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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2158-2166.doi: 10.3724/SP.J.1006.2011.02158


Analysis of Wheat Leaf Rust Resistance Genes in 14 Wheat Cultivars or Lines

HU Ya-Ya, ZHANG Na, LI Lin-Mao, YANG Wen-Xiang*,LIU Da-Qun*   

  1. Department of Plant Pathology, Agricultural University of Hebei / Biological Control Center of Plant Diseases and Plant Pests of Hebei Province / National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China
  • Received:2011-09-02 Revised:2011-09-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 杨文香, E-mail: wenxiangyang2003@163.com, Tel: 0312-7528585; 刘大群, E-mail: ldq@hebau.edu.cn, Tel: 0312-7528500

Abstract: The objective of this study was to detect resistance genes to leaf rust in 14 wheat cultivars or lines. The resistance of the 14 wheat cultivars or lines was investigated at seedling and adult stages, and the resistance genes at seedling stage were postulated by inoculating 16 races of Puccinia triticina. Further validation was conducted by using 21 molecular markers cosegregated with or closely linked to the known Lr genes. According to the results of both phenotypic identification and molecular marker data, the information of the resistance genes in the 14 cultivars or lines was obtained. Genes Lr3a, Lr28 and Lr50 were postulated in s98351-2-2-2-1. Gene Lr37 was possibly carried by 9629-03A-4-1-1. Lr26 was present in 97167-1-2-1-1-2-1, 919-20-2c2, 9589, Mianzhong 438, 9916-8-6, and 9916-8-18. Lr28 was possibly present in 96104-1-5-1c2. Lr1 was contained in 00-55-3-1-1. Lr24, Lr37, and Lr38 were possibly in 1R13. Lr24 and Lr38 were possibly carried by 1R17. Gene Lr10 and Lr34 were detected in 1R35, and Lr3a and Lr50 were also postulated in 1R35. Besides, 9524-1-2-2-1 might contain unknown or untested resistance genes against P. triticina pathotypes. Genes Lr9, Lr19, Lr20, Lr21, Lr29, Lr35, and Lr42 were not present in all cultivars or lines. The results indicate that these cultivars and lines carry diverse resistance genes to wheat leaf rust, and can be used as resistance resource for resistance breeding.

Key words: Wheat leaf rust, Resistance gene, Gene postulation, Molecular marker-assisted selection

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