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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1205-1211.doi: 10.3724/SP.J.1006.2012.01205

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity of High-Molecular-Weight Glutenin Subunit Composition in Chinese Wheat Landraces

XU Xin1,2,**,LI Xiao-Jun1,3,**,ZHANG Ling-Li1,4,LI Xiu-Quan1,YANG Xin-Ming1,LI Li-Hui1,*   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081, China; 2Department of Life Sciences and Technology, Xinxiang University, Xinxiang 453003, China; 3School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China; 4College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
  • Received:2011-11-24 Revised:2012-02-23 Online:2012-07-12 Published:2012-05-11
  • Contact: 李立会, E-mail: lilihui@caas.net.cn, Tel: 010-62186670 ** 同等贡献(Contributed equally to this work)

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Abstract: The high-molecular-weight glutenin subunit (HMW-GS) composition of 76 representative accessions of wheat landraces, collected from nine agro-ecological zones in China, were examined by sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis (SDS-PAGE). The correlation between diversity indexes at Glu-1 locus and altitude, mean annual precipitation, or mean annual temperature was also analyzed. The results indicated that 19 accessions (25.0%) were heterogeneous for HMW glutenin subunit composition, and contained 2–4 HMW-GS compositions generally. At Glu-1 locus, a total of 14 different glutenin alleles were observed and the number of alleles at Glu-A1, Glu-B1, and Glu-D1 was 2, 7, and 5, respectively. Three novel alleles were identified, consisting of two alleles at Glu-B1 and one allele at Glu-D1 locus. The 14 alleles resulted in 16 different HMW subunit combinations, and the combination (null, 7+8, 2+12) was the major type with the frequency of 69.7%. The genetic diversity indexes for HMW glutenin subunits varied among agro-ecological zones, and were negatively correlated with mean annual precipitation and mean annual temperature. Environmental stress is speculated to be an important factor for the differentiation of diversity in wheat landraces across regions.

Key words: Wheat landrace, HMW-GS, Diversity, Environmental factor

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