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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 609-616.doi: 10.3724/SP.J.1006.2013.00609

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fine Mapping of Quantitative Traits Loci for Seed Shape Traits in Soybean

NIU Yuan,XIE Fang-Teng,BU Shu-Hong,XIE Shang-Qian,HAN Shi-Feng,GENG Qing-Chun,LIU Bing,ZHANG Yuan-Ming*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-09-10 Revised:2012-12-11 Online:2013-04-12 Published:2013-01-28
  • Contact: 章元明, E-mail: soyzhang@njau.edu.cn, soyzhang@hotmail.com

Abstract:

Based on the target interval region of SSR markers Satt331–Satt592, we constructed 356 RHL-F2 individuals (population I) and 168 recombinant families (population II) derived from 504 F2:6 families of the direct and reciprocal crosses of Lishuizhongzihuang with Nannong 493-1 to fine map quantitative traits loci (QTLs) for seed length (SL), width (SW) and length-to-width (SLW) ratio in soybean using composite interval mapping (CIM) and lasso approaches. Among 168 recombinant families, only one recombinant individual was selected from a same F2:6 family so that 142 recombinant families were constructed as population III. Response variable for QTL analysis was as original observation, and the value corrected by the associated marker information. As a result, markers associated with SL were O19 and S21/Satt331 from lasso method and S21–S22 and O23–O19 from CIM method; marker associated with SW was O19/O21 from lasso method and O23–O19/O19–O21 from CIM method; and the QTL associated with markers S21–S22 for SLW was derived from QTL for SL, and the QTL with markers O23–O19/O19–O21 for SLW was derived from QTLs for SL and SW. This indicates that QTL associated with markers Satt331–Satt592 in our study was further partitioned into two pleiotropic QTLs, which were associated with markers S21–S22 and markers O23–O19/O19–O21. According to the annotation project database, Glyma10g35240 and Glyma10g34980 might be candidate genes for seed shape traits.

Key words: Soybean, Seed shape trait, Quantitative trait locus, Fine mapping, Candidate gene

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