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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 408-415.doi: 10.3724/SP.J.1006.2012.00408

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of the Resistant Gene to Rice Blast in the Dual Purpose Genic Male Sterile Rice, LongS

WANG Jian-Long1,2,WU Li-Qun2,LIU Jian-Feng1,DAI Liang-Ying1,LIU Xiong-Lun1,XIAO Ying-Hui1,XIE Hong-Jun3,LIU Qun-En1,LI Ting1,JIA Xian-Yong2,WANG Guo-Liang1,4,*,YUAN Long-Ping5,*   

  1. 1Hunan Agricultural University, Changsha 410128, China; 2Hunan Jinjian Seed Industry Co., Ltd, Changde 415000, China; 3Hunan Rice Research Institute, Changsha 410125, China; 4Ohio State University, Columbus 43210, USA; 5China National Hybrid Rice Research and Development Center, Changsha 410125, China
  • Received:2011-06-21 Revised:2011-12-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 袁隆平, E-mail: lpyuan@hhrrc.ac.cn, Tel: 0731-82872998; 王国梁, E-mail: wang.620@osu.edu, Tel: 0731-84638423

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Abstract: LongS is a dual purpose genic male sterile rice with broad-spectrumresistance to rice blast. The objective of the present study was to identifythe resistance spectrumto rice blast, to analyze thegenetic behavior ofresistance gene, and tomap the major resistancegenes in LongS. LongS had a resistance frequency of 100% inoculatedwith 41strainsofMagnaporthe oryza. Population geneticanalysis showed that theresistance genes in LongSexhibited dominant inheritance, the genetic model of R gene varied depend on the strainsof Magnaporthe oryzae. The main-effect resistant gene to rice blast was fine mapped, by using the bulk segregant analysis (BSA) and recessive class analysis (RCA) methods, with the F2 population derived from the resistant parent of LongS and the susceptible parent of Nipponbare. Asingle resistant gene to the race of 318-2 locatedon the interval flanked by the SSRmarkersof M1 and M2with a genetic distance of 1.3 cM onchromosome 9 were adjacent to the broad-spectrum blast resistance gene, Pi5. Both of the resistance spectrum and resistant frequency of LongS, however, were significantly different to those of resistant gene of Pi5 and Pii. In conclusion, the major-effect resistant gene identified in this study may be a new broad-spectrum blast resistance gene. The DNA markers linked to the new R gene identified in this study should be useful for marker-aided breeding of blast-resistant rice cultivars.

Key words: Rice, LongS, Rice blast, Resistant gene, Gene mapping

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