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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (2): 478-487.doi: 10.3724/SP.J.1006.2022.11026

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Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid

CHEN Xin-Yi1(), SONG Yu-Hang1, ZHANG Meng-Han1, LI Xiao-Yan1, LI Hua1, WANG Yue-Xia1,*(), QI Xue-Li2,*()   

  1. 1College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Institute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2021-03-12 Accepted:2021-07-12 Online:2022-02-12 Published:2021-08-03
  • Contact: WANG Yue-Xia,QI Xue-Li E-mail:xychen3988@163.com;yxwang2100@126.com;xueliqi888@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(U1704103);the National Key Research and Development Program of China(2015CB150106)

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Abstract:

Drought stress happens frequently in Huang-Huai winter wheat planted area, causing severe injury on photosynthetic apparatus of wheat seedlings. To characterize the traits of photosynthetic physiology in different wheat cultivars in response to water deficit, the newly in-lab cultivated Zhengmai 1860, as well as Bainong 207 and Zhoumai 18 were used as materials in this study. We explored the effects of water deficit on photosynthetic traits, antioxidant enzyme activities, and related gene transcription of seedlings in different cultivars, as well as the drought alleviation effect of exogenous application of 5-aminolevulinic acid (ALA). The results were as follows: Zhengmai 1860 had comparatively higher root dry weight and root shoot ratio than the other two cultivars under drought stress. Compared to Zhoumai 18, the drought resistant cultivars (Zhengmai 1860 and Bainong 207) had lower reduction in chlorophyll content and increased MDA content, enhanced the activities of SOD and CAT, and lowered reductions in chlorophyll fluorescence parameters and the photosynthetic parameters. Meanwhile, water deficit obviously improved the transcriptions of antioxidant enzyme-encoded genes CAT, SOD-Cu/Zn, MnSO, and FeSOD, which showed a correlation between the increasing level with the drought resistant ability. The exogenous pretreatment of ALA further enhanced the activities of SOD and CAT to lower the damage to membrane lipid peroxidation by inducing the transcriptions of CAT, SOD-Cu/Zn, and MnSOD. ATPase activity was also increased to alleviate water deficit on the damage to photosynthetic physiology. Moreover, we found for the first time that the transcriptional maintenance of chloroplast photosynthetic apparatus related psb28 gene had a correlation with the drought resistance between different wheat cultivars, which was also significantly induced by the exogenously pretreated ALA. These results in this study proposed that the transcriptions of antioxidant enzymes and chloroplast photosynthesis related genes had a close relationship with the drought resistant ability of wheat and the alleviation effect of exogenous ALA in wheat.

Key words: wheat, water deficit, cultivar, gene expression, 5-aminolevulinic acid

Table1

Primer information for qRT-PCR"

基因名称
Gene name		 基因功能
Gene function		 引物序列
Primer sequence (5′-3′)		 参考文献
Reference
SOD-Cu/Zn 叶绿体Cu和Zn超氧化物歧化酶
Chloroplast Cu/Zn superoxide dismutase		 F: CGCTCAGAGCCTCCTCTTT
R: CTCCTGGGGTGGAGACAAT		 [19]
FeSOD 叶绿体Fe超氧化物歧化酶
Chloroplast Fe superoxide dismutase		 F: GTCCTACTACGGCCTCACCA
R: ACGTAGTCCTGCTGGTGCTT		 [20]
MnSOD 线粒体Mn超氧化物歧化酶
Mitochondrion Mn superoxide dismutase		 F: CAGAGGGTGCTGCTTTACAA
R: GGTCACAAGAGGGTCCTGAT		 [20]
CAT 过氧化氢酶
Catalase		 F: CCATGAGATCAAGGCCATCT
R: ATCTTACATGCTCGGCTTGG		 [19]
psb28 叶绿体Psb28蛋白
Chloroplast Psb28 protein		 F: AACTGTCGAGCTGGTAACGG
R: ACCGGTTTTCCTCAGTTCGT		 本研究设计
Design in this study
Actin β-actin F: GGAATCCATGAGACCACCTAC
R: GACCCAGACAACTCGCAAC		 [21]

Fig. 1

Effects of drought stress and exogenous ALA on the appearance and biomass of different drought-resistant wheat cultivar Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: pretreatment with ALA prior to drought stress."

Fig. 2

Effects of drought stress and exogenous ALA on the chlorophyll and MDA contents, and the activities of antioxidant enzymes for different drought-resistant wheat cultivars Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: exogenous ALA pretreatment prior to water deficit treatment."

Fig. 3

Effects of drought stress and exogenous ALA on the chlorophyll fluorescence parameters and the photosynthetic parameters of different drought-resistant wheat cultivar Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: exogenous ALA pretreatment prior to water deficit treatment."

Fig. 4

Effects of drought stress and exogenous ALA on ATPase activity of different drought-resistant wheat cultivar Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: exogenous ALA pretreatment prior to water deficit treatment."

Fig. 5

Effects of drought stress and exogenous ALA on the transcriptions of antioxidant enzyme-related genes of different drought-resistant wheat cultivar Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: exogenous ALA pretreatment prior to water deficit treatment."

Fig. 6

Effects of drought stress and exogenous ALA on the transcription of chloroplast psb28 gene in different drought- resistant wheat cultivar Data are means ± standard deviations of three replicates. Different letters indicate significant difference among treatments within each cultivar at P < 0.05. CK: normal water supply; Drought: water deficit treatment; D+A: exogenous ALA pretreatment prior to water deficit treatment."

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