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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (02): 216-221.doi: 10.3724/SP.J.1006.2013.00216

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fine Mapping of Resistance Genes to SMV Strains SC6 and SC17 in Soybean

YANG Xiao-Feng1,2,YANG Yong-Qing1,ZHENG Gui-Jie1,ZHI Hai-Jian1,*,LI Xiao-Hong2   

  1. 1 National Key Laboratory for Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture / National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China; 2 Hunan Crop Research Institute, Changsha 410125, China
  • Received:2012-06-27 Revised:2012-10-05 Online:2013-02-12 Published:2012-12-11
  • Contact: 智海剑, E-mail: zhj@njau.edu.cn

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Abstract:

SMV strains SC6 and SC17 were prevalen in the North China and the Huang-Huai and Yangtze Valleys soybean production regions. Two soybean cultivars Q0926 and Zhongdou 35, which were resistant to SC6 and SC17, were respectively crossed with Nannong 1138-2 and Nannongcaidou 5, which were susceptible to SC6 and SC17, to determine inheritance of resistance to SC6 or SC17. Q0926 was also crossed with Zhongdou 35 to study allelic relationships of the resistance genes from the two soybean cultivars. On the basis of the results, the resistance genes to SC6 and SC17 were fine mapped. The results showed that the F1 plants presented complete resistance, F2 population were segregated with a ratio of 3R:1S, F2:3 population were segregated with a ratio of 1R:2Seg:1S in resistant (R)×susceptible (S) crosses. These results indicated that the resistances of Q0926 and Zhongdou 35 to SC6 and SC17 were controlled by a single dominant gene respectively. The F1 plants displayed complete resistance, and F2 populations of the crosses Q0926×Zhongdou 35 were not segregated, indicating that the resistance genes to SC6 and SC17 were alleles or very closely linked. Genetic maps showed that 25 SSR markers were linked to theresistance gene to SC6 (designated RSC6) and the genetic distance and order of the two closest SSR markers to RSC6 were BARCSOYSSR_02_0622 (0.264 cM)-RSC17- BARCSOYSSR_02_0627 (0.262 cM). The 38 SSR markers were linked tothe resistance gene to SC17 (designated RSC17) and the genetic distance and order of the two closest SSR markers to RSC6 were BARCSOYSSR_02_0622 (0.264 cM)-RSC17 -BARCSOYSSR_02_0627 (0.262 cM),their corresponding physical intervals were 52 kb and 60 kb. The studies on inheritance of resistance to SMV provide a theoretical guidance to programs of resistance breeding to SMV. Fine map of resistance genes laid the foundation for molecular marker-assisted selection and map-based cloning of resistance genes.

Key words: Soybean, Soybean mosaic virus, Inheritance, Allelism, SSR marker

[1]Yu Y G, Saghai-Maroof M A, Buss G R, Maughan P J, Tolin S A. RFLP and microsatellite mapping of a gene for soybean mosaic virus resistance. Phytopathology, 1994, 84: 60–64



[2]Hayes A J, Ma G R, Buss G R, Saghai-Maroof M A. Molecular marker mapping of Rsv4, a gene conferring resistance to all known strains of soybean mosaic virus. Crop Sci, 2000, 40: 1434–1437



[3]Jeong S C, Kristipati S, Hayes A J, Maughan P J, Noffsonger S L, Gunduz I, Buss G R, Saghai-Maroof M A. Genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene, Rsv3. Crop Sci, 2002, 42: 265–270



[4]Zhang Z-Y(张志永), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Molecular markers linked to Rsa resistant to soybean mosaic virus. Chin Sci Bull (科学通报), 1994, 44(2): 154–158 (in Chinese with English abstract)



[5]Dong-Fang Y(东方阳). Inheritance of resistance to SMV and research of RAPD marker in soybean. PhD Dissertation of Nanjing Agricultural University, 1999 (in Chinese with English abstract)



[6]Zheng C-M(郑翠明), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Inheritance of resistance to SMV3 and identification of RAPD marker linked to the resistance gene in soybean. Sci Agric Sin (中国农业科学), 2001, 34(1): 1–4 (in Chinese with English abstract)



[7]Wang Y-J(王永军), Dong-Fang Y(东方阳), Wang X-Q(王修强), Yang Y-L(杨雅麟), Gai J-Y(盖钧镒), Wu X-L(吴晓雷), He C-Y(贺超英), Zhang J-S(张劲松), Chen S-Y(陈受宜). Mapping of five genes resistance to SMV strains in soybean. Acta Agron Sin (作物学报), 2004, 31(1): 87–90 (in Chinese with English abstract)



[8]Fu S X, Zhan Y, Zhi H J, Gai J Y, Yu D Y. Mapping of SMV resistance gene Rsc7 by SSR markers in soybean. Genetica, 2006, 128: 63–69



[9]Li H C, Zhi H J, Gai J Y, Guo D Q, Wang Y W, Li K Bai L, Yang H. Inheritance and gene mapping of resistance to soybean mosaic virus strain SC14 in soybean. J Plant Biol, 2006, 48: 1466–1472



[10]Guo D-Q(郭东全), Wang Y-W(王延伟), Zhi H-J(智海剑), Gai J-Y(盖钧镒), Li H-C(李海朝), Li K(李凯). Inheritance and gene mapping of resistance to SMV strain group SC13 in soybean. Soybean Sci (大豆科学), 2007, 26(1): 21–24 (in Chinese with English abstract)



[11]Bai L(白丽), Li H-C(李海朝), Ma Y(马莹), Wang D-G(王大刚), Liu N(刘宁), Zhi H-J(智海剑). Inheritance and gene mapping of resistance to soybean mosaic virus strain SC11 in soybean. Soybean Sci (大豆科学), 2009, 28(1): 1–6 (in Chinese with English abstract)



[12]Wang D G., Ma Y, Liu N, Yang Z L, Zheng G J, Zhi H J. Fine mapping of resistance to soybean mosaic virus strain SC4 in soybean based on genomic-SSR markers. Plant Breed, 2011, 130: 653–659



[13]Wang D G Ma Y, Yang Y Q, Liu N, Li, Song Y P, Zhi H J. Fine mapping and analyses of RSC8 resistance candidate genes to soybean mosaic virus in soybean. Theor Appl Genet, 2011, 122: 555–565



[14]Ma Y, Li H C. Molecular mapping and marker assisted selection of soybean mosaic virus resistance gene RSC12 in soybean. Legume Genom Genet, 2011, 1: 41–46



[15]Wang X-Q(王修强). Strains Identification of Soybean Mosaic Virus in Huang-Huai and the Middle and Lower Changjiang River Valleys and the Resistant Sources as Well as the Inheritance of Resistance in Soybeans. MS Thesis of Nanjing Agricultural University, 2000 (in Chinese with English abstract)



[16]Wang Y-W(王延伟), Zhi H-J(智海剑), Guo D-Q(郭东全), Gai J-Y(盖钧镒), Chen Q-S(陈庆山), Li K(李凯), Li H-C(李海朝). Classification and distribution of strain groups of soybean mosaic virus in Northern China spring planting soybean region. Soybean Sci (大豆科学), 2005, 24(4): 263–268 (in Chinese with English abstract)



[17]Song Q J, Jia G F, Zhu Y L, Grant D, Nelson R T, Hwang E Y, Hyten D L, Cregan P B. Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci, 2010, 50: 1950–1960



[18]Van Ooijen J W, Voorrips R E. JoinMap (R) 3.0, Software for the Calculation of Genetic Linkage Maps. CPRO-DLO, Wageningen.2001



[19]Chen S-Y(陈珊宇), Zheng G-J(郑桂杰), Yang Z-L(杨中路), Liu R-M(刘若淼), Zhi H-J(智海剑). Evaluation of resistance to SMV of soybean core collection from Southern China. Chin J Oil Crop Sci (中国油料作物学报), 2009, 31(4): 513–516 (in Chinese with English abstract)



[20]Li C-Y(李春燕). Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC10 in Soybean. MS Thesis of Nanjing Agricultural University, 2009 (in Chinese with English abstract)



[21]Hwang T Y, Moon J K, Yu S, Yang K, Mohankumar S, Yu Y H, Lee Y H, Kim H S, Kim H M, Saghai-Maroof M A, Jeong S C. Application of comparative genomics in developing molecular markers tightly linked to the virus resistance gene Rsv4 in soybean. Genome, 2006, 49: 380–388



[22]Saghai-Maroof M A, Tucker D M, Skoneczka J A, Bowman B C, Tripathy S, Tolin S A. Fine mapping and candidate gene discovery of the soybean mosaic virus resistance gene, Rsv4. Plant Genome, 2010, 3: 14–22
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