawa K, Fujikawa, S. Roles of the plasma membrane and the cell wall in the responses of plant cells to freezing. Planta, 2002, 215: 770–778
[17]Goodwin W, Pallas J A, Jenkins G I. Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus. Plant Mol Biol, 1995, 27: 411–418
[18]Achuo A E, Audenaert K, Meziane H, Hofte M. The salicylic acid-dependent defence pathway is effective against different pathogens in tomato and tobacco. Plant Pathol, 2004, 53: 65–72
[19]Bleeeker A B, Kende H. Ethylene: a gaseous signal molecule in Plants. Annu Rev Cell Dev Biol, 2000, 16: 1–18
[20]Detelf M, Furini A, Franeesseo S. Structure and regulation of an ABA-and desiccation-responsive gene from the resurrection Plant Cratrostigma Plantagineum. Plant Mol Biol, 1994, 24: 549–560
[21]Vijayan P, Shoekey J, Levesque C A, Cook R J, Browse J. A role for jasmonate in pathogen defense of Arabidopsis. Proc Natl Acad Sci USA, 1998, 95: 7209–7214
[1] Zuo Tong-Hong, Zhang He-Cui, Zeng Jing, Zhu Li-Quan. Molecular cloning and expression analysis of BoPUB3L associated with self-incompatibility in Brasscia oleracea [J]. Acta Agronomica Sinica, 2026, 52(6): 1698-1710.
[2] Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling. Genome-wide association analysis and prediction model construction for soybean plant height [J]. Acta Agronomica Sinica, 2026, 52(6): 1743-1756.
[3] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[4] Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[5] Wang Yi-Han, Li Fu-Chang, Liu Yi, Zhu Guo-Peng. Cloning of the IbOPR2 gene promoter and identification of regulatory factors in sweetpotato [J]. Acta Agronomica Sinica, 2026, 52(4): 1268-1276.
[6] Tian Li-Tao, Ding Ning, Wang Shu-Lin, Qi En-Fang, Zhang Rong, Wang Rui-Rui, Ma Li-Wen, Li Jian-Wu, Yang Jiang-Wei. Genome-wide identification of the Argonaute gene family and its induction by late blight in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2026, 52(4): 1116-1126.
[7] Yang Zong-Tao, Yang Ting, Wang Yu-Tong, Ai Jing, Li Yan-Ye, Liu Jia-Yong, Deng Jun, Zhao Yong, Zhang Yue-Bin. Identification and expression analysis of the CLC gene family in sugarcane [J]. Acta Agronomica Sinica, 2026, 52(3): 722-734.
[8] Meng Cheng, Wang Zhe. Genome-wide identification and expression analysis of the ZmPFK gene family under biotic and abiotic stresses in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 764-779.
[9] Zhang Li-Lan, Yang Jun, Wang Rang-Jian. Identification of candidate genes related to glycoside aroma precursor content in tea plant using WGCNA [J]. Acta Agronomica Sinica, 2026, 52(2): 494-513.
[10] Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[11] WANG Ke-Jing, LI Xiang-Hua. Endangerment assessment of the perennial species G. tabacina and G. tomentella of the genus Glycine Willd. in China [J]. Acta Agronomica Sinica, 2025, 51(8): 2009-2019.
[12] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[13] HE Hong-Li, ZHANG Yu-Han, YANG Jing, CHENG Yun-Qing, ZHAO Yang, LI Xing-Nuo, SI Hong-Liang, ZHANG Xing-Zheng, YANG Xiang-Dong. Creation and physiological analysis of an e1-as gene mutant in soybean [J]. Acta Agronomica Sinica, 2025, 51(8): 2228-2239.
[14] WANG Bin, MENG Jiang-Yu, QIU Hao-Liang, HE Ya-Jun, QIAN Wei. Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(8): 2100-2110.
[15] HU Meng, SHA Dan, ZHANG Sheng-Rui, GU Yong-Zhe, ZHANG Shi-Bi, LI Jing, SUN Jun-Ming, QIU Li-Juan, LI Bin. QTL mapping and candidate gene screening for branch number in soybean [J]. Acta Agronomica Sinica, 2025, 51(7): 1747-1756.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd