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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2059-2069.doi: 10.3724/SP.J.1006.2014.02059


Whole-Genome Sequence Isolation, Chromosome Location and Characterization of Primary Auxin-Responsive Aux/IAA Gene Family in Aegilops tauschii

QIAO Lin-Yi1,4,**,LI Xin1,**,CHANG Zhi-Jian1,ZHANG Xiao-Jun1,ZHAN Hai-Xian1,GUO Hui-Juan1,LI Jian-Bo4,CHANG Jian-Zhong3,*,ZHENG Jun2,*   

  1. 1Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China; 2 Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen 041000, China; 3 Research Center of Dryland Farming, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China; 4Graduate School of Shanxi University, Taiyuan 030006, China
  • Received:2014-05-23 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-20
  • Contact: 常建忠, E-mail: cjzyfx@163.com; 郑军, E-mail: zjsaas@126.com E-mail:qiaoly1988@126.com


Auxin, as one of the most important hormones, plays a key role in many processes of plant development. The Aux/IAA family contains important early auxin response genes. A genome-wide research of Aux/IAA genes in Aegilops tauschii was carried out using bioinformatic method. In this study, 28 Aux/IAA genes were identified in Ae. tauschii, which were distributed on seven chromosomes of Ae. tauschii genome. Twenty Aux/IAA genes share four conserved amino acid sequence motifs and five were mapped on the same locus with known markers. AetIAA3, AetIAA11, and AetIAA26 were specifically expressed in pistil, seed and root of Ae. tauschii, respectively. Eleven pairs of Ae. tauschiiTriticum urartu and five pairs of Ae. tauschiiHordeum vulgare Aux/IAA proteins were orthologous in the phylogenetic tree. Collinearity analysis indicated Aux/IAA genes showed a higher synteny between Ae. tauschii and two other species (Brachypodium distachyon and Oryza stativa). The Aux/IAA genes isolated can not only be applied in genetic improvement of common wheat but also provide basic information in further research of Aux/IAA genes in wheat.

Key words: Aegilops tauschii, Auxin, Aux/IAA gene family, Chromosome location, Bioinformatics

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