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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1196-1204.doi: 10.3724/SP.J.1006.2012.01196

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Relationship of Leaf Shape, Pod Traits and Association with SSR Markers in Soybean Germplasm

WU Bao-Duo**,CHEN Hai-Feng**,GUO Dan-Dan,SHA Ai-Hua,SHAN Zhi-Hui,ZHANG Xiao-Juan,YANG Zhong-Lu,QIU De-Zhen,CHEN Shui-Lian,ZHU Xiao-Ling,ZHANG Chan-Juan,ZHOU Rong*,ZHOU Xin-An*   

  1. Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan 430062, China
  • Received:2011-10-17 Revised:2012-02-22 Online:2012-07-12 Published:2012-05-11
  • Contact: 周蓉, E-mail: zhourong@oilcrops.cn, Tel: 027-86735887; 周新安, E-mail: xazhou@public.wh.hb.cn, Tel: 027-86711563

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Abstract: Pod traits are important factors affecting soybean yield, and they have been identified to tightly associate with leaf shape. In present study, leaf shape/pod traits were analyzed using 167 soybean accessions, which were classified into two types (broad and narrow leaflets) based on the ratio of leaf length/width. Correlation analysis was carried out between leaf shape related traits and pod traits; ANOVA analysis was also performed using 65 SSR markers, evenly distributed in 20 linkage groups; association analysis was carried out using 13 SSR markers on the target regions. Marker 20-285, locating on chromosome I, highly associated with number of seeds per pod, indicating that the gene controlling number of seeds per pod may co-locate with marker 20-285. Almost all markers between 20-26 and 20-45 were associated with ratio of leaf length/width. We speculated that the genes controlling leaf shape were located in the region from marker 20-26 to 20-45. Hence, it was proved the target region harboring candidate genes of leaf shape and pod number was narrowed. Our study may provide a potential approach in molecular marker-assisted breeding of seeds per pod, and a theoretical guide for cloning the gene of seeds per pod.

Key words: Soybean [Glycine max (L.) Merri], Leaf shape, Pod traits, Correlation analysis, Association analysis

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