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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2152-2157.doi: 10.3724/SP.J.1006.2011.02152

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression of a Stress-induced GmPRP Gene in Soybean (Glycine max)

ZHAI Ying, LEI Ting-Ting,YAN Fan, HUANG Kai-Meng, LI Xiao-Wei, ZHANG Qing-Lin, ZHANG Hai-Jun, SU Lian-Tai, SUN Xin, WANG Ying, LI Jing-Wen*,WANG Qing-Yu*   

  1. College of Plant Sciences, Jilin University, Changchun 130062, China
  • Received:2011-05-10 Revised:2011-07-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 王庆钰, E-mail: wqy414cn@yahoo.com.cn; 李景文, E-mail: ljwk9@163.com

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Abstract: Plant proline-rich proteins (PRPs) are putative cell wall proteins, which are usually associated with different abiotic and biotic stress conditions. A soybean mRNA sequence encoding a proline-rich protein (PRP) was cloned and designated as GmPRP from Jilin 32 immature embryo gene expression profiles using RT-PCR. The GmPRP consisted of an ORF with a length of 396 bp, and encoded 131 amino acids (13.79 kD) with an isoelectric point of 8.96. There was no intron in the DNA sequence of GmPRP. Except a repetitive proline-rich domain, GmPRP also contained a signal peptide in the N-terminal domain and a conserved eight cysteine motif in the C-terminal domain. The amino acid sequences of GmPRP, PvPRP and CcHyPRP shared high homology through phylogenetic analysis. The length of the promoter was 683 bp, containing several stress-induced elements: ABRE-like,G-box,W-box,GT-1,MYB,MYC,BIHD10s,DPBF,SEBF, and WRKY. Real-time quantitative PCR (qPCR) analysis revealed that GmPRP expressed highly in root and leaf and low in flower. qPCR was also performed to investigate the expression profiles of the GmPRP under different stresses such as drought, high salt, low temperature, wound, SA (salicylic acid), ETH (ethane), ABA (abscisic acid) and MeJA (methyl jasmonate). Under these stresses GmPRP showed up-regulated expression patterns. These results revealed that GmPRP might be involved in multiple pathways of plants responding to the different environmental conditions.

Key words: GmPRP, Soybean, Adversity stresses, Promoter, Expression analysis

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