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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (9): 1779-1790.doi: 10.3724/SP.J.1006.2021.04151

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Regulation of carbon and nitrogen metabolism in leaf of soybean cultivar Suinong 26 at seed-filling stage under drought stress by exogenous melatonin

CAO Liang(), DU Xin, YU Gao-Bo, JIN Xi-Jun, ZHANG Ming-Cong, REN Chun-Yuan, WANG Meng-Xue*(), ZHANG Yu-Xian*()   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Received:2020-07-10 Accepted:2021-01-21 Online:2021-09-12 Published:2021-03-01
  • Contact: WANG Meng-Xue,ZHANG Yu-Xian E-mail:miss9877@126.com;wangmengxue1978@163.com;zyx_lxy@126.com
  • Supported by:
    National Key Research and Development Program of China “Physiological Basis and Agronomic Management for High-quality and High-yield of Field Cash Crops”(2018YFD1000905);Heilongjiang Application Technology Research and Development Projects(GA19B101-02);Heilongjiang Provincial Land Reclamation Bureau Key Research Project(HKKY190206-01)

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Abstract:

The grain-filling stage is the most complex stage of carbon and nitrogen metabolism. Drought stress inevitably inhibits the assimilation, distribution, and transition of carbon and nitrogen at grain-filling stage in soybean, resulting in less soybean yield. The objective of this study was to investigate the effects of exogenous melatonin on the carbon and nitrogen metabolism genes and pathways under drought stress in soybean. Transcriptome analysis showed that, compared with drought stress treatment, 37 and 493 genes were jointly up-regulated and down-regulated in soybean leaves treated with normal water supply and treated with exogenous melatonin under drought stress, respectively. The up-regulated genes included functional genes directly and indirectly involved in carbon and nitrogen metabolism, such as the key genes involved in the cysteine synthesis pathway, photosynthesis, carbohydrate metabolism, and glucose metabolism. Metabolomic analysis revealed that, compared with drought stress treatment, 17 and 43 metabolites were jointly up-regulated and down-regulated in soybean leaves treated with normal water supply and treated with exogenous melatonin under drought stress, respectively. Most (14/17) of up-regulated metabolites were amino acids, lipids, organic acids, and carbohydrates, which further indicated that exogenous melatonin could improve soybean carbon and nitrogen metabolism and drought resistance in soybean. Combined with transcriptome and metabolomic profile, melatonin promoted the relative expression level of β-D-Glucosidase gene due to regulate the pathway of amino acid metabolism and starch and sucrose metabolism, improved the contents of L-Asparagine and D-glucose-6P metabolites, and ultimately improves the ability of drought resistance in soybean.

Key words: melatonin, soybean, drought at grain-filling stage, carbon and nitrogen metabolism, metabolome and transcriptome

Fig. 1

Comparisons of differentially expressed genes in WW/D and DM/D Grey dots represent genes without significant differential expressions, red and blue dots denote significantly up-regulated and down-regulated genes in the WW/D and DM/D comparisons, respectively. WW is the normal water supply group, D is the drought stress group, and DM is the foliar application of melatonin under drought stress group."

Fig. 2

Effects of exogenous melatonin on leave transcriptome under drought stress in soybean A: Venn diagram of the differentially expressed genes between WW/D and DM/D. B: the heat maps of the common differentially expressed genes between WW/D and DM/D. Treatments are the same as those given in Fig. 1. "

Fig. 3

GO biological process enrichment of up-regulated differentially expressed genes under melatonin treatment in WW/D and DM/D A: the heatmap of the up-regulated expressed genes between the WW/D and DM/D. B: GO biological process enrichment on common up-regulated expressed genes between the WW/D and DM/D. Treatments are the same as those given in Fig. 1. "

Fig. 4

KEGG pathway enrichment for differentially up-regulated and down-regulated expressed genes both in WW/D and DM/D A: up-regulated. B: down-regulated. The rich factor is calculated as the differentially expressed genes number divided by the base number of any given pathway. Dot size denotes the number of genes and dot color denotes the range of adjusted P-value. Treatments are the same as those given in Fig. 1. "

Fig. 5

RT-PCR validation of relative expression levels of DEGs White bar signifies transcriptomic data of the differentially expressed genes, and black bar denotes qRT-PCR results of the differentially expressed genes. Treatments are the same as those given in Fig. 1. "

Fig. 6

PCA analysis of metabolomics Treatments are the same as those given in Fig. 1. "

Fig. 7

Effects of drought stress and exogenous melatonin treatment on the metabolome in soybean A: the venn diagram shows the overlapped differentially-accumulated metabolites between the WW/D and DM/D comparisons. B: the histogram of KEGG pathway enrichment for differentially-accumulated metabolites both in WW/D and DM/D comparison. X-axis is the number of differentially expressed genes. Treatments are the same as those given in Fig. 1. "

Fig. 8

KEGG pathway of starch sucrose metabolism and cyanoamino acid metabolism A: starch and sucrose metabolism. B: cyanoamino acid metabolism. Red and pink denote up-regulated metabolites and genes both in WW/D and DM/D."

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