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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1148-1154.doi: 10.3724/SP.J.1006.2013.01148

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Fine Mapping of Rice Lesion Mimic Mutant c5

CHEN Hong-Lin1,2,**,XIANG Yang-Hai1,**,ZHAO Ji-Ying1,2,YIN De-Dong1,2,LIANG Guo-Hua3,ZHAI Wen-Xue1,JIANG Guang-Huai1,*   

  1. 1 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China; 3 Agricultural College of Yangzhou University, Yangzhou 225009, China
  • Received:2012-12-17 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 江光怀, E-mail: ghjiang@genetics.ac.cn, Tel: 010-64807622 E-mail:hlchen@genetics.ac.cn

Abstract:

The lesion mimic mutant c5 was obtained bytreating the seeds of japonica variety Zhonghua 11 with chemical mutagen EMS (ethyl methane sulfonate). The phenotypes and main agronomic traits of the c5 mutant were investigated. There were many nearly circularbrown spots began to appear on c5 leaves at 3-leaf stage. These leaves were stained further with DAB and trypan blue respectively and indicated that there were excessive H2O2 production and clusters of dead cells in the lesion tissues. Compared with the wild-type, the height of c5 plant decreased from 110.4 cm to 74.6 cm, the number of effective tiller per plant and spikelet per panicle were reduced by 23.7% and 28.5% respectively, thousand-grain-weight and seed setting rate were significantly dropped.In addition, c5 is endowed a broad-spectrum resistance to bacterial blight resistance to ten Philippine races of X. oryzae pv. Oryzae (Xoo). Genetic analysis showed that the lesion mimic mutant c5 was controlled by a single recessive nuclear gene. Minghui86 was crossed with c5 and 6269 individuals of F2 population and 18 pairs of molecular markers were used for gene mapping. And thegene was further mapping to rice chromosome 5 in a 102 kb region between STS markers S41 and S47. Sequence analysis of the 102 kb region revealed that there were 11 candidates for c5 gene. All the 11 candidate genes were different from those lesion mimic genes reported previously, which suggested that the phenotype of c5 may be controlled by a novel gene.

Key words: Rice (Oryza sativa L.), Resistance, Lesion mimic, Genetic analysis, Gene mapping

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