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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 166-173.doi: 10.3724/SP.J.1006.2014.00166

• RESEARCH NOTES • Previous Articles     Next Articles

Ultrastructure of Central Cell before and after Fertilization in Sugar Beet (Beta vulgaris)

LI Wei1,2,SHEN Jia-Heng2,*,GUO De-Dong3   

  1. 1 Alkali Soil Natural Environmental Science Center, Northeast Forestry University / Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China; 2 College of Life Sciences and Technology, Harbin Normal University / Key Laboratory of Plant Biology, College of Heilongjiang Province, Harbin 150025, China; 3 College of Life Sciences, Heilongjiang University, Harbin 150070, China
  • Received:2013-07-22 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 申家恒, E-mail: hsdshenjiaheng@163.com E-mail:hsdliwei@163.com

Abstract:

The ultrastructural changes of central cell before and after fertilization were studied with TEM to provide more information for reproductive biology of sugar beet and relative research. The results indicated that the changes of central cell before and after fertilization mainly occurred in nucleus and cytoplasm around. Fusion of polar nuclei took place early at bud stage. The nucleolus of secondary nucleus was bipolar with notable fibrous and particle core. Sometimes, there was extra small nucleolus and nucleolus vacuole. Cytoplasm surrounding secondary nucleus was rich in organelles, including mitochondria, plastids with or without starch grain, Golgi body, ribosomes and rough endoplasmic reticulum (rER). After fertilization, a striking feature of cytoplasm surrounding nucleus was the well developed amoeboid plastids, which were numerous and various, filling with starch gains. Soon after that, primary endosperm nucleus divided into two free nuclei. During division, nucleolus disappeared and nuclear membrane disintegrated into small vesicles. Also, there was an obvious decrease in rER and an increase in smooth endoplasmic reticulum (sER). Overall, central cell was active before and after fertilization.

Key words: Sugar beet (Beta vulgaris), Central cell, Fertilization, Ultrastructure

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