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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1585-1594.doi: 10.3724/SP.J.1006.2014.01585

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification, Classification, and Drought Response of F-box Gene Family in Foxtail Millet

HUO Dong-Ying1,2,ZHENG Wei-Jun1,LI Pan-Song1,2,XU Zhao-Shi2,ZHOU Yong-Bin1,2,CHEN Ming2,MA You-Zhi2,MIN Dong-Hong1,2,*,ZHANG Xiao-Hong1,2,*   

  1. 1 State Key Laboratory of Crop Stress Biology for Arid Areas / Northwest Agricultural and Forestry University, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China?
  • Received:2013-12-01 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-09
  • Contact: 张小红, E-mail: zhxh2493@126.com; 闵东红, E-mail: mdh2493@126.com E-mail:huodongyingyr@126.com

Abstract:

F-box proteins play critical roles in plant growth and development and respond to abiotic stresses, involving in cell-cycle regulation, cell apoptosis and signal transduction. Foxtail millet has a character with drought-tolerance. We identified 19 up-regulated F-box genes from 525 F-box families under stress in foxtail millet based on the transcriptome sequencing and divided them into six subgroups, and the genes in the same subgroup had the same intron-exon structure. These genes were unevenly distributed on eight foxtail millet chromosomes, and there were six genes on chromosome 2, that gene number was more than that contained on other chromosomes. The analysis of conserved domain of 19 genes showed that all the protein sequences had a conserved F-box motif, and several other domain also existed in C-terminal including FBD, WD40, FBA, ZnF, and Kelch LRR. Putative cis-acting elements showed that the number of MYB and MYC was the most, which ranged from 9 to 78. Transcriptome sequencing showed that SiF-box18 was more hypersensitive to the drought stress than other F-box members and response to drought, salt, ABA, GA, SA and JA based on the real time PCR. The transient expression in Arabidopsis protoplasts displayed that SiF-box18 protein was localized in nuclear section. This study provided experimental data for further studying the functions of the SiF-box18 gene.

Key words: Foxtail millet, F-box protein, Subcellular localization, Drought response, Induction mechanism

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