Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 174-180.doi: 10.3724/SP.J.1006.2014.00174

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity Analysis of Wild Adzuki Bean Germplasm and Its Relatives by Using SSR Markers

LIU Chang-You1,FAN Bao-Jie1,CAO Zhi-Min1,SU Qiu-Zhu1,WANG Yan1,ZHANG Zhi-Xiao1,CHENG Xu-Zhen2,TIAN Jing1,*   

  1. 1 Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences / Hebei Laboratory of Crop Genetic and Breeding, Shijiazhuang 050031, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-07-01 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 田静, E-mail: nkytianjing@163.com, Tel: 0311-87670655 E-mail:Liuchangyou2006@yahoo.com.cn

Abstract:

Wild Adzuki bean (V. angularis var. nipponensis) germplasm and its relatives are very important materials for adzuki bean breeding. In this paper, genetic diversity of 96 accessions of wild types, relatives and cultivated adzuki beans were analyzed by using 28 pairs of polymorphic SSR makers. The results showed that a total of 255 alleles were detected in all the accessions. The average number of allele per SSR locus was 9.10. The range of genetic diversity index (polymorphic information content, PIC value) was from 0.374 to 0.865, with an average of 0.722. The results revealed that there were abundant genetic variations between wild adzuki bean and its relatives Vigna minima. Both cluster and principal coordinate analysis (PCO analysis) suggested that there was a high genetic differentiation between wild adzuki beans and its relatives, and they could be distinguished from each other clearly according to their origins, suggesting that the locations of wild adzuki bean accessions affect their genetic variations. When analyzed the relationship between adzuki bean varieties and wild adzuki bean accessions, we found that their genetic distance was more closer to Japanese wild adzuki bean accessions than to domestic accessions, indicating that more Japanese adzuki bean accessions were used in Chinese adzuki bean breeding, and showing that our works for domestic wild adzuki bean resources collection and use is behind Japan. The present study provides some information in collection and conservation of domestic wild adzuki bean accessions, and the result is useful for evaluating and using these resources to find useful genes.

Key words: Wild adzuki bean, SSR, Relatives of adzuki bean, Genetic diversity

[1]郑卓杰. 中国食用豆类学. 北京: 中国农业出版社, 1997. pp 3–6



Zheng Z J. Food Legumes in China. Beijing: Chinese Agricultural Press, 1997. pp 3–6 (in Chinese)



[2]王述民, 曹永生, R J Redden, 胡家蓬. 我国小豆种质资源形态多样性鉴定与分类研究. 作物学报, 2002, 28: 727–733



Wang S M, Cao Y S, Redden R J, Hu J P, Usher T. The morphological diversity and classification of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] germplasm resources in China. Acta Agron Sin, 2002, 28: 727–733 (in Chinese with English abstract)



[3]田静, 范保杰. 河北省小豆品种资源主要农艺性状的遗传变异分析. 河北农业大学学报, 2002, 25(4): 17–20



Tian J, Fan B J. Genetic variability of agronomic traits of adzuki bean in Hebei province. J agric univ Hebei, 2002, 25(4): 17–20 (in Chinese with English abstract)



[4]刘长友, 田静, 范保杰. 河北省小豆种质资源遗传多样性分析. 植物遗传资源学报, 2009, 10: 73–76



Liu C Y, Tian J, Fan B J. Genetic diversity analysis of adzuki bean germplasm in Hebei Province. J Plant Genet Resour, 2009, 10: 73–76 (in Chinese with English abstract)



[5]范树国, 张再君, 刘林, 刘鸿先, 梁承邺. 中国野生稻遗传资源的保护及其在育种中的利用, 生物多样性, 2000, 8: 198–207



Fan S G, Zhang Z J, Liu L, Liu H X, Liang C Y. Conservation of wild rice genetic resources in China and their utilization in breeding. Biodiversity, 2000, 8: 198–207 (in Chinese with English abstract)



[6]章琦, 赵炳宇, 赵开军, 王春连, 杨文才, 林世成, 阙更生, 周永力, 李道远, 陈成斌, 朱立煌. 普通野生稻的抗水稻白叶枯病(Xanthomonas oryzae pv. oryzae)新基因Xa-23(t)的鉴定和分子标记定位. 作物学报, 2000, 26: 536–542



Zhang Q, Zhao B Y, Zhao K J, Wang C L, Yang W C, Lin S C, Que G S, Zhou Y L, Li D Y, Chen C B, Zhu L H. Identifying and mapping a new gene Xa-23(t) for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Acta Agron Sin, 2000, 26: 536–542 (in Chinese with English abstract)



[7]王希, 李勇, 朱延明, 柏锡, 才华, 纪巍. 野生大豆胁迫应答膜联蛋白基因的克隆及胁迫耐性分析. 作物学报, 2010, 36: 1666–1673



Wang X, Li Y, Zhu Y M, Bai X, Cai H, Ji W. Cloning and tolerance analysis of gsANN gene related to response on stress in Glycine soja. Acta Agron Sin, 2010, 36: 1666–1673 (in Chinese with English abstract)



[8]Somta P, Kaga A, Tomooka N, Isemura T, Vaughan D A, Srinives P. Mapping of quantitative trait loci for a new source of resistance to bruchids in the wild species Vigna nepalensis Tateishi & Maxted (Vigna subgenus Ceratotropis). Theor Appl Genet, 2008, 117: 621–628



[9]Siriwardhane D, Egawa Y, Tomooka N. Cross compatibility of cultivated adzuki bean (Vigna angularis) and rice bean (V. umbellata) with their wild relatives. Plant Breed, 1991, 107: 320–325



[10]金文林, 文自翔, 濮绍京, 赵波. 应用RAPD分析小豆种质资源遗传多样性及遗传演化趋势. 中国农业科学, 2005, 38: 241–249



Jin W L, Wen Z X, Pu S J, Zhao B. Genetic diversity and evolution of adzuki bean germplasm resources based on RAPD markers. Sci Agric Sin, 2005, 38: 241–249 (in Chinese with English abstract)



[11]陶宛鑫, 濮绍京, 金文林, 赵波, 万平. 野生小豆种质资源植株形态性状多样性分析. 植物遗传资源学报, 2007, 8: 174–178



TaoW X, Pu S J, Jin W L, Zhao B, Wan P. The morphological diversity analysis of wild adzuki bean germplasm resources. J Plant Genet Resour, 2007, 8: 174–178 (in Chinese with English abstract)



[12]赵波, 叶剑, 金文林, 曾潮武, 吴宝美, 濮绍京, 潘金豹, 万平. 不同类型小豆种质SSR标记遗传多样性及性状关联分析.中国农业科学, 2011, 44: 673–682



Zhao B, Ye J,Jin W L, Zeng C W, Wu B M, Pu S J, Pan J B, Wan P. Analysis on genetic diversity and trait association of different types of adzuki bean (Vigna angularis) by SSR markers. Sci Agric Sin, 2011, 44: 673–682 (in Chinese with English abstract)



[13]宗绪晓, Duancan Vaughan, Norihiko Tomooka, Akito Kaga, 王新望, 关建平, 王述民. AFLP初析小豆栽培和野生变种(Vigna angularis var.angularis and var.nipponensis)间演化与地理分布关系. 中国农业科学, 2003, 36: 367–374



Zong X X,Vaughan D, Tomooka N, Kaga A, Wang X W, Guan J P, Wang S M. Preliminary study on geographical distribution and evolutionary relationships between cultivated and wild adzuki bean (Vigna angularis var.angularis and var. nipponensis) by AFLP analysis. Sci Agric Sin, 2003, 36: 367–374 (in Chinese with English abstract)



[14]Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for adzuki bean [Vigna angularis (Willd.) Ohwi and Ohashi]. Theor Appl Genet, 2005, 111: 1278–1287



[15]Nei M, Tajima F, Tateno Y. Accuracy of estimated phylogenetic trees from molecular data: II. Gene frequency data. J Mol Evol, 1983, 19: 153–170



[16]文自翔, 金文林, 濮绍京, 赵波. 遗传标记在小豆遗传育种研究中的应用. 北京农学院学报, 2003, 18(1): 62–68



Wen Z X, Jin W L, Pu S J, Zhao B. The application of genetic marker in genetics and breeding of adzuki beans. J Beijing Agric Coll, 2003, 18(1): 62–68 (in Chinese with English abstract)



[17]王述民, 张赤红. 利用AFLP标记鉴定小豆种质遗传多样性, 植物遗传资源学报, 2002, 3(3): 1–5



Wang S M, Zhang C H. Genetic diversity of adzuki bean germplasm revealed by AFLP marker. J Plant Genet Resour, 2002, 3(3): 1–5 (in Chinese with English abstract)



[18]宗绪晓, D Vaughan, A Kaga, N Tomooka, 王新望, 关建平, 王述民. AFLP分析小豆种(Vigna angularis)内遗传多样性. 作物学报, 2003, 29: 562–568



Zong X X, Vaughan D, Kaga A, Tomooka N, Wang X W, Guan J P, Wang S M. Genetic diversity in Vigna angularis revealed by AFLP analysis. Acta Agron Sin, 2003, 29: 562–568 (in Chinese with English abstract)



[19]粟生群, 荣廷昭, 余跃辉, 刘坚. 利用AFLP标记鉴定小豆栽培型种质遗传多样性. 四川农业大学学报, 2005, 23(2): 156–162



Su S Q, Rong T Z, Yu Y H, Liu J. Genetic diversity in cultivated adzuki bean germplasm resources revealed by AFLP markers. J Sichuan Agric Univ, 2005, 23(2): 156–162 (in Chinese with English abstract)



[20]Wang X W, Kaga A, Tomooka N, Vaughan D A. The development of SSR markers by a new method in plants and their application to gene flow studies in adzuki bean [Vigna angularis (Willd.) Ohwi and Ohashi]. Theor Appl Genet, 2004, 109: 352–360



[21]徐宁, 程须珍, 王丽侠, 王素华, 刘长友, 孙蕾, 梅丽. 用于中国小豆种质资源遗传多样性分析SSR分子标记筛选及应用.作物学报, 2009, 35: 219−227



Xu N, Cheng X Z, Wang L X, Wang S H, Liu C Y, Sun L, Mei L. Screening and application of SSR molecular markers for genetic diversity analysis of Chinese adzuki bean germplasm resources. Acta Agron Sin, 2009, 35: 219−227 (in Chinese with English abstract)



[22]王丽侠, 程须珍, 王素华, 梁辉, 赵丹, 徐宁. 应用SSR标记对小豆种质资源的遗传多样性分析. 作物学报, 2009, 35: 1858−1865



Wang L X, Cheng X Z, Wang S H, Liang H, Zhao D, Xu N. Genetic diversity of adzuki bean germplasm resources revealed by SSR markers. Acta Agron Sin, 2009, 35: 1858−1865 (in Chinese with English abstract)



[23]王丽侠, 程须珍, 王素华, 徐宁, 梁辉, 赵丹. 利用SSR标记分析小豆种质资源的遗传多样性. 中国农业科学, 2009, 42: 2661–2666



Wang L X, Cheng X Z, Wang S H, Xu N, Liang H, Zhao D. Genetic diversity among adzuki bean germplasm revealed by SSR markers. Sci Agric Sin, 2009, 42: 2661–2666 (in Chinese with English abstract)



[24]王丽侠, 程须珍, 王素华. 基于SSR标记分析小豆及其近缘植物的遗传关系. 生物多样性, 2011, 19: 17–23



Wang L X, Cheng X Z, Wang S H. Genetic diversity in adzuki bean and its relatives based on SSR markers. Biodivers Sci, 2011, 19: 17–23 (in Chinese with English abstract)



[25]Xu H X, Ting J, Kaga A, Tomooka N, Isemura T, Vaughan D A. Genetic diversity of cultivated and wild azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] as assessed by SSR markers. Genome, 2008, 51: 728–738



[26]Tomooka N, Kashiwaba K, Vaughan D A, Ishimoto M, Egawa Y. The effectiveness of evaluating wild species: searching for sources of resistance to bruchid beetles in the genus Vigna subgenus Ceratotropis. Euphytica, 2000, 115: 27–41

[1] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[2] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[3] Xu Jian-Xia, Ding Yan-Qing, Cao Ning, Cheng Bin, Gao Xu, Li Wen-Zhen, Wang Ruo-Ruo, Wang Lei, Zhang Li-Yi. Phenotypic diversity analysis and comprehensive evaluation of 397 sorghum germplasm resources in Guizhou, China [J]. Acta Agronomica Sinica, 2026, 52(4): 1073-1087.
[4] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[5] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
[6] MEI Piao, LIU Ding-Ding, YE Yuan-Yuan, ZHANG Chen-Yu, DING Shi-Qi, LI Ya-Qi, WANG Pei-Xin, MEI Ju-Fen, MA Chun-Lei. Genetic diversity analysis of domestic albino tea germplasm resources based on the tea plant liquid phase functional chip [J]. Acta Agronomica Sinica, 2025, 51(9): 2358-2370.
[7] WANG Tian-Yi, YANG Xiu-Juan, ZHAO Jia-Jia, HAO Yu-Qiong, ZHENG Xing-Wei, WU Bang-Bang, LI Xiao-Hua, HAO Shui-Yuan, ZHENG Jun. Gliadin diversity and its effects on flour quality in wheat from Shanxi province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1784-1800.
[8] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
[9] ZHENG Dong, ZHOU Xian-Li, TENG Chang-Cai, HOU Wan-Wei, ZHANG Hong-Yan, LIU Yu-Jiao. Genetic relationship analysis and fingerprints construction of faba bean varieties in Qinghai province based on SSR markers [J]. Acta Agronomica Sinica, 2025, 51(1): 79-90.
[10] KUANG Bo-Wen, WEI Ni, LIU Jin-Dian, CHEN Mei-Yan, MAO Xing-Jie, DUAN Wei-Xing, YANG Xi-Ping. Development of SSR markers and database based on genomes of sugarcane and its relatives [J]. Acta Agronomica Sinica, 2025, 51(1): 103-116.
[11] ZHANG Hong-Yan, MIN Yu-Xia, TENG Chang-Cai, PENG Xiao-Xing, CHEN Zhi-Kai, ZHOU Xian-Li, LOU Shu-Bao, LIU Yu-Jiao. Genetic diversity analysis of Chinese faba bean (Vicia faba L.) germplasm resources using 130K liquid phase chips [J]. Acta Agronomica Sinica, 2024, 50(8): 1989-2000.
[12] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, CAO Yue, CHEN Xi-Ming, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Core germplasm identification of proso millet in spring sowing area of Northeast China using fluorescent microsatellite markers [J]. Acta Agronomica Sinica, 2024, 50(7): 1728-1739.
[13] LI Chang-Xi, DONG Zhan-Peng, GUAN Yong-Hu, LIU Jin-Wei, LI Hang, MEI Yong-Jun. Genetic contribution and decision coefficient analysis of agronomic characters and lint yield traits of upland cotton in southern Xinjiang [J]. Acta Agronomica Sinica, 2024, 50(6): 1486-1502.
[14] MA Yan-Ming, LOU Hong-Yao, WANG Wei, SUN Na, YAN Guo-Rong, ZHANG Sheng-Jun, LIU Jie, NI Zhong-Fu, XU Lin. Genetic difference and genome association analysis of grain quality traits in Xinjiang winter wheat [J]. Acta Agronomica Sinica, 2024, 50(6): 1394-1405.
[15] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, GUO Juan, Dipak K SANTRA, WANG Rui-Yun, CHEN Xi-Ming. Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China [J]. Acta Agronomica Sinica, 2024, 50(5): 1181-1192.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!