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Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (07): 1210-1213.

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Molecular Identification and Electron Microscopy Detection for Pathogen of Sugarcane Yellow Leaf Syndrome

GAO San-Ji,GUO Jin-Long,CHEN Ru-Kai*,HUANG Xiao-Di,XU Jing-Sheng,WANG Qin-Nan   

  1. Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
  • Received:2006-10-08 Revised:1900-01-01 Online:2007-07-12 Published:2007-07-12
  • Contact: CHEN Ru-Kai

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Abstract:

Causal organism of sugarcane yellow leaf syndrome (YLS) was identified by electron micrographs, RT-PCR and tissue blot immunoassay (TBIA) in Fuzhou, China. Virus particles like sugarcane yellow leaf virus (SCYLV) were found in phloem companion cells from plant with YLS symptoms by electron micrographs. A 556 bp fragment was amplified by RT-PCR. The sequences of cDNA and its amino acids suggested that the fragment was a part of gene of coat protein that shared above 95% homology with SCYLV isolates from other countries. Positive reaction appeared as dark purple spot at the location of the phloem cells by TBIA. The above results suggested that pathogen of sugarcane yellow leaf syndrome is SCYLV in Fuzhou.

Key words: Sugarcane, Yellow leaf syndrome, Sugarcane yellow leaf virus, Molecular identification

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