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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (12): 2158-2166.doi: 10.3724/SP.J.1006.2011.02158

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Wheat Leaf Rust Resistance Genes in 14 Wheat Cultivars or Lines

HU Ya-Ya, ZHANG Na, LI Lin-Mao, YANG Wen-Xiang*,LIU Da-Qun*   

  1. Department of Plant Pathology, Agricultural University of Hebei / Biological Control Center of Plant Diseases and Plant Pests of Hebei Province / National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China
  • Received:2011-09-02 Revised:2011-09-25 Online:2011-12-12 Published:2011-09-29
  • Contact: 杨文香, E-mail: wenxiangyang2003@163.com, Tel: 0312-7528585; 刘大群, E-mail: ldq@hebau.edu.cn, Tel: 0312-7528500

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Abstract: The objective of this study was to detect resistance genes to leaf rust in 14 wheat cultivars or lines. The resistance of the 14 wheat cultivars or lines was investigated at seedling and adult stages, and the resistance genes at seedling stage were postulated by inoculating 16 races of Puccinia triticina. Further validation was conducted by using 21 molecular markers cosegregated with or closely linked to the known Lr genes. According to the results of both phenotypic identification and molecular marker data, the information of the resistance genes in the 14 cultivars or lines was obtained. Genes Lr3a, Lr28 and Lr50 were postulated in s98351-2-2-2-1. Gene Lr37 was possibly carried by 9629-03A-4-1-1. Lr26 was present in 97167-1-2-1-1-2-1, 919-20-2c2, 9589, Mianzhong 438, 9916-8-6, and 9916-8-18. Lr28 was possibly present in 96104-1-5-1c2. Lr1 was contained in 00-55-3-1-1. Lr24, Lr37, and Lr38 were possibly in 1R13. Lr24 and Lr38 were possibly carried by 1R17. Gene Lr10 and Lr34 were detected in 1R35, and Lr3a and Lr50 were also postulated in 1R35. Besides, 9524-1-2-2-1 might contain unknown or untested resistance genes against P. triticina pathotypes. Genes Lr9, Lr19, Lr20, Lr21, Lr29, Lr35, and Lr42 were not present in all cultivars or lines. The results indicate that these cultivars and lines carry diverse resistance genes to wheat leaf rust, and can be used as resistance resource for resistance breeding.

Key words: Wheat leaf rust, Resistance gene, Gene postulation, Molecular marker-assisted selection

[1]Komer J A. Genetics of resistance to wheat leaf rust. Annu Rev Phytopathol, 1996, 34: 435–455
[2]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2010 supplement.
[2011-03-01]. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2010.pdf
[3]Yang W-X(杨文香). The postulated genes for resistance to leaf rust in 21 wheat cultivars used in Hebei province. J Agric Univ Hebei (河北农业大学学报), 2000, 23(3): 69–72 (in Chinese with English abstract)
[4]Chen W-Q(陈万权), Qin Q-M(秦庆明), Chen Y-L(陈扬林), Wu Z-H(吴支行), Ma Z-Q(马志强), Liao Q(廖琴). Leaf rust resistance of 40 wheat cultivars in China. Acta Phytopathol Sin (植物病理学报), 2008, 38(1): 51–57 (in Chinese with English abstract)
[5]Yang W-X(杨文雄), Yang F-P(杨芳萍), Liang D(梁丹), He Z-H(何中虎), Shang X-W(尚勋武), Xia X-C(夏先春). Molecular characterization of slow-rusting genes Lr34/Yr18 in Chinese wheat cultivars. Acta Agron Sin (作物学报), 2008, 34(7): 1109–1113 (in Chinese with English abstract)
[6]Ding Y-H(丁艳红), Liu H(刘欢), Shi L-H(师丽红), Wen X-L(温晓蕾), Zhang N(张娜), Yang W-X(杨文香), Liu D-Q(刘大群). Wheat leaf rust resistance in 28 Chinese wheat mini-core collections. Acta Agron Sin (作物学报), 2010, 36(7): 1126–1134 (in Chinese with English abstract)
[7]Flor H H. The complementary genetics systems in flax and flax rust. Adv Genet, 1956, 8: 29–54
[8]Browder L E. Designation of two genes for resistance to Puccinia recondita in triticum aestivum. Crop Sci, 1972, 12: 705–706
[9]Yuan Z-Y(原宗英), Chen W-Q(陈万权), Wu Y-P(武英鹏), Li Y-F(李艳芳), Zeng W-F(曾卫芳). Leaf rust resistance of major wheat cultivars in Shanxi Province. Acta Agric Boreali-Sin(华北农学报), 2003, 18(4): 107–110 (in Chinese with English abstract)
[10]Li Z F, Xia X C, He Z H, Li X, Zhang L J, Wang H Y, Meng Q F, Yang W X, Li G Q, Liu D Q. Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis, 2010, 94: 45–53
[11]Qiu J W, Schürch A C, Yahiaoui N, Dong L L, Fan H J, Zhang Z J, Keller B, Ling H Q. Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theor Appl Genet, 2007, 115: 159–168
[12]Gupta S K, Charpe A, Koul S, Prabhu K V, Haq Q M R. Development and validation of molecular markers linked to an Aegilops umbellulata-derived leaf-rust-resistance gene, Lr9, for marker-assisted selection in bread wheat. Genome, 2005, 48: 823–830
[13]Schachermayr G, Siedler H, Gale M D, Winzeler H, Winzeler M, Keller B. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet, 1994, 88: 110–115
[14]Schachermayr G, Feuillet C, Keller B. Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Mol Breed, 1997, 3: 65–74
[15]Gupta S K, Charpe A, Prabhu K V, Haque Q M R. Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theor Appl Genet, 2006, 113: 1027–1036
[16]Neu C, Stein N, Keller B. Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome, 2002, 45: 737–744
[17]Huang L, Gill B S. An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat. Theor Appl Genet, 2001, 103: 1007–1013
[18]Schachermayr G M, Messmer M M, Feuillet C, Winzeler H, Winzeler M, Keller B. Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theor Appl Genet, 1995, 90: 982–990
[19]Gupta S K, Charpe A, Koul S, Haque Q M R, Prabhu K V. Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica, 2006, 150: 233–240
[20]Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL•1RS wheat–rye chromosome translocations. Plant Breed, 2006, 125: 302–304
[21]Froidmont D D. A codominant marker for the 1BL•1RS wheat-rye translocation via multiplex PCR. J Cereal Sci, 1998, 27: 229–232
[22]Cherukuri D P, Gupta S K, Charpe A, Koul S, Prabhu K V, Singh R B, Haq Q M R. Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica, 2005, 143: 19–26
[23]Tar M, Purnhauser L, Csösz L, Mesterházy A, Gyulai G. Identification of molecular markers for an efficient leaf rust resistance gene (Lr29) in wheat. Acta Biol Szegediensis, 2002, 46: 133–134
[24]Lagudah E S, Mcfadden H, Singh R P, Huerta-Espino J, Bariana H S, Spielmeyer W. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet, 2006, 114: 21–30
[25]Lagudah E S, Krattinger S G, Herrera-Foessel S, Singh R. P, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter L L, Keller B. Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet, 2009, 119: 889–898
[26]Gold J, Harder D, Townley-Smith F, Aung T, Procunier J. Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. Plant Biotechnol, 1999, 2: 1–6
[27]Helguera M, Khan I A, Kolmer J, Lijavetzky D, Zhong-Qi L, Dubcovsky J. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci, 2003, 43: 1839–1847
[28]Yan H-F(闫红飞), Yang W-X(杨文香), Chu D(褚栋), Liu D-Q(刘大群). A new marker tagged to the leaf rust resistance gene Lr38. Sci Agric Sin(中国农业科学), 2008, 41(11): 3604–3609 (in Chinese with English abstract)
[29]Sun X C, Bai G H, Carver B F, Bowden R. Molecular mapping of wheat leaf rust resistance gene Lr42. Crop Sci, 2010, 50: 59–66
[30]Helguera M, Khan I A, Dubcovsky J. Development of PCR markers for the wheat leaf rust resistance gene Lr47. Theor Appl Genet, 2000, 100: 1137–1143
[31]Chen Y-F(陈云芳), Liu L(刘莉), Yang W-X(杨文香), Liu D-Q(刘大群). Molecular marker detection of leaf rust resistance gene Lr19 in 33 common wheat cultivars. Mol Plant Breed (分子植物育种), 2008, 6(5): 1015–1018 (in Chinese with English abstract)
[32]Hanzalová A, Sumíková T, Bartoš P. Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech Republic. Czech J Genet Plant Breed, 2009, 45(2): 79–84
[33]Roelfs A P, Singh R P, Saari E E. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, DF: CIMMYT, 1992. pp 7–14
[34]Dubin H J, Johnson R, Stubbs R W. Postulated genes for resistance to strip rust in selected CIMMYT and related wheats. Plant Dis, 1989, 73: 472–475
[35]Gill K S, Lubbers E L, Gill B S, Raupp W J, Cox T S. A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome, 1991, 34: 362–374
[36]Vida G, Gál M, Uhrin A, Veisz O, Syed N H, Flavell A J, Wang Z, Bed? Z. Molecular markers for the identification of resistance genes and marker-assisted selection in breeding wheat for leaf rust resistance. Euphytica, 2009, 170: 67–76
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