Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1205-1211.doi: 10.3724/SP.J.1006.2012.01205

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity of High-Molecular-Weight Glutenin Subunit Composition in Chinese Wheat Landraces

XU Xin1,2,**,LI Xiao-Jun1,3,**,ZHANG Ling-Li1,4,LI Xiu-Quan1,YANG Xin-Ming1,LI Li-Hui1,*   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081, China; 2Department of Life Sciences and Technology, Xinxiang University, Xinxiang 453003, China; 3School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China; 4College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
  • Received:2011-11-24 Revised:2012-02-23 Online:2012-07-12 Published:2012-05-11
  • Contact: 李立会, E-mail: lilihui@caas.net.cn, Tel: 010-62186670 ** 同等贡献(Contributed equally to this work)

PDF

1735

Cited

22

Abstract: The high-molecular-weight glutenin subunit (HMW-GS) composition of 76 representative accessions of wheat landraces, collected from nine agro-ecological zones in China, were examined by sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis (SDS-PAGE). The correlation between diversity indexes at Glu-1 locus and altitude, mean annual precipitation, or mean annual temperature was also analyzed. The results indicated that 19 accessions (25.0%) were heterogeneous for HMW glutenin subunit composition, and contained 2–4 HMW-GS compositions generally. At Glu-1 locus, a total of 14 different glutenin alleles were observed and the number of alleles at Glu-A1, Glu-B1, and Glu-D1 was 2, 7, and 5, respectively. Three novel alleles were identified, consisting of two alleles at Glu-B1 and one allele at Glu-D1 locus. The 14 alleles resulted in 16 different HMW subunit combinations, and the combination (null, 7+8, 2+12) was the major type with the frequency of 69.7%. The genetic diversity indexes for HMW glutenin subunits varied among agro-ecological zones, and were negatively correlated with mean annual precipitation and mean annual temperature. Environmental stress is speculated to be an important factor for the differentiation of diversity in wheat landraces across regions.

Key words: Wheat landrace, HMW-GS, Diversity, Environmental factor

[1]Tian Q Z, Zhou R H, Jia J Z. Genetic diversity trend of common wheat (Triticum aestivum L.) in China revealed with AFLP markers. Genet Resour Crop Evol, 2005, 52: 325–331

[2]Reif J C, Zhang P, Dreisigacker S, Warburton M L, van Ginkel M, Hoisington D, Bohn M, Melchinger A E. Wheat genetic diversity trends during domestication and breeding. Theor Appl Genet, 2005, 110: 859–864

[3]Fu Y, Peterson G W, Richards K W, Somers D, DePauw R M, Clarke J M. Allelic reduction and genetic shift in the Canadian hard red spring wheat germplasm released from 1845 to 2004. Theor Appl Genet, 2005, 110: 1505–1516

[4]Zhu Y Y, Chen H R, Fan J H, Wang Y Y, Li Y, Chen J B, Fan J X, Yang S S, Hu L P, Leung H, Mew T W, Teng P S, Wang Z H, Mundt C C. Genetic diversity and disease control in rice. Nature, 2000, 406: 718–722

[5]Gregová E, Hermuth J, Kraic J, Dotla?il L. Protein heterogeneity in European wheat landraces and obsolete cultivars. Genet Resour Crop Evol, 1999, 46: 521–528

[6]Cai S, Bai G H, Zhang D. Quantitative trait loci for aluminum resistance in Chinese wheat landrace FSW. Theor Appl Genet, 2008, 117: 49–56

[7]Du X-Y(杜小燕), Hao C-Y(郝晨阳), Zhang X-Y(张学勇), Ma Z-Q(马正强), You G-X(游光霞), Wang L-F(王兰芬), Dong Y-C(董玉琛). Genetic diversity of waxy gene in Chinese wheat landraces. Acta Agron Sin (作物学报), 2007, 33(3): 503–506 (in Chinese with English abstract)

[8]Dong Y-C(董玉琛), Zheng D-S(郑殿升). Wheat Genetic Resources in China (中国小麦遗传资源). Beijing: China Agriculture Press, 2000 (in Chinese)

[9]Harlan J R. Our vanishing genetic resources. Science, 1975, 188: 618–621

[10]Zhuang Q-S(庄巧生). Chinese Wheat Improvement and Pedigree Analysis (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003 (in Chinese)

[11]Hirano R, Kikuchi A, Kawase M, Watanabe K N. Evaluation of genetic diversity of bread wheat landraces from Pakistan by AFLP and implications for a future collection strategy. Genet Resour Crop Evol, 2008, 55: 1007–1015

[12]Nakamura H. Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan. Euphytica, 2001, 120: 227–234

[13]Liu Y, Xiong Z Y, He Y G, Shewry P R, He G Y. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet Resour Crop Evol, 2007, 54: 865–874

[14]Ruiza M, Rodriguez-Quijanob M, Metakovskyb E V, Vazquezb J F, Carrillo J M. Polymorphism, variation and genetic identity of Spanish common wheat germplasm based on gliadin alleles. Field Crop Res, 2002, 79: 185–196

[15]He D, Li H, Xu S, Duan X, Zhou Y, Li L. Reaction to powdery mildew and stripe rust in related species and landraces of wheat. Genet Resour Crop Evol, 2007, 54: 213–219

[16]Bajracharya J, Rana R B, Gauchan D, Sthapit B R, Jarvis D I, Witcombe J R. Rice landrace diversity in Nepal. Socio-economic and ecological factors determining rice landrace diversity in three agro-ecozones of Nepal based on farm surveys. Genet Resour Crop Evol, 2010, 57: 1013–1022

[17]Jaradat A A. Grain protein variability among populations of wild barley (Hordeum spontaneum C. Koch.) from Jordan. Theor Appl Genet, 1991, 83: 164–168

[18]Li Y C, Fahima T, Korol A B, Peng J H, Röder M S, Kirzhner V, Beiles A, Nevo E. Microsatellite diversity correlated with ecological-edaphic and genetic factors in three microsites of wild emmer wheat in north Israel. Mol Biol Evol, 2000, 17: 851–862

[19]Lázaro A, Ruiz M, Rosa L, Martín I. Relationships between agro/morphological characters and climatic parameters in Spanish landraces of lentil (Lens culinaris Medik.). Genet Resour Crop Evol, 2001, 48: 239–249

[20]Zhang L-L(张玲丽), Li X-Q(李秀全), Yang X-M(杨欣明), Li H-J(李洪杰), Wang H(王辉), Li L-H(李立会). High-molecular-weight glutenin subunit composition of Chinese wheat germplasm. Sci Agric Sin (中国农业科学), 2006, 39(12): 2406–2414 (in Chinese with English abstract)

[21]Payne P I, Lawrence G J. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutein in hexaploid wheat. Cereal Res Commun, 1983, 11: 29–35

[22]Nei M. Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA, 1973, 70: 3321–3323

[23]Peña R J, Zarco-Hernandez J, Mujeeb-Kazi A. Glutenin subunit compositions and breading-making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum × Triticum tauschii (Coss.) Schmal crosses. J Cereal Sci, 1995, 21: 15–23

[24]Tang Y-L(汤永禄), Yang W-Y(杨武云), Tian J-C(田纪春), Li J(李俊), Chen F(陈放). Effect of HMW-GS 6+8 and 1.5+10 on wheat quality traits in synthetic hexaploid wheat. Agric Sci China (中国农业科学), 2008, 41(11): 3465–3476 (in Chinese with English abstract)

[25]Ruiz M, Metakovsky E V, Rodriguez-Quijano M, Vazquez J F, Carrillo J M. Assessment of storage protein variation in relation to some morphological characters in a sample of Spanish landraces of common wheat (Triticum aestivum L. ssp. aestivum). Genet Resour Crop Evol, 2002, 49: 371–382

[26]Wei Y-M(魏育明), Zheng Y-L(郑有良), Liu D-C(刘登才), Zhou Y-H(周永红), Lan X-J(兰秀锦). Genetic diversity of Gli-1, Gli-2 and Glu-1 alleles in Sichuan wheat landraces. Acta Bot Sin (植物学报), 2000, 42(5): 496–501 (in Chinese with English abstract)

[27]Zhang X-Y(张学勇), Pang B-S(庞斌双), You G-X(游光霞), Wang L-F(王兰芬), Jia J-Z(贾继增), Dong Y-C(董玉琛). Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Agric Sci China (中国农业科学), 2002, 35(11): 1302–1310 (in Chinese with English abstract)

[28]Amirouche R, Misset M T. Hordein polymorphism in diploid and tetraploid Mediterranean populations of the Hordeum murinum L. complex. Plant Syst Evol, 2003, 242: 83–99

[29]Nevo E, Payne P I. Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. Theor Appl Genet, 1987, 74: 827–836

[30]Allard R W. Genomic basis of the evolution of adaptedness in plants. Euphytica, 1996, 92: 1–11

[31]Sertse D, Gailing O, Eliades N, Finkeldey R. Anthropogenic and natural causes in?uencing population genetic structure of Juniperus procera Hochst. ex Endl. in the Ethiopian highlands. Genet Resour Crop Evol, 2011, 58: 849–859

[32]Rana R B, Garforth C, Sthapit B, Jarvis D. In?uence of socio-economic and cultural factors in rice varietal diversity management on-farm in Nepal. Agric Human Values, 2007, 24: 461–472
[1] Ma Xiao-Qian, Qin Na, Dai Shu-Tao, Qin Jia-Fan, Li Xiao-Yan, Wang Shu-Ting, Liu Zhong-Ling, Li Jun-Xia. Phenotypic variation analysis of 175 foxtail millet (Setaria italica) germplasm accessions under two environments [J]. Acta Agronomica Sinica, 2026, 52(6): 1757-1773.
[2] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[3] Xu Jian-Xia, Ding Yan-Qing, Cao Ning, Cheng Bin, Gao Xu, Li Wen-Zhen, Wang Ruo-Ruo, Wang Lei, Zhang Li-Yi. Phenotypic diversity analysis and comprehensive evaluation of 397 sorghum germplasm resources in Guizhou, China [J]. Acta Agronomica Sinica, 2026, 52(4): 1073-1087.
[4] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[5] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
[6] Ma Ting-Ting, Guo Xiao-Jiang, Li Hao, Deng Mei, Pu Zhi-En, Li Wei, Zhang Ya-Zhou, Wang Feng-Tao, Cui Feng-Juan, Wei Yu-Ming, Wang Ji-Rui, Jiang Yun-Feng, Chen Guo-Yue. Breeding strategy for synergistic improvement of yield, disease resistance, and stress tolerance in Shumai 753 using the wheat landrace Xiaoganmai [J]. Acta Agronomica Sinica, 2026, 52(1): 56-71.
[7] GAO Yuan, LI Xia, WEI Shao-Bo, TIAN Xiao-Hai, ZHOU Wen-Bin. Research advances on plant midday depression of photosynthesis [J]. Acta Agronomica Sinica, 2025, 51(9): 2253-2265.
[8] MEI Piao, LIU Ding-Ding, YE Yuan-Yuan, ZHANG Chen-Yu, DING Shi-Qi, LI Ya-Qi, WANG Pei-Xin, MEI Ju-Fen, MA Chun-Lei. Genetic diversity analysis of domestic albino tea germplasm resources based on the tea plant liquid phase functional chip [J]. Acta Agronomica Sinica, 2025, 51(9): 2358-2370.
[9] WANG Tian-Yi, YANG Xiu-Juan, ZHAO Jia-Jia, HAO Yu-Qiong, ZHENG Xing-Wei, WU Bang-Bang, LI Xiao-Hua, HAO Shui-Yuan, ZHENG Jun. Gliadin diversity and its effects on flour quality in wheat from Shanxi province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1784-1800.
[10] WANG Mu, ZHUO Ga, ZHA Sang, XIRUO Qu-Zong, DAWA Dondup, GUO Gang-Gang, ZHANG Jing, ZHUO Ga, LHUNDRUP Namgyal. Genetic diversity analysis and comprehensive evaluation of Qingke germplasm based on six phenotypic traits [J]. Acta Agronomica Sinica, 2025, 51(6): 1526-1537.
[11] XU Xiao-Wei, FENG Jing, WANG Feng-Tao, TONG Zhao-Yang, ZHANG Jian-Zhou, LI Chun-Ying, LIN Rui-Ming. QTL mapping of adult plant resistance to stripe rust in the Chinese wheat landrace Canlaomai [J]. Acta Agronomica Sinica, 2025, 51(11): 2933-2943.
[12] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
[13] CHEN Jia-Wei, LIN Yan, ZHANG Ming-Xing, ZHOU Shi-Jing, RAO Li-Qun, ZHOU Chi, LI Xin. Effects of Bacillus velezensis YCH92 on the rhizosphere microbial community and yield of cotton [J]. Acta Agronomica Sinica, 2025, 51(10): 2821-2835.
[14] TIAN Han-Zhao, FENG Long-Ting, YING Kai, MENG Tian-Qi, WU Jun, LIU Yu-Xiu. Composition and evaluation of wheat glutenin subunits in exotic wheat germplasm [J]. Acta Agronomica Sinica, 2025, 51(10): 2663-2680.
[15] JIANG Xiao, ZHAO Jian-Xin, BI Jing-Nan, XU Jing, YIN Xiang-Zhen, ZHAO Xu-Hong, PAN Li-Juan, CHEN Na, Ma Jun-Qing, HAN Peng, YANG Zhen, CHI Xiao-Yuan. Effects of major geographic and climatic factors on agronomic traits and quality of peanut in the main production areas of northern China [J]. Acta Agronomica Sinica, 2025, 51(10): 2805-2820.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd