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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 174-180.doi: 10.3724/SP.J.1006.2014. 00174

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity Analysis of Wild Adzuki Bean Germplasm and Its Relatives by Using SSR Markers

LIU Chang-You1,FAN Bao-Jie1,CAO Zhi-Min1,SU Qiu-Zhu1,WANG Yan1,ZHANG Zhi-Xiao1,CHENG Xu-Zhen2,TIAN Jing1,*   

  1. 1 Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Hebei Laboratory of Crop Genetic and Breeding, Shijiazhuang 050031, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-07-01 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655 E-mail:Liuchangyou2006@yahoo.com.cn


Wild Adzuki bean (V. angularis var. nipponensis) germplasm and its relatives are very important materials for adzuki bean breeding. In this paper, genetic diversity of 96 accessions of wild types, relatives and cultivated adzuki beans were analyzed by using 28 pairs of polymorphic SSR makers. The results showed that a total of 255 alleles were detected in all the accessions. The average number of allele per SSR locus was 9.10. The range of genetic diversity index (polymorphic information content, PIC value) was from 0.374 to 0.865, with an average of 0.722. The results revealed that there were abundant genetic variations between wild adzuki bean and its relatives Vigna minima. Both cluster and principal coordinate analysis (PCO analysis) suggested that there was a high genetic differentiation between wild adzuki beans and its relatives, and they could be distinguished from each other clearly according to their origins, suggesting that the locations of wild adzuki bean accessions affect their genetic variations. When analyzed the relationship between adzuki bean varieties and wild adzuki bean accessions, we found that their genetic distance was more closer to Japanese wild adzuki bean accessions than to domestic accessions, indicating that more Japanese adzuki bean accessions were used in Chinese adzuki bean breeding, and showing that our works for domestic wild adzuki bean resources collection and use is behind Japan. The present study provides some information in collection and conservation of domestic wild adzuki bean accessions, and the result is useful for evaluating and using these resources to find useful genes.

Key words: Wild adzuki bean, SSR, Relatives of adzuki bean, Genetic diversity

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