甘蔗割手密种转录因子NAP亚家族的鉴定及SsNAP2a参与叶片衰老的功能分析

doi:10.3724/SP.J.1006.2024.34037

Abstract

Abstract:

NAP (NAC-like, Activated by APETALA3/PISTILLATA) is a subfamily of the transcription factor NAC gene family, which is widely involved in regulating plant growth and development, leaf senescence, and response to hormones and abiotic stress. Firstly, the NAP subfamily members were identified from the genomic database of Saccharum spontaneum, and phylogenetic analysis, conserved domains, and cis-regulatory elements were predicted using comparative genomics and various bioinformatics methods. Secondly, the allele SsNAP2a of the SsNAP2 member was isolated from the cDNA library of a wide accession SES208. The relative expression characteristics of the SsNAP2a were detected by qRT-PCR under hormone and abiotic stresses at different growth and development stages. Finally, transient overexpression and subcellular localization performed the function of SsNAP2a gene. The results showed that five NAP subfamily members were identified in the genome of S. spontaneum. The subcellular localization predicted that the encoded proteins of all members were localized in the nucleus. The K_a/K_s ratio of five gene pairs was less than 1, indicating that purifying selection was crucial in the evolution. Phylogenetic analysis revealed that 46 NAP members, including five representative angiosperms (Arabidopsis thaliana, Ananas comosus, Oryza sativa, Zea mays, and Sorghum bicolor), 12 reported species, and the S. spontaneum, were classified into four Clades. The evolution order was Clade I > Clade II > Clade III > Clade IV. In addition, the promoter regions of SsNAP members predicted many cis-acting elements in response to abscisic acid, jasmonic acid, low temperature, and other stresses. We speculated that they were involved in various hormone and abiotic stress-related response pathways. Furthermore, the full-length cDNA sequence of the SsNAP2a gene (GenBank accession number: OQ335094) was isolated from the wild accession SES208, with an open reading frame of 1173 bp and encoding 390 amino acid residues. The amino acid sequence similarity between SsNAP2 and SsNAP2a proteins was 97.70%. There was a difference of 10 amino acid residues, indicating that the autopolyploid allelic sequences of Saccharum species were significant difference. The qRT-PCR demonstrated that the SsNAP2a gene was constitutively expressed in various tissues of S. spontaneum, especially in the senescent bark and root, and its expression level was significantly induced under the treatment of ethylene, ET, abscisic acid (ABA), low temperature at 4℃, and high temperature at 40℃. However, the relative expression level of the SsNAP2a gene was significantly down-regulated under 8% polyethylene glycol (PEG) stress. Subcellular localization revealed that the SsNAP2a-GFP fusion protein was located in the cell nucleus of Nicotiana benthamiana leaves. After transient overexpression of the SsNAP2a gene for seven days, the leaves of N. benthamiana displayed obvious curling and shriveling phenotype. The relative expression level of ET synthesis-related genes (NtEFE26, NtAccdeaminase) was significantly up-regulated, while salicylic acid, jasmonic acid, and ABA synthesis-related genes (NtPR-1a/c, NtPR3, and NtAREB1) were significantly down-regulated, indicating that the SsNAP2a gene was involved in multiple hormone signaling pathways to induce leaf senescence. These results lay a foundation for exploring the biological functions of NAP subfamily gene members involved in sugarcane leaf senescence and provide candidate gene resources for breeding anti-senescence new cultivars.

Key words: Saccharum spontaneum, NAP gene, leaf senescence, hormone stress, functional analysis, sugarcane

WANG Heng-Bo, FENG Chun-Yan, ZHANG Yi-Xing, XIE Wan-Jie, DU Cui-Cui, WU Ming-Xing, ZHANG Ji-Sen. Genome-wide identification of NAP transcription factors subfamily in Saccharum spontaneum and functional analysis of SsNAP2a involvement in leaf senescence[J].Acta Agronomica Sinica, 2024, 50(1): 110-125.

Figures/Tables 11

Table 1

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

References 49

[1]	李伟, 韩蕾, 钱永强, 孙振元. 植物NAC转录因子的种类、特征及功能. 应用与环境生物学报, 2011, 17: 596-606.
	Li W, Han L, Qian Y Q, Sun Z Y. Characteristics and functions of NAC transcription factors in plants. Chin J Appl Environ Biol, 2011, 17: 596-606. (in Chinese with English abstract)
[2]	Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res, 2003, 10: 239-247.
[3]	Sablowski R W, Meyerowitz E M. A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA. Cell, 1998, 92: 93-103. doi: 10.1016/s0092-8674(00)80902-2 pmid: 9489703
[4]	Guo Y, Gan S. AtNAP, a NAC family transcription factor, has an important role in leaf senescence. Plant J, 2006, 46: 601-612.
[5]	Ernst H A, Olsen A N, Larsen S, Lo Leggio L. Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Rep, 2004, 5: 297-303. doi: 10.1038/sj.embor.7400093 pmid: 15083810
[6]	范凯, 王学德, 袁淑娜, 王铭. 植物转录因子NAP亚家族的研究进展. 中国农业科学, 2014, 47: 209-220. doi: 10.3864/j.issn.0578-1752.2014.02.001
	Fan K, Wang X D, Yuan S N, Wang M. Recent advances in research of transcription factor nap subfamily in plants. Sci Agric Sin, 2014, 47: 209-220. (in Chinese with English abstract) doi: 10.3864/j.issn.0578-1752.2014.02.001
[7]	Olsen A N, Ernst H A, Leggio L L, Skriver K. NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci, 2005, 10: 79-87. doi: 10.1016/j.tplants.2004.12.010 pmid: 15708345
[8]	Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell, 1997, 9: 841-857. doi: 10.1105/tpc.9.6.841 pmid: 9212461
[9]	范凯. 植物转录因子NAP的系统发育及其对叶片衰老的调控. 浙江大学博士学位论文, 浙江杭州, 2015.
	Fan K. Phyogenetic Analysis of the NAP Transcription Factor in Plants and Its Regulation to Leaf Senescence. PhD Dissertation of Zhejiang University, Hangzhou, Zhejiang, China, 2015. (in Chinese with English abstract)
[10]	Kalivas A, Pasentsis K, Argiriou A, Tsaftaris A S. Isolation, characterization, and expression analysis of an NAP-like cDNA from Crocus (Crocus sativus L.). Plant Mol Biol Rep, 2010, 28: 654-663. doi: 10.1007/s11105-010-0197-x
[11]	Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science, 2006, 314: 1298-1301. doi: 10.1126/science.1133649 pmid: 17124321
[12]	Meng Q, Zhang C, Gai J, Yu D. Molecular cloning, sequence characterization and tissue-specific expression of six NAC-like genes in soybean (Glycine max (L.) Merr.). J Plant Physiol, 2007, 164: 1002-1012. doi: 10.1016/j.jplph.2006.05.019
[13]	Fernandez L, Ageorges A, Torregrosa L. A putative NAP homolog specifically expressed during grapevine flower and berry development. Vitis, 2006, 45, 51-52.
[14]	Chai L, Biswas M K, Ge X, Deng X. Isolation, characterization, and expression analysis of an SKP1-like gene from ‘Shatian’ Pummelo. Plant Mol Biol Rep, 2010, 28: 569-577. doi: 10.1007/s11105-010-0184-2
[15]	樊金萍, 赵然. 金鱼藤中ApNAP基因的克隆和表达模式分析. 植物科学学报, 2014, 32: 251-258.
	Fan J P, Zhao R. Cloning and expression pattern analysis of the ApNAP gene in Asarina procumbens. Plant Sci J, 2014, 32: 251-258. (in Chinese with English abstract)
[16]	晁跃辉, 李珊珊, 滕珂, 许立新, 肖国增, 郭涛, 韩烈保. 紫花苜蓿MsNAP基因克隆及烟草转化. 中国草地学报, 2016, 38(2): 13-19.
	Chao Y H, Li S S, Teng K, Xu L X, Xiao G Z, Guo T, Han L B. Cloning of MsNAP gene from Medicago sativa and transformation to tobacco (Nicotiana tabacum). Chin J Grassland, 2016, 38(2): 13-19. (in Chinese with English abstract)
[17]	Huang G Q, Li W, Zhou W, Zhang J M, Li D D, Gong S Y, Li X B. Seven cotton genes encoding putative NAC domain proteins are preferentially expressed in roots and in responses to abiotic stress during root development. Plant Growth Regul, 2013, 71: 101-112. doi: 10.1007/s10725-013-9811-x
[18]	Liang C, Wang Y, Zhu Y, Tang J, Hu B, Liu L, Ou S, Wu H, Sun X, Chu J, Chu C. OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice. Proc Natl Acad Sci USA, 2014, 111: 10013-10018. doi: 10.1073/pnas.1321568111 pmid: 24951508
[19]	Zhou Y, Huang W, Liu L, Chen T, Zhou F, Lin Y. Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence. BMC Plant Biol, 2013, 13: 132. doi: 10.1186/1471-2229-13-132
[20]	Carrillo-Bermejo E A, Gamboa-Tuz S D, Pereira-Santana A, Keb-Llanes M A, Castaño E, Figueroa-Yañez L J, Rodriguez-Zapata L C. The SoNAP gene from sugarcane (Saccharum officinarum) encodes a senescence-associated NAC transcription factor involved in response to osmotic and salt stress. J Plant Res, 2020, 133: 897-909. doi: 10.1007/s10265-020-01230-y pmid: 33094397
[21]	Oz M T, Altpeter A, Karan R, Merotto A, Altpeter F. CRISPR/Cas9-mediated multi-allelic gene targeting in sugarcane confers herbicide tolerance. Front Genome Ed, 2021, 3: 673566. doi: 10.3389/fgeed.2021.673566
[22]	许孚, 汪洲涛, 路贵龙, 阙友雄. 甘蔗遗传改良中的基因工程: 适用、成就、局限和展望. 农业生物技术学报, 2022, 30: 580-593.
	Xu F, Wang Z T, Lu G L, Que Y X. Genetic engineering in sugarcane improvement: adaptability, achievements, limitations and prospects. J Agric Biotechnol, 2022, 30: 580-593. (in Chinese with English abstract)
[23]	王恒波, 张畅, 吴明星, 李湘, 蒋钟莉, 林容潇, 郭晋隆, 阙友雄. 甘蔗割手密种NAC转录因子ATAF亚家族鉴定及栽培品种ScNAC2 基因的功能分析. 作物学报, 2023, 49: 46-61. doi: 10.3724/SP.J.1006.2023.24005
	Wang H B, Zhang C, Wu M X, Li X, Jiang Z L, Lin R X, Guo J L, Que Y X. Genome-wide identification of NAC transcription factors ATAF subfamily in Saccharum spontaneum and functional analysis of its homologous gene ScNAC2 in sugarcane cultivar. Acta Agron Sin, 2023, 49: 46-61 (in Chinese with English abstract).
[24]	张旭, 凌辉, 刘峰, 黄宁, 王玲, 毛花英, 李聪娜, 汤翰臣, 苏炜华, 苏亚春, 阙友雄. 一个甘蔗IId类WRKY转录因子基因的克隆和表达分析. 中国农业科学, 2018, 51: 4409-4423. doi: 10.3864/j.issn.0578-1752.2018.23.002
	Zhang X, Ling H, Liu F, Huang N, Wang L, Mao H Y, Li C N, Tang H C, Su W H, Su Y C, Que Y X. Cloning and expression analysis of a IId sub-group WRKY transcription factor gene from sugarcane. Sci Agric Sin, 2018, 51: 4409-4423. (in Chinese with English abstract)
[25]	杜翠翠, 吴明星, 张雅婷, 谢婉婕, 张积森, 王恒波. 甘蔗割手密种糖转运蛋白基因SsSWEET11的克隆与功能分析. 作物学报, 2023, 51: 1-12.
	Du C C, Wu M X, Zhang Y T, Xie W J, Zhang J S, Wang H B. Cloning and functional analysis of sucrose transporter protein SsSWEET11 gene in sugarcane (Saccharum spontaneum L.). Acta Agron Sin, 2023, 51: 1-12. (in Chinese with English abstract)
[26]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant, 2020, 13: 1194-1202. doi: S1674-2052(20)30187-8 pmid: 32585190
[27]	苏亚春, 李聪娜, 苏炜华, 尤垂淮, 岑光莉, 张畅, 任永娟, 阙友雄. 甘蔗割手密种类甜蛋白家族鉴定及栽培种同源基因功能分析. 作物学报, 2021, 47: 1275-1296. doi: 10.3724/SP.J.1006.2021.04192
	Su Y C, Li C N, Su W H, You C H, Cen G L, Zhang C, Ren Y J, Que Y X. Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar. Acta Agron Sin, 2021, 47: 1275-1296. (in Chinese with English abstract)
[28]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001, 25: 402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[29]	Liu F, Huang N, Wang L, Ling H, Sun T, Ahmad W, Muhammad K, Guo J, Xu L, Gao S, Que Y, Su Y. A novel L-ascorbate peroxidase 6 gene, ScAPX6, plays an important role in the regulation of response to biotic and abiotic stresses in sugarcane. Front Plant Sci, 2017, 8: 2262. doi: 10.3389/fpls.2017.02262
[30]	申娟娟. 本氏烟响应黑暗胁迫的多组学分析. 郑州大学硕士学位论文. 河南郑州, 2021.
	Shen J J. Muti-Omics Analysis of Nicotiana bethamiana in response to Dark Stress. MS Thesis of Zhengzhou University, Zhengzhou, Henan, China, 2021 (in Chinese with English abstract).
[31]	Brogue K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais C J, Broglie R. Transgenic plants with enhanced resistance to the fungal pathogen. Science, 1991, 254: 1194-1197. doi: 10.1126/science.254.5035.1194 pmid: 17776411
[32]	李钰卓, 刘柯, 袁璐, 曹丽雯, 王挺进, 甘苏生, 陈利萍. 菜薹BrNAP克隆及其在采后叶片衰老中的功能分析. 园艺学报, 2021, 48(1): 60-72. doi: 10.16420/j.issn.0513-353x.2020-0295
	Li Y Z, Liu K, Yuan L, Cao L W, Wang T J, Gan S S, Chen L P. Cloning and functional analyses of BrNAP in postharvest leaf senescence in Chinese flowering cabbage. Acta Hortic Sin, 2021, 48(1): 60-72. (in Chinese with English abstract)
[33]	Chen N, Goodwin P H, Hsiang T. The role of ethylene during the infection of Nicotiana tabacum by Colletotrichum destructivum. J Exp Bot, 2003, 54: 2449-2456. pmid: 14565949
[34]	朱丽萍, 于壮, 邹翠霞, 李秋莉. 植物逆境相关启动子及功能. 遗传, 2010, 32: 229-234.
	Zhu L P, Yu Z, Zou C X, Li Q L. Plant stress-inducible promoters and their function. Hereditas, 2010, 32: 229-234. (in Chinese with English abstract)
[35]	李钰卓. 菜心BrNAP基因的克隆及其在采后叶片衰老中的功能研究. 浙江大学硕士学位论文, 浙江杭州, 2020.
	Li J Z. Cloning and Functional Analyses of BrNAP in Postharvest Leaf Senescence in Chinese Flowering Cabbage. MS Thesis of Zhejiang University, Hangzhou, Zhejiang, China, 2020. (in Chinese with English abstract)
[36]	He Y, Tang W, Swain J, Green A, Jack T, Gan S. Networking senescence-regulating pathways by using Arabidopsis enhancer trap lines. Plant Physiol, 2001, 126: 707-716. pmid: 11402199
[37]	Ming R, VanBuren R, Wai C M, Tang H, Schatz M C, Bowers J E, Lyons E, Wang M L, Chen J, Biggers E, Zhang J, Huang L, Zhang L, Miao W, Zhang J, Ye Z, Miao C, Lin Z, Wang H, Zhou H, Yim W C, Priest H D, Zheng C, Woodhouse M, Edger P P, Guyot R, Guo H B, Guo H, Zheng G, Singh R, Sharma A, Min X, Zheng Y, Lee H, Gurtowski J, Sedlazeck F J, Harkess A, McKain M R, Liao Z, Fang J, Liu J, Zhang X, Zhang Q, Hu W, Qin Y, Wang K, Chen L Y, Shirley N, Lin Y R, Liu L Y, Hernandez A G, Wright C L, Bulone V, Tuskan G A, Heath K, Zee F, Moore P H, Sunkar R, Leebens-Mack J H, Mockler T, Bennetzen J L, Freeling M, Sankoff D, Paterson A H, Zhu X, Yang X, Smith J A C, Cushman J C, Paull R E, Yu Q. The pineapple genome and the evolution of CAM photosynthesis. Nat Genet, 2015, 47: 1435-1442. doi: 10.1038/ng.3435 pmid: 26523774
[38]	李捷. 水稻NAP转录因子在非生物胁迫调控中的功能分析. 上海师范大学硕士学位论文, 上海, 2013.
	Li J. Characterization of NAC Family Transcription Factor OsNAP Conferring Abiotic Stress in Rice. MS Thesis of Shanghai Normal University, Shanghai, China, 2013. (in Chinese with English abstract)
[39]	Zhang J, Zhang X, Tang H, Zhang Q, Hua X, Ma X, Zhu F, Jones T, Zhu X, Bowers J, Wai C M, Zheng C, Shi Y, Chen S, Xu X, Yue J, Nelson D R, Huang L, Li Z, Xu H, Zhou D, Wang Y, Hu W, Lin J, Deng Y, Pandey N, Mancini M, Zerpa D, Nguyen J K, Wang L, Yu L, Xin Y, Ge L, Arro J, Han J O, Chakrabarty S, Pushko M, Zhang W, Ma Y, Ma P, Lyu M, Chen F, Zheng G, Xu J, Yang Z, Deng F, Chen X, Liao Z, Zhang X, Lin Z, Lin H, Yan H, Kuang Z, Zhong W, Liang P, Wang G, Yuan Y, Shi J, Hou J, Lin J, Jin J, Cao P, Shen Q, Jiang Q, Zhou P, Ma Y, Zhang X, Xu R, Liu J, Zhou Y, Jia H, Ma Q, Qi R, Zhang Z, Fang J, Fang H, Song J, Wang M, Dong G, Wang G, Chen Z, Ma T, Liu H, Dhungana S R, Huss S E, Yang X, Sharma A, Trujillo J H, Martinez M C, Hudson M, Riascos J J, Schuler M, Chen L Q, Braun D M, Li L, Yu Q, Wang J, Wang K, Schatz M C, Heckerman D, Van Sluys M A, Souza G M, Moore P H, Sankoff D, Van Buren R, Paterson A H, Nagai C, Ming R. Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Nat Genet, 2018, 50: 1565-1573. doi: 10.1038/s41588-018-0237-2
[40]	D’Hont A, Ison D, Alix K, Roux C, Glaszmann J C. Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome, 1998, 41: 221-225. doi: 10.1139/g98-023
[41]	Zhang Q, Qi Y, Pan H, Tang H, Wang G, Hua X, Wang Y, Lin L, Li Z, Li Y, Yu F, Yu Z, Huang Y, Wang T, Ma P, Dou M, Sun Z, Wang Y, Wang H, Zhang X, Yao W, Wang Y, Liu X, Wang M, Wang J, Deng Z, Xu J, Yang Q, Liu Z, Chen B, Zhang M, Ming R, Zhang J. Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum. Nat Genet, 2022, 54: 885-896. doi: 10.1038/s41588-022-01084-1 pmid: 35654976
[42]	Torregrosa L. A putative NAP homolog specifically expressed during grapevine flower and berry development. Vitis, 2005, 45: 51-52.
[43]	Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X. A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum. J Exp Bot, 2015, 66: 4669-4682.
[44]	Wang Z, Dane F. NAC (NAM/ATAF/CUC) transcription factors in different stresses and their signaling pathway. Acta Physiol Plant, 2013, 35: 1397-1408. doi: 10.1007/s11738-012-1195-4
[45]	Lu P L, Chen N Z, An R, Su Z, Qi B S, Ren F, Chen J, Wang X C. A novel drought-inducible gene, ATAF1, encodes a NAC family protein that negatively regulates the expression of stress-responsive genes in Arabidopsis. Plant Mol Biol, 2007, 63: 289-305. doi: 10.1007/s11103-006-9089-8
[46]	Peng H, Cheng H Y, Chen C, Yu X W, Yang J N, Gao W R, Shi Q H, Zhang H, Li J G, Ma H. A NAC transcription factor gene of chickpea (Cicer arietinum), CarNAC3, is involved in drought stress response and various developmental processes. J Plant Physiol, 2009, 166: 1934-1945. doi: 10.1016/j.jplph.2009.05.013
[47]	Li D M, Wang J H, Peng S L, Zhu G F, Lyu F B. Molecular cloning and characterization of two novel NAC genes from Mikania micrantha (Asteraceae). Genet Mol Res, 2012, 11: 4383-4401. doi: 10.4238/2012.September.19.3 pmid: 23079980
[48]	Zhang K, Xia X, Zhang Y, Gan S S. An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis. Plant J, 2012, 69: 667-678. doi: 10.1111/tpj.2012.69.issue-4
[49]	Zhang K, Gan S S. An abscisic acid-AtNAP transcription factor- SAG113 protein phosphatase 2C regulatory chain for controlling dehydration in senescing Arabidopsis leaves. Plant Physiol, 2012, 158: 961-969. doi: 10.1104/pp.111.190876

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Li Shaoqing, Li Yangsheng, Wu Fushun, Liao Jianglin, Li Damo. Optimum Fertilization and Its Corresponding Mechanism under Complete Submergence at Booting Stage in Rice[J]. Acta Agronomica Sinica, 2002, 28(01): 115 -120 .
[2]	Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[3]	YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[4]	Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[5]	Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[6]	WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[7]	TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[8]	HU Xi-Yuan;LI Jian-Ping;SONG Xi-Fang. Efficiency of Spatial Statistical Analysis in Superior Genotype Selection of Plant Breeding[J]. Acta Agron Sin, 2008, 34(03): 412 -417 .
[9]	WANG Yan;QIU Li-Ming;XIE Wen-Juan;HUANG Wei;YE Feng;ZHANG Fu-Chun;MA Ji. Cold Tolerance of Transgenic Tobacco Carrying Gene Encoding Insect Antifreeze Protein[J]. Acta Agron Sin, 2008, 34(03): 397 -402 .
[10]	ZHENG Xi;WU Jian-Guo;LOU Xiang-Yang;XU Hai-Ming;SHI Chun-Hai. Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments[J]. Acta Agron Sin, 2008, 34(03): 369 -375 .

名称 Primer name	引物序列 Primer sequences (5′-3′)	备注 Annotation
SsNAP2a-F	ATGACGACGATGATGTCGGCG	基因克隆 Gene cloning
SsNAP2a-R	CTAGAAATGAGGAAGCATGTGATGG	基因克隆 Gene cloning
qRT-PCR-SsNAP2a-F	CCTCCAAGCTCAGGAACGTC	定量PCR引物 qRT-PCR primers
qRT-PCR-SsNAP2a-R	CTCGTAGTCGGCGAGCG	定量PCR引物 qRT-PCR primers
qRT-PCR-SsUBC-F	GATTCTACTGCGGATGGATTGA	甘蔗内参基因 Reference gene in sugarcane
qRT-PCR-SsUBC-R	CAACTTTGGGATGCTTGATACAC	甘蔗内参基因 Reference gene in sugarcane
pMDC202-SsNAP2a-F	CTCGACTCTAGAACTAGTATGACGACGATGATGTCGGCG	过表达载体构建 Construction of overexpression vector
pMDC202-SsNAP2a-R	ATTTTTTCTACCGGTACCCTAGAAATGAGGAAGCATGTGATGG	过表达载体构建 Construction of overexpression vector
pSuper-1300-SsNAP2a-F	GGGGCCCGGGGTCGACATGACGACGATGATGTCGGCG	亚细胞定位载体构建 Construction of subcellular localization vector
pSuper-1300-SsNAP2a-R	CCCTTGCTCACCATGGTACCGAAATGAGGAAGCATGTGATGG	亚细胞定位载体构建 Construction of subcellular localization vector
qPCR-AREB1-ABA-F	ATCCAGAAAAACAGAAAAGAGTGAT	脱落酸通路相关基因 Abscisic acid pathway related gene
qPCR-AREB1-ABA-R	CAACACTACTTCCACCCTCCC	脱落酸通路相关基因 Abscisic acid pathway related gene

名称 Name	基因编号 Sequence ID	氨基酸残基数 Number of amino acid	相对分子量 Molecular weight (kD)	理论等电点 Theoretical pI	不稳定系数 Instability index	平均疏水性 Grand average of hydropathicity	高粱直系同源基因 Orthologous gene from sorghum	非同义和同义替换率 K_a/K_s
SsNAP1	Npp.01C032290.1	371	40.285	9.04	41.98	-0.50	Sobic.001G385800.1	0.46
SsNAP2	Npp.02D037500.1	392	41.876	7.29	44.69	-0.26	Sobic.002G420700.1	0.65
SsNAP3	Npp.03B008200.1	340	36.933	6.33	55.52	-0.45	Sobic.003G105800.1	0.48
SsNAP4	Npp.05B003240.1	396	43.899	6.22	49.34	-0.65	Sobic.005G018500.1	0.34
SsNAP5	Npp.08D002510.1	409	44.912	6.06	51.39	-0.61	Sobic.008G021800.1	0.30

Genome-wide identification of NAP transcription factors subfamily in Saccharum spontaneum and functional analysis of SsNAP2a involvement in leaf senescence

RichHTML419

PDF

Abstract

Cite this article

share this article

Figures/Tables 11

References 49

Related Articles 15

Metrics

Comments

Recommended 10

[1]	TIAN Chun-Yan, BIAN Xin, LANG Rong-Bin, YU Hua-Xian, TAO Lian-An, AN Ru-Dong, DONG Li-Hua, ZHANG Yu, JING Yan-Fen. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane #br# [J]. Acta Agronomica Sinica, 2024, 50(2): 310-324.
[2]	DU Cui-Cui, WU Ming-Xing, ZHANG Ya-Ting, XIE Wan-Jie, ZHANG Ji-Sen, WANG Heng-Bo. Cloning and functional analysis of sucrose transporter protein SsSWEET11 gene in sugarcane (Saccharum spontaneum L.) [J]. Acta Agronomica Sinica, 2023, 49(9): 2385-2397.
[3]	MO Guang-Ling, YU Chen-Jing, LIANG Yan-Lan, ZHOU Ding-Gang, LUO Jun, WANG Mo, QUE You-Xiong, HUANG Ning, LING Hui. RT-PCR cloning and functional analysis of ScbHLH13 in sugarcane [J]. Acta Agronomica Sinica, 2023, 49(9): 2485-2497.
[4]	HU Xin, LUO Zheng-Ying, LI Chun-Jia, WU Zhuan-Di, LI Xu-Juan, LIU Xin-Long. Comparative transcriptome analysis of elite ‘ROC’ sugarcane parents for exploring genes involved in Sporisorium scitamineum infection by using Illumina- and SMRT-based RNA-seq [J]. Acta Agronomica Sinica, 2023, 49(9): 2412-2432.
[5]	YU Quan-Xin, YANG Zong-Tao, ZHANG Hai, CHENG Guang-Yuan, ZHOU Ying-Shuan, JIAO Wen-Di, ZENG Kang, LUO Ting-Xu, HUANG Guo-Qiang, ZHANG Mu-Qing, XU Jing-Sheng. Interaction of sugarcane VAMP associated protein ScPVA12 with SCMV P3N-PIPO [J]. Acta Agronomica Sinica, 2023, 49(9): 2472-2484.
[6]	DING Hong-Yan, FENG Xiao-Xi, WANG Bai-Yu, ZHANG Ji-Sen. Evolution and relative expression pattern of LRRII-RLK gene family in sugarcane Saccharum spontaneum [J]. Acta Agronomica Sinica, 2023, 49(7): 1769-1784.
[7]	ZHANG Zhen-Bo, JIA Chun-Lan, REN Bai-Zhao, LIU Peng, ZHAO Bin, ZHANG Ji-Wang. Effects of combined application of nitrogen and phosphorus on yield and leaf senescence physiological characteristics in summer maize [J]. Acta Agronomica Sinica, 2023, 49(6): 1616-1629.
[8]	WANG Jin-Song, BAI Ge, ZHANG Yan-Hui, SHEN Tian-Yu, DONG Er-Wei, JIAO Xiao-Yan. Impacts of long-term fertilization on post-anthesis leaf senescence, antioxidant enzyme activities and yield in sorghum [J]. Acta Agronomica Sinica, 2023, 49(3): 845-855.
[9]	PAN Jie-Ming, TIAN Shao-Rui, LIANG Yan-Lan, ZHU Yu-Lin, ZHOU Ding-Gang, QUE You-Xiong, LING Hui, HUANG Ning. Identification and expression analysis of PIN-LIKES gene family in sugarcane [J]. Acta Agronomica Sinica, 2023, 49(2): 414-425.
[10]	XIAO Jian, WEI Xing-Xuan, YANG Shang-Dong, LU Wen, TAN Hong-Wei. Effects of intercropping with watermelons on cane yields, soil physicochemical properties and micro-ecology in rhizospheres of sugarcanes [J]. Acta Agronomica Sinica, 2023, 49(2): 526-538.
[11]	YANG Zong-Tao, JIAO Wen-Di, ZHANG Hai, ZHANG Ke-Ming, CHENG Guang-Yuan, LUO Ting-Xu, ZENG Kang, ZHOU Ying-Shuan, XU Jing-Sheng. Interaction of sugarcane glutathione S-transferase ScGSTF1 with P3N-PIPO in response to SCMV infection [J]. Acta Agronomica Sinica, 2023, 49(10): 2665-2676.
[12]	SHEN Qing-Qing, WANG Tian-Ju, WANG Jun-Gang, ZHANG Shu-Zhen, ZHAO Xue-Ting, HE Li-Lian, LI Fu-Sheng. Functional identification of Saccharum spontaneum transcription factor SsWRKY1 to improve drought tolerance in sugarcane [J]. Acta Agronomica Sinica, 2023, 49(10): 2654-2664.
[13]	WANG Heng-Bo, ZHANG Chang, WU Ming-Xing, LI Xiang, JIANG Zhong-Li, LIN Rong-Xiao, GUO Jin-Long, QUE You-Xiong. Genome-wide identification of NAC transcription factors ATAF subfamily in Sacchrum spontaneum and functional analysis of its homologous gene ScNAC2 in sugarcane cultivar [J]. Acta Agronomica Sinica, 2023, 49(1): 46-61.
[14]	LI Pei-Ting, ZHAO Zhen-Li, HUANG Chao-Hua, HUANG Guo-Qiang, XU Liang-Nian, DENG Zu-Hu, ZHANG Yu, ZHAO Xin-Wang. Analysis of drought responsive regulatory network in sugarcane based on transcriptome and WGCNA [J]. Acta Agronomica Sinica, 2022, 48(7): 1583-1600.
[15]	LI Xu-Juan, LI Chun-Jia, WU Zhuan-Di, TIAN Chun-Yan, HU Xin, QIU Li-Hang, WU Jian-Ming, LIU Xin-Long. Expression characteristic and gene diversity analysis of ScHTD2 in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(7): 1601-1613.