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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (8): 1152-1158.doi: 10.3724/SP.J.1006.2018.01152

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Improving Fiber Yield and Quality in the Short Season Cotton Variety Jinmian 11 by Introducing FBP7::iaaM

Xiao-Yan DING(),Juan ZHAO,Shan-Shan QIAN,Xing-Ying YAN,Yan PEI()   

  1. Biotechnology Research Center, Southwest University, Chongqing, 400716, China
  • Received:2017-10-20 Accepted:2018-06-12 Online:2018-08-10 Published:2018-06-19
  • Contact: Yan PEI E-mail:DXYWORK2012@sina.cn;peiyan3@swu.edu.cn
  • Supported by:
    National Major Project of Breeding(2016YFD0100505);the National Major Project for Developing New GM Crops(2016ZX08005-003-004);the National Natural Science Foundation of China(31130039)

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Abstract:

Short season cotton is of particular importance for sustainable development of cotton production in China where has a huge population and limited arable land resources. Usually, early maturity is associated with lower yield and poor quality. We previously developed a novel transgenic cotton material with improved fiber yield and quality by spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells. In this study, we introduced FBP7::iaaM into a short season cotton variety Jinmian 11, which has low lint percentage and high macronaire value, through backcross breeding. A consecutive two-year field experiment indicated that the transgenic-homozygous backcrossed progeny (JBC4) exhibited a significant improvement in fiber yield and fiber fineness. Compared with Jinmian 11, lint percentage and lint yield of JBC4 increased by 12.8% and 56.3%, respectively, and meanwhile, the micronaire value decreased by 10.7%. Our data demonstrate a significant potential of the FBP7::iaaM transgenic in the yield and quality improvement for short season cotton.

Key words: auxin, transgene, short season cotton, backcrosse, lint, macronaire

Fig. 1

PCR amplification of iaaM gene from progenies of backcross M: marker 2000; J11: Jinmian 11; FM: FBP7::iaaM; the rest lanes are progenies of backcross."

Table 1

Segregation of GUS gene in self-crossed progeny of JBC4"

GUS染色
GUS staining		 GUS染色实际株数
Actual number		 理论株数
Theoretical number		 χ2
阳性株数 Positive plant number 129 125.25 0.08
阴性株数 Negative plant number 38 41.75 0.25
总数 Total 167 167.00 0.33

Fig. 2

Comparison of plant height between Jinmian 11 and JBC4"

Fig. 3

Comparison of field phenotype between Jinmian 11 and JBC4"

Fig. 4

Comparison of relative expression of iaaM gene, content of IAA, initial density in 0 DPA ovule and number of mature fibers in Jinmian 11 and JBC4 A: relative expression of iaaM gene; B: the content of IAA in 0 DPA ovule; C: the initial density of fiber in 0 DPA ovule; D: number of mature fibers per seed. J11: Jinmian 11; JBC4: the fourth generation of homozygous progenies of backcross. The significant difference was analyzed by Student’s t-test. * Significant at the 0.05 probability level. ** Significant at the 0.01 probability level."

Table 2

Comparison of fiber quality and yield for J11 and JBC4 in 2014-2015 field trial"

年份
Year		 株系
Line		 马克隆值
Macronaire		 衣分
Lint percentage (%)		 子棉产量
Seed cotton yield (kg plot-1)		 皮棉产量
Lint cotton yield (kg plot-1)
2014 J11 5.28 31.39 3.72 1.17
JBC4 4.61* 39.36** 4.85** 1.90**
2015 J11 5.40 31.15 9.01 2.81
JBC4 4.93** 37.11 ** 11.38* 4.22**

Table 3

Comparison of yield components for J11 and JBC4 in 2014-2015 field trial"

年份
Year		 株系
Line		 单铃重
Boll weight
(g)		 单株结铃数
Number of bolls
per plant		 每铃种子数
Seed number
per boll		 衣指
Lint index
(g)		 子指
Seed index
(g)
2014 J11 3.69 16.80 24.42 4.74 10.36
JBC4 3.99 19.60 26.85 6.01** 9.33**
2015 J11 4.59 32.73 25.79 5.55 12.25
JBC4 5.58* 36.33 33.62** 6.17* 10.45**

Table 4

Comparison of fiber quality and yield for Coker 100 and KBC4 in 2015 field trial"

株系
Line		 马克隆值
Macronaire		 衣分
Lint percentage (%)		 子棉产量
Seed cotton yield (kg plot-1)		 皮棉产量
Lint cotton yield (kg plot-1)
K100 4.93 32.50 9.99 3.25
KBC4 4.63* 34.95** 12.08* 4.24**

Table 5

Comparison of yield components for Coker 100 and KBC4 in 2015 field trial"

株系
Line		 单铃重
Boll weight
(g)		 单株结铃数
Number of bolls
per plant		 每铃种子数
Seed number
per boll		 衣指
Lint index
(g)		 子指
Seed index
(g)
K100 4.43 37.60 23.9 6.03 12.52
KBC4 5.30* 38.13 28.7** 6.44 11.99*
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