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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (05): 823-829.doi: 10.3724/SP.J.1006.2014.00823

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Rice Variety Based on ID Used SSR Fingerprint and Commodity Information

LU Xu-Zhong1,NI Jin-Long1,LI Li1,WANG Xiu-Feng1,MA Hui1,ZHANG Xiao-Juan1,2,YANG Jian-Bo1,*   

  1. 1 Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 2.Institute of Cotton Research, Anhui Academy of Agricultural Sciences, Anqing 246003, China
  • Received:2013-09-23 Revised:2013-12-12 Online:2014-05-12 Published:2014-02-14
  • Contact: 杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212
  • About author:杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212

Abstract:

For increasing convenience of the rice seed quality tracking and management, we selected a set of 12 rice SSR markers (one marker for each chromosome) based on previous research. The fluorescent marker analysis was performed on 127 released and popularized authorized (or main plant) rice varieties in Anhui Province. Then, the rice variety ID was constructed by the SSR fingerprint with corresponded commodity information. The rice variety ID included three components: part 1 was recognized as commodity code, which reflected crop species, cultivar, breeding (or approval) region and year; part 2 was recognized as fingerprint code, which contained the variety DNA fingerprint information; part 3 was recognized as complement code (specific gene identification code), reflecting specific genetic information of cultivars. We also described the rice variety ID in the form of bar code or QR code, which might be favorable for rice seed identification and traceability management.

Key words: Rice, Fluorescent SSR Markers, Capillary Electrophoresis, Commodity Information, Variety ID

[1]Ni X Y, Lei W X, Zhao J Y. Establishment of molecular identity ID Brassica napus varieties using SSR markers. Cruciferous oil crops and sustainable development. In: The Paper Abstract of 12th International Conference on Rapeseed. Monmouth Junction: Scientific American Publishing Company, 2007. p 166



[2]高运来, 朱荣胜, 刘春燕, 李文福, 蒋洪蔚, 李灿东, 姚丙晨, 胡国华, 陈庆山. 黑龙江部分大豆品种分子ID的构建. 作物学报, 2009, 35: 211–218



Gao Y L, Hu R S, Liu C Y, Li W F, Jiang H W, Li C D, Yao B C, Hu G H, Chen Q S. Establishment of molecular ID in soybean varieties in Heilongjiang, China. Acta Agron Sin, 2009, 35: 211–218 (in Chinese with English abstract).



[3]陈昌文, 曹珂, 王力荣, 朱更瑞, 方伟超. 中国桃主要品种资源及其野生近缘种的分子身份证构建. 中国农业科学, 2011, 44: 2081–2093



Chen C W, Cao K, Wang L R, Zhu G R, Fang W C. Molecular ID establishment of main China peach varieties and peach related species. Chin Agric Sci, 2011, 44: 2081–2093 (in Chinese with English abstract)



[4]王黎明, 焦少杰, 姜艳喜, 严洪冬, 苏德峰, 孙广全. 142份甜高粱品种的分子身份证构建. 作物学报, 2011, 37: 1975–1983



Wang L M, Jiao S J, Jiang Y X, Yan H D, Su D F, Sun G Q. Establishment of molecular identity in 142 sweet sorghum varieties. Acta Agron Sin, 2011, 37: 1975–1983 (in Chinese with English abstract)



[5]丁俊杰, 姜翠兰, 顾鑫, 杨晓贺, 赵海红, 申宏波, 仕相林, 刘春燕, 胡国华, 陈庆山. 利用与大豆灰斑病抗性基因连锁的SSR标记构建大豆品种(系)的分子身份证. 作物学报, 2012, 38: 2206–2216



Ding J J, Jiang C L, Gu X, Yang X H, Zhao H H, Shen H B, Shi X L, Liu C Y, Hu G H, Chen Q S. Establishment of molecular ID of soybean varieties (lines) using SSR markers linked to resistance genes against Cercospora sojina. Acta Agron Sin, 2012, 38: 2206–2216 (in Chinese with English abstract)



[6]颜静宛, 田大刚, 许彦, 王锋. 杂交稻主要亲本的SSR分子身份证数据库的构建. 福建农业学报, 2011, 26: 148–152



Yan J W, Tian D G, Xu Y, Wang F. Constructing SSR molecular database for identity system of hybrid rice parents. Fujian J Agric Sci, 2011, 26: 148–152 (in Chinese with English abstract).



[7]杨剑波. 杂交水稻亲本SSR指纹图谱. 合肥: 安徽科学技术出版社, 2012



Yang J B. SSR Fingerprint of Hybrid Rice Parents. Hefei: Anhui Science and Technology Publishing House, 2012



[8]辜大川, 邱东峰, 殷明珠, 焦春海, 杨金松, 张再君, 刘春萍. 水稻优异资源分子身份证的建立. 湖北农业科学, 2012, 51: 5580–5583



Gu D C, Qiu D F, Yin M Z, Jioa C H, Yang J S, Zhang Z J, Liu C P. Establishment of molecular identity card of elite rice resources. Hubei Agric Sci, 2012, 51: 5580–5583 (in Chinese with English abstract)



[9]马琳, 刘海珍, 陆徐忠, 倪金龙, 张小娟, 杨剑波. 130份甘蓝型油菜种质分子身份证的构建. 中国油料作物学报, 2013, 35: 231–239



Ma L, Liu H Z, Lu X Z, Ni J L, Zhang X J, Yang J B. Establishment molecular identity of 130 Brassica napus varieties. Chin J Oil Crop Sci, 2013, 35: 231–239 (in Chinese with English abstract)



[10]杨剑波, 姚剑, 陆徐忠, 倪金龙, 张萍, 陈雪娇, 郑海松, 李云飞, 从夕汉, 刘海珍, 张小娟, 马琳, 朱金国. 两系水稻品种真实性与纯度鉴定DNA分析法(SN/T 3402-2012). 国家质量监督检验检疫总局, 2012



Yang J B, Yao J, Lu X Z, Ni J L, Zhang P, Chen X J, Zheng H S, Li Y F, Cong X H, Liu H Z, Zhang X J, Ma L, Zhu J G. Identification genuineness and purity of two line rice varieties using DNA analysis method (SN/T 3402 2012). General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, 2012



[11]陆徐忠, 从夕汉, 刘海珍, 倪金龙, 马琳, 李莉, 倪大虎, 杨剑波. 杂交水稻亲本分子身份证及杂SSR指纹数据库的建立. 核农学报, 2012, 26: 853–861



Lu X Z, Cong X H, Liu H Z, Ni J L, Ma L, Li L, Ni D H, Yang J B. Establishment of DNA fingerprinting database for parental lines of hybrid rice. Acta Nucl Agric Sin, 2012, 26: 853–861 (in Chinese with English abstract)



[12]易红梅, 王凤格, 赵久然, 王璐, 郭景伦, 原亚萍. 玉米品种SSR标记毛细管电泳荧光检测法与变性PAGE银染检测法的比较研究. 华北农学报, 2006, 21(5): 64–67



Yi H M, Wang F G, Zhao J R, Wang L, Guo J L, Yuan Y P. Comparison of two maize SSR detection methods: Capillary electrophoresis with fluorescence detection method and denaturing PAGE silver staining detection method. Acta Agric Boreal Sin, 2006, 21(5): 64–67 (in Chinese with English abstract)



[13]程本义, 夏俊辉, 龚俊义, 杨仕华. SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用. 中国水稻科学, 2011, 25: 672–676



Cheng B Y, Xia J H, Gong J Y, Yang S H. Application of capillary electrophoresis detection with fluorescent SSR markers in rice DNA fingerprint identification. Chin J Rice Sci, 2011, 25: 672–676 (in Chinese with English abstract)



[14]郭卫华, 张成海, 李素彩, 黄泽霞. 128码(GB/T 18347-2001). 国家质量监督检验检疫总局, 2001



Guo W H, Zhang C H, Li S C, Huang Z X. Code 128 (GB/T 18347-2001). General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, 2001



[15]张成海, 郭卫华, 罗秋科, 黄燕槟, 赵楠. 快速响应矩阵码(GB/T 18284-2000). 国家质量监督检验检疫总局, 2000



Zhang C H, Guo W H, Luo Q K, Huang Y B, Zhao N. QR Code (GB/T 18284-2000). General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, 2000



[16]赵久然, 王凤格, 郭景伦, 陈刚, 廖琴, 孙世贤, 陈如明, 刘龙洲. 中国玉米新品种DNA指纹库建立系列研究: II. 适于玉米自交系和杂交种指纹图谱绘制的SSR核心引物的确定. 玉米科学, 2003, 11(2): 3–5



Zhao J R, Wang F G, Guo J L, Chen G, Liao Q, Sun S X, Cheng R M, Liu L Z. Series of research on establishing DNA fingerprinting pool of Chinese new maize cultivars: II. Confirmation of a set of SSR core primer pairs. J Maize Sci, 2003, 11(2): 3–5 (in Chinese with English abstract)



[17]刘峰, 冯雪梅, 钟文, 刘玉栋, 阴祖军, 韩秀兰, 徐子初, 沈法富. 适合棉花品种鉴定的SSR核心引物的筛选. 分子植物育种, 2009, 7: 1160–1168



Liu F, Feng X M, Zhong W, Liu Y D, Yin Z J, Han X L, Xu Z C, Shen F F. Screening of SSR core primer pairs for identificating cotton cultivar. Mol Plant Breed, 2009, 7: 1160–1168 (in Chinese with English abstract)



[18]郝晨阳, 王兰芬, 贾继增, 董玉琛, 张学勇. SSR荧光标记和银染技术的比较分析. 作物学报, 2005, 31: 144–149



Hao C Y, Wang L F, Jia J Z, Dong Y C, Zhang X Y. Comparison of fluorescence and silver staining detection systems of microsatellite markers. Acta Agron Sin, 2005, 31: 144–149 (in Chinese with English abstract)



[19]刘晓鑫, 谢传晓, 赵琦, 路明, 曲延英, 张滨, 梁业红, 张世煌. 基于SSR荧光标记技术的玉米群体混合样本基因频率分析方法. 中国农业科学, 2008, 41: 3991–3998



Liu X X, Xie C X, Zhao Q, Lu M, Qu Y Y, Zhang B, Liang Y H, Zhang S H. Establishment of fluorescent SSR technique on detecting allelic frequency in maize (Zea mays L.) populations with bulk sampling strategy. Sci Agric Sin, 2008, 41: 3991–3998 (in Chinese with English abstract)



[20]龚亚明, 胡齐赞, 毛伟华, 李亚丹, 张古文, 丁桔. EST SSR荧光标记毛细管电泳检测法在豌豆上的应用及评价. 浙江农业学报, 2009, 21: 540–543



Gong Y M, Hu Q Z, Mao W H, Li Y D, Zhang G W, Ding J. Application and evaluation of fluorescent EST SSR markers detection technique with capillary electrophoresis in pea. Acta Agric Zhejiangensis, 2009, 21: 540–543 (in Chinese with English abstract)



[21]高源, 田路明, 刘凤之, 曹玉芬. 利用SSR荧光标记构建92个梨品种指纹图谱. 园艺学报, 2012, 39: 1437–1446



Gao Y, Tian L M, Liu F Z, Cao Y F. Using the SSR fluorescent labeling to establish SSR fingerprints for 92 cultivars in pyrus. Acta Hortic Sin, 2012, 39: 1437–1446 (in Chinese with English abstract)



[22]余欣荣. 物联网——改变农业、农民、农村的新力量. 合肥: 安徽科学技术出版社, 2012



Yu X R. The Internet of Things—a new power to change agriculture, farmers and rural. Hefei: Anhui Science and Technology Publishing House, 2012

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