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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (11): 1621-1631.doi: 10.3724/SP.J.1006.2015.01621

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Identification and Map-based Cloning of rad-1 and rad-2, Two Rice Architecture Determinant Mutants

NIU Jing,CHEN Sai-Hua*,ZHAO Jie-Yu,ZENG Zhao-Qiong,CAI Mao-Hong,ZHOU Liang,LIU Xi,JIANG Ling,WAN Jian-Min   

  1. Nanjing Agricultural University, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
  • Received:2015-04-25 Revised:2015-07-20 Online:2015-11-12 Published:2015-08-05

Abstract:

Plant architecture is a key element of crop yield and also taken as a criterion in breeding. In this study, two rice architecture determinant mutants, rad-1 and rad-2 were selected from Gamma-radiation induced mutants of rice cultivar Asominori. Each of them shows winding seedlings, dwarf plants and thereby decreased yield. It has been estimated that rad-1 and rad-2 are allelic to each other by allelism test and linkage analysis. After map-based cloning, the responsible gene for the mutations was restricted within a 230 kb region in chromosome 7. Sequence analysis revealed that OsFH5 gene, encoding a forming-like protein altered both in rad-1 and rad-2. An 8-bp deletion in exon 2 in rad-1 and a single nucleotide change in intron 14 in rad-2 made frame-shift reading and produced truncated proteins. OsFH5 gene exhibited constitutive expression in tested tissues. Compared with the wild type, prominent decline of OsFH5 gene was observed in each mutant. In seedlings, loss-function of OsFH5 gene resulted in irregular cell growth in leaf sheath and shorter cells in inner husk, which therefore caused short grain and lower grain weight. By quantitative RT-PCR analysis, several auxin response factors were dramatically down-regulated in rad-2, which suggested that OsFH5 may affect the response to auxin in rice.

Key words: Rice, Architecture, Mutant, Map-based cloning, Auxin response factor

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