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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 651-657.doi: 10.3724/SP.J.1006.2015.00651

• RESEARCH NOTES • Previous Articles     Next Articles

Molecular Identification of Leaf Rust Resistance Gene in Wheat Line 5R625

QIN Jin-Yan1,LI Zai-Feng2,YAN Xiao-Cui1,SU Ji-Hua1,YAO Zhan-Jun1,*,LIU Da-Qun2,*   

  1. 1 College of Agronomy, Agricultural University of Hebei / North China Key Laboratory for Germplasm Resources of Education Ministry, Baoding 071001, China; 2 College of Plant Protection, Agricultural University of Hebei / Biological Control Center for Plant Disease Pests of Hebei Province, Baoding 071001, China
  • Received:2014-09-22 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-03
  • Contact: 姚占军, E-mail: yzhj201@163.com; 刘大群, E-mail: ldq@hebau.cn E-mail:qinjya@163.com

Abstract:

Wheat line 5R625 shows high resistance to all of Puccinia triticinapathotypes at seedling and adult plant stages, but its resistance mechanism is unclear. In this study, the resistance gene(s) were postulated by artificially inoculating 15 P. triticinapathotypes at the seedling stage and comparing infection types with those of 36 wheat lines with known Lr genes. The result showed that 5R625 might carry Lr9, Lr19, Lr24, Lr28, Lr39, Lr47, Lr51,and Lr53. Further genetic analysis to confirm the resistance gene(s) was carried out using the populations of Fl, F2, and F2:3 derived from the cross between 5R625 and Zhengzhou 5389 (susceptible control). A single dominant gene was found responsible for the resistance at seedling and adult plant stages. This gene was mapped on 3DL chromosome with molecular markers using the F2:3 lines. The linkage map contained five closely linked markers, including STS marker 24-16 and SCAR marker OP-J09 that have proved to be cosegregated with Lr24. Therefore, the leaf rust resistance gene in 5R625is most likely Lr24.

Key words: Wheat, Leaf rust resistance gene, Lr24, SSR markers, Molecular mapping

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