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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 690-695.doi: 10.3724/SP.J.1006.2016.00690


Map-based Cloning and Functional Analysis of Low Chlorophyll Fluorescence Gene LCF3 in Arabidopsis thaliana

LIU Ling-Yun,LIU Hao,ZHAO Jing,WANG Yan-Xia,WANG Peng-Tao*   

  1. Henan Key Laboratory of Plant Stress Biology / State Key Laboratory of Cotton Biology / College of Life Sciences, Henan University, Kaifeng 475004, China
  • Received:2015-06-09 Revised:2016-01-11 Online:2016-05-12 Published:2016-01-25
  • Contact: 王棚涛, E-mail: wangpt126@126.com E-mail:lingyunl@henu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31170253) and the Basic and Advanced Technology Research Project in Henan Province (142300413206).


Chlorophyll fluorescence has exclusive role for determining the kinetics of light energy absorption, transmission, dissipation and distribution in leaf photosynthesis. In this study, a mutant was screened by chlorophyll fluorescence equipment from EMS mutagenesis of wild-type Arabidopsis Col-0, which showed lower Fv/Fm than wild-type and was named lcf3-1 (lower chlorophyll fluorescence 3-1). Genetic analysis of lcf3-1 mutant suggested that the mutation was controlled by single recessive gene. LCF3 gene, which encodes PsbW protein, wasisolated by map-based cloning. The T-DNA insertion mutant lcf3-2 showed low chlorophyll fluorescence phenotype as lcf3-1,and this phenotype could be complemented by LCF3 gene of wild-type. These experiments implied that the phenotype of low-chlorophyll-fluorescence resulted from LCF3 gene mutation. Furthermore, our results indicated that LCF3 protein is located in chloroplast and LCF3 gene is expressed in various tissues. PsbW protein is important for the contact and stability between several PSII-LHCII supercomplexes.

Key words: Arabidopsis thaliana, Chlorophyll fluorescence, Photosynthesis, Map-based cloning

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