花生全基因组抗病基因鉴定及其对青枯菌侵染的响应分析

doi:10.3724/SP.J.1006.2021.04266

Abstract

Abstract:

Peanut is one of the main oil crops, which is harmed by many pathogenic microorganisms during growth and development period. Breeding and selection of disease-resistant varieties is one of the most economical and effective ways to control disease, and disease resistance genes are important genes for plant resistance to pathogenic microorganisms. Here, the whole genome-wide identification of peanut disease resistance genes was carried out for the first time. A total of 4156 candidate disease resistance genes were identified. Among them, 536, 490, 232, 182, and 149 genes were RLK, RLP, NL, CNL, and TNL, respectively. The distribution of disease resistance genes was uneven on chromosomes, and most of them were concentrated on chromosome B02. Transcriptome profiling revealed that 111 genes were specifically expressed in resistant materials, 104 genes were specifically expressed in susceptible materials, 2216 genes were expressed in both resistant and susceptible materials, while 1725 genes were not expressed in both resistant and susceptible materials. Two kinds of differentiate expressed R genes were identified, including five genes in the first group responded to the infection of Ralstonia solanacearum at specific time and 65 genes in the second group which exhibited higher expressions in resistant cultivar than susceptible cultivar. A candidate gene Arahy.5D95TJ was successfully validated by qRT-PCR. In this study, the identification and analysis of peanut disease resistance genes provides the important reference for further research of their functions and molecular breeding of peanut disease resistance.

Key words: Arachis hypogaea, disease resistance gene, bacterial wilt, transcriptome profiling

ZHANG Huan, LUO Huai-Yong, LI Wei-Tao, GUO Jian-Bin, CHEN Wei-Gang, ZHOU Xiao-Jing, HUANG Li, LIU Nian, YAN Li-Ying, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Genome-wide identification of peanut resistance genes and their response to Ralstonia solanacearum infection[J].Acta Agronomica Sinica, 2021, 47(12): 2314-2323.

Figures/Tables 5

Table 1

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类别 Type	包含结构域 Domain contained	基因数 Gene number	占R基因比例 Proportion in R gene (%)
RLK	TM, LRR, Kinase	536	12.90
RLP	TM, LRR	490	11.79
CNL	CC, TM, NBS, LRR	182	4.38
TNL	TM, TIR, NBS, LRR	149	3.59
KIN	TM, Kinase	1714	41.24
NL	TM, NBS, LRR	232	5.58
CK	CC, TM, Kinase	221	5.32
N	TM, NBS	146	3.51
CN	CC, TM, NBS	136	3.27
CTNL	CC, TM, TIR, NBS, LRR	77	1.85
L	LRR	76	1.83
T	TM, TIR	65	1.57
TN	TM, TIR, NBS	61	1.47
CL	CC, TM, LRR	28	0.67
CLK	CC, TM, LRR, Kinase	20	0.48
CNT	CC, TM, NBS, TIR	15	0.36
CT	CC, TM, TIR	6	0.14
TRAN	TM	2	0.05
总计Total		4156

Genome-wide identification of peanut resistance genes and their response to Ralstonia solanacearum infection

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