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Acta Agronomica Sinica ›› 2013, Vol. 39 ›› Issue (09): 1602-1611.doi: 10.3724/SP.J.1006.2013.01602

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Expression Analysis of Potassium Channel Gene NKT6 in Nicotiana sylvestris

JIN Yi-Rong1,2,BAI Yan1,2,SONG Yu-Feng1,2,ZHANG Liang1,2,DONG Lian-Hong1,2,LIU Chao-Ke3,FENG Xiang-Guo3,HU Xiao-Ming3,WANG Qian1,*,LIU Hao-Bao1,*   

  1. 1 Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China; 2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 China Tobacco Chuanyu Industrial Co. Ltd., Chengdu 610000, China
  • Received:2012-12-31 Revised:2013-04-22 Online:2013-09-12 Published:2013-07-09
  • Contact: 王倩, E-mail: wangqian2000_zb@163.com, Tel: 0532-88701031; 刘好宝, E-mail: l88702236@163.com, Tel: 0532-88701829

Abstract:

Shaker family potassium channels play a crucial role in absorption and transportation of potassium in plants. A new potassium channel gene, designated as NKT6 (GenBank accession number KC310448), was isolated from N. sylvestris using homologous cloning strategy. The full length sequence of NKT6 cDNA was 2137 bp. The deduced protein sequence contained 618 amid acids and exhibited high homology with Shaker family potassium channels. The genomic sequence of NKT6 contained 11 exons and 10 introns. The phylogenetic tree showed that NKT6 belonged to Group II of Shaker family. Real-time quantitative PCR assay indicated that the expression level of NKT6 in stem was the highest at full-bloom stage of Nicotiana sylvestris. Subcellular localization assay showed NKT6 was localized on plasma membrane and endoplasmic reticulum of tobacco epidermal cells. The expression level of NKT6 declined under drought and ABA treatment. These results demonstrated that NKT6 might play a role in stomatal movement.

Key words: Potassium channel, Nicotiana sylvestris, NKT6, Cloing and expression, Subcellular localization

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