Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 690-695.doi: 10.3724/SP.J.1006.2016.00690

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Map-based Cloning and Functional Analysis of Low Chlorophyll Fluorescence Gene LCF3 in Arabidopsis thaliana

LIU Ling-Yun,LIU Hao,ZHAO Jing,WANG Yan-Xia,WANG Peng-Tao*   

  1. Henan Key Laboratory of Plant Stress Biology / State Key Laboratory of Cotton Biology / College of Life Sciences, Henan University, Kaifeng 475004, China
  • Received:2015-06-09 Revised:2016-01-11 Online:2016-05-12 Published:2016-01-25
  • Contact: 王棚涛, E-mail: wangpt126@126.com E-mail:lingyunl@henu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31170253) and the Basic and Advanced Technology Research Project in Henan Province (142300413206).

Abstract:

Chlorophyll fluorescence has exclusive role for determining the kinetics of light energy absorption, transmission, dissipation and distribution in leaf photosynthesis. In this study, a mutant was screened by chlorophyll fluorescence equipment from EMS mutagenesis of wild-type Arabidopsis Col-0, which showed lower Fv/Fm than wild-type and was named lcf3-1 (lower chlorophyll fluorescence 3-1). Genetic analysis of lcf3-1 mutant suggested that the mutation was controlled by single recessive gene. LCF3 gene, which encodes PsbW protein, wasisolated by map-based cloning. The T-DNA insertion mutant lcf3-2 showed low chlorophyll fluorescence phenotype as lcf3-1,and this phenotype could be complemented by LCF3 gene of wild-type. These experiments implied that the phenotype of low-chlorophyll-fluorescence resulted from LCF3 gene mutation. Furthermore, our results indicated that LCF3 protein is located in chloroplast and LCF3 gene is expressed in various tissues. PsbW protein is important for the contact and stability between several PSII-LHCII supercomplexes.

Key words: Arabidopsis thaliana, Chlorophyll fluorescence, Photosynthesis, Map-based cloning

[1]Qin X, Suga M, Kuang T, Shen J R. Photosynthesis Structural basis for energy transfer pathways in the plant PSI-LHCI supercomplex. Science, 2015, 348: 989–995



[2]Waters M T, Wang P, Korkaric M. GLK transcription factors coordinate expression of the photosynthetic apparatus in Arabidopsis. Plant Cell, 2009, 21: 1109–1128



[3]Barber J. Photosystem II: an enzyme of global significance. Biochem Soc Trans, 2006, 34: 619–631



[4]Shi L X, Schröder W P. The low molecular mass subunits of the photosynthetic supracomplex, photosystem II. Biochim Biophys Acta. 2004, 1608: 75–96



[5]Irrgang K D, Shi L X, Funk C, Schröder W P. A nuclear-encoded subunit of the photosystem II reaction center. J Biol Chem, 1995, 270: 17588–17593



[6]Lorkovi? Z J, Schröder W P, Pakrasi H B, Irrgang K D, Herrmann R G, Oelmüller R. Oelmuller R. Molecular characterization of PsbW, a nuclearencoded component of the photosystem-II reaction-center complex in spinach. Proc Natl Acad Sci USA, 1995, 92: 8930–8934



[7]Shi L X, Schröder W P. Compositional and topological studies of the PsbW protein in spinach thylakoid membrane. Photosynth Res, 1997, 53: 45–53



[8]Bishop C L, Purton S, Nugent J H. Molecular analysis of the Chlamydomonas nuclear gene encoding PsbW and demonstration that PsbW is a subunit of photosystem II, but not photosystem I. Plant Mol Biol, 2003, 52: 285–289



[9]Shi L X, Lorkovic Z J, Oelmuller R, Schröder W P. The low molecular mass PsbW protein is involved in the stabilization of the dimeric photosystem II complex in Arabidopsis thaliana. J Biol Chem, 2000, 275: 37945–37950



[10]Rokka A, Suorsa M, Saleem A, Battchikova N, Aro E M. Synthesis and assembly of thylakoid protein complexes: multiple assembly steps of photosystem II. Biochem J, 2005, 388: 159–168



[11]Thidholm E, Lindstrom V, Tissier C, Robinson C, Schröder W P, Funk C. Novel approach reveals localisation and assembly pathway of the PsbS and PsbW proteins into the photosystem II dimer. FEBS Lett, 2002, 513: 217–222



[12]Granvogl B, Zoryan M, Ploscher M, Eichacker L A. Localization of 13 one-helix integral membrane proteins in photosystem II subcomplexes. Anal Biochem. 2008, 383: 279–288



[13]Dobáková M, Sobotka R, Tichý M, Komenda J. Psb28 protein is involved in the biogenesis of the photosystem II inner antenna CP47 (PsbB) in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol, 2009, 149:1076–1086



[14]García-Cerdán J G, Kovács L, Tóth T, Kereïche S, Aseeva E, Boekema E J, Mamedov F, Funk C, Schröder W P. The PsbW protein stabilizes the supramolecular organization of photosystem II in higher plants. Plant J, 2011, 65: 368–381



[15]Massoz S, Larosa V, Horrion B, Matagne R F, Remacle C, Cardol P. Isolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement. J Biotechnol, 2015, 215: 27–34



[16]Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 1989, 990: 87–92



[17]van Kooten O, Snel J F. The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res, 1990, 25: 147–150



[18]Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743



[19]Yoo S D, Cho Y H, Sheen J. Arabaidopsis mesophyll protoplasts: A versatile cell system for transient gene expression analysis. Nat Prot, 2007, 2: 1565–1572



[20]Wang P T, Liu H, Hua H J, Wang L, Song C P. A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis. Chin Sci Bull, 2011, 56: 3538–3546



[21]Sun Q, Emanuelsson O, van Wijk K J. Analysis of curated and predicted plastid subproteomes of Arabidopsis. Subcellular compartmentalization leads to distinctive proteome properties. Plant Physiol, 2004, 135: 723–734



[22]Baker N R. Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol, 2008, 59: 89–113



[23]Murchie E H, Lawson T. Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot, 2013, 64: 3983–3998



[24]Maxwell K, Johnson G N. Chlorophyll fluorescence--a practical guide. J Exp Bot, 2000, 51: 659–668

 


 
[1] Liu En-Bo, Chen Jing, Li Hong-Xing, Yu Ning-Ning, Ren Bai-Zhao, Zhao Bin, Liu Peng, Zhang Ji-Wang. Shading inhibits ear and tassel development of summer maize by altering source-sink balance and regulating carbohydrate metabolism [J]. Acta Agronomica Sinica, 2026, 52(6): 1891-1901.
[2] Jin Yu-He, Wang Xue-Fei, Xu Zhang-Yi-Wa, Miao Yi-Ning, Jiang Yun-Jie, Yi Ying, Miao De-Lin, Zhu Jing-Yi, Zhong Yi-Fan, Chen Ming-Heng, Fang Fang, Liu Peng. Effects of exogenous hormones on chlorophyll fluorescence parameters and the antioxidant enzyme system in soybean leaves under low-temperature stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1817-1829.
[3] Zhao Xiang, Li Jia-Yi, Li Shuang, Han Wen-Hui, Huang Jun-Xia, Yao Xing-Dong, Zhang Hui-Jun, Wang Hai-Ying, Xie Fu-Ti. Effects of high temperature on dry matter accumulation and sugar metabolism in different soybean varieties [J]. Acta Agronomica Sinica, 2026, 52(3): 857-865.
[4] GAO Yuan, LI Xia, WEI Shao-Bo, TIAN Xiao-Hai, ZHOU Wen-Bin. Research advances on plant midday depression of photosynthesis [J]. Acta Agronomica Sinica, 2025, 51(9): 2253-2265.
[5] FAN You-Zhong, WANG Xian-Ling, WANG Zong-Kai, WANG Chun-Yun, WANG Tian-Yao, XIE Jie, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, ZHAO Jie, ZHOU Guang-Sheng. Effects of straw incorporation combined with nitrogen management on photosynthetic efficiency and yield of rapeseed following rice [J]. Acta Agronomica Sinica, 2025, 51(8): 2139-2151.
[6] LI Chun-Mei, CHEN Jie, LANG Xing-Xuan, ZHUANG Hai-Min, ZHU Jing, DU Zi-Jun, FENG Hao-Tian, JIN Han, ZHU Guo-Lin, LIU Kai. Map-based cloning and functional analysis of Dwarf and Tillering 1 (DT1) gene in rice [J]. Acta Agronomica Sinica, 2025, 51(2): 347-357.
[7] WANG Li-Ping, LI Pan, ZHAO Lian-Hao, FAN Zhi-Long, HU Fa-Long, FAN Hong, HE Wei, CHAI Qiang, YIN Wen. Response of senescence characteristics for maize leaves under different plastic mulching and using patterns in oasis irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2025, 51(1): 233-246.
[8] FANG Yu-Hui, QI Xue-Li, LI Yan, ZHANG Yu, PENG Chao-Jun, HUA Xia, CHEN Yan-Yan, GUO Rui, HU Lin, XU Wei-Gang. Effects of high light stress on photosynthesis and physiological characteristics of wheat with maize C4-type ZmPEPC+ZmPPDK gene [J]. Acta Agronomica Sinica, 2024, 50(7): 1647-1657.
[9] LI Shi-Kuan, HONG Hui-Long, FU Jia-Qi, GU Yong-Zhe, SUN Ru-Jian, QIU Li-Juan. Mine the genes of premature yellowing and aging in soybean leaves by BSA-seq combined with RNA-seq technology [J]. Acta Agronomica Sinica, 2024, 50(2): 294-309.
[10] GUO Jia-Xin, YE Yang, GUO Hui-Juan, MIN Wei. Effects and variability analysis of different salt and alkali stresses on the proteome of cotton leaves [J]. Acta Agronomica Sinica, 2024, 50(1): 219-236.
[11] LEI Xin-Hui, LENG Jia-Jun, TAO Jin-Cai, WAN Chen-Xi, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, FENG Bai-Li, WANG Meng, GAO Jin-Feng. Effects of foliar spraying selenium on photosynthetic characteristics, yield, and selenium accumulation of common buckwheat (Fagopyrum esculentum M.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1678-1689.
[12] WU Xiang-Qi, LIU Bo, ZHANG Wei, YANG Xue-Ni, GUO Zi-Yan, LIU Tie-Ning, ZHANG Xu-Dong, HAN Qing-Fang. Effects of wheat-pea intercropping on population photosynthetic characteristics and crops productivity [J]. Acta Agronomica Sinica, 2023, 49(4): 1079-1089.
[13] WANG Zhen, ZHANG Xiao-Li, LIU Miao, YAO Meng-Nan, MENG Xiao-Jing, QU Cun-Min, LU Kun, LI Jia-Na, LIANG Ying. Transcriptional differential expression analysis between BnMAPK1-overexpression and Zhongyou 821 rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2023, 49(3): 856-868.
[14] LI Meng-Yuan, ZHANG Wen-Cheng, GAO Yong, QIN Yong-Tian, BO Shi-Rong, SONG Kun-Yang, TANG Ji-Hua, FU Zhi-Yuan. Map-based cloning and allelic analysis of gene controlling maize kernel mutant crk4 [J]. Acta Agronomica Sinica, 2023, 49(10): 2613-2620.
[15] WANG Quan, WANG Le-Le, ZHU Tie-Zhong, REN Hao-Jie, WANG Hui, CHEN Ting-Ting, JIN Ping, WU LI-Quan, YANG Ru, YOU Cui-Cui, KE Jian, HE Hai-Bing. Effects of HgCl2 on photosynthetic characteristics and its physiological mechanism of rice leaves in vitro feeding [J]. Acta Agronomica Sinica, 2022, 48(9): 2377-2389.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!