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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (02): 216-221.doi: 10.3724/SP.J.1006.2013.00216

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Fine Mapping of Resistance Genes to SMV Strains SC6 and SC17 in Soybean

YANG Xiao-Feng1,2,YANG Yong-Qing1,ZHENG Gui-Jie1,ZHI Hai-Jian1,*,LI Xiao-Hong2   

  1. 1 National Key Laboratory for Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture / National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China; 2 Hunan Crop Research Institute, Changsha 410125, China
  • Received:2012-06-27 Revised:2012-10-05 Online:2013-02-12 Published:2012-12-11
  • Contact: 智海剑, E-mail: zhj@njau.edu.cn

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Abstract:

SMV strains SC6 and SC17 were prevalen in the North China and the Huang-Huai and Yangtze Valleys soybean production regions. Two soybean cultivars Q0926 and Zhongdou 35, which were resistant to SC6 and SC17, were respectively crossed with Nannong 1138-2 and Nannongcaidou 5, which were susceptible to SC6 and SC17, to determine inheritance of resistance to SC6 or SC17. Q0926 was also crossed with Zhongdou 35 to study allelic relationships of the resistance genes from the two soybean cultivars. On the basis of the results, the resistance genes to SC6 and SC17 were fine mapped. The results showed that the F1 plants presented complete resistance, F2 population were segregated with a ratio of 3R:1S, F2:3 population were segregated with a ratio of 1R:2Seg:1S in resistant (R)×susceptible (S) crosses. These results indicated that the resistances of Q0926 and Zhongdou 35 to SC6 and SC17 were controlled by a single dominant gene respectively. The F1 plants displayed complete resistance, and F2 populations of the crosses Q0926×Zhongdou 35 were not segregated, indicating that the resistance genes to SC6 and SC17 were alleles or very closely linked. Genetic maps showed that 25 SSR markers were linked to theresistance gene to SC6 (designated RSC6) and the genetic distance and order of the two closest SSR markers to RSC6 were BARCSOYSSR_02_0622 (0.264 cM)-RSC17- BARCSOYSSR_02_0627 (0.262 cM). The 38 SSR markers were linked tothe resistance gene to SC17 (designated RSC17) and the genetic distance and order of the two closest SSR markers to RSC6 were BARCSOYSSR_02_0622 (0.264 cM)-RSC17 -BARCSOYSSR_02_0627 (0.262 cM),their corresponding physical intervals were 52 kb and 60 kb. The studies on inheritance of resistance to SMV provide a theoretical guidance to programs of resistance breeding to SMV. Fine map of resistance genes laid the foundation for molecular marker-assisted selection and map-based cloning of resistance genes.

Key words: Soybean, Soybean mosaic virus, Inheritance, Allelism, SSR marker

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