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作物学报 ›› 2018, Vol. 44 ›› Issue (10): 1477-1484.doi: 10.3724/SP.J.1006.2018.01477

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻高秆染色体片段代换系Z1377的鉴定及重要农艺性状QTL定位

崔国庆1,王世明1,马福盈1,汪会1,向朝中2,李云峰1,何光华1,张长伟1,杨正林1,凌英华1,赵芳明1,*()   

  1. 1 西南大学水稻研究所/西南大学农业科学研究院, 重庆 400715
    2 重庆市丰都县龙孔镇农业服务中心, 重庆408200
  • 收稿日期:2018-01-05 接受日期:2018-06-12 出版日期:2018-10-10 网络出版日期:2018-06-20
  • 通讯作者: 赵芳明
  • 基金资助:
    本研究由国家重点研发计划项目(2017YFD0100202);重庆市科委主题专项(CSTC, 2016shms-ztzx0017);西南大学基本业务费专项创新团队项目(XDJK2017A004)

Identification of Rice Chromosome Segment Substitution Line Z1377 with Increased Plant Height and QTL Mapping for Agronomic Important Traits

Guo-Qing CUI1,Shi-Ming WANG1,Fu-Ying MA1,Hui WANG1,Chao-Zhong XIANG2,Yun-Feng LI1,Guang-Hua HE1,Chang-Wei ZHANG1,Zheng-Lin YANG1,Ying-Hua LING1,Fang-Ming ZHAO1,*()   

  1. 1 Rice Research Institute, Southwest University / Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
    2 Center of Agricultural Serve at Longkong Town, Fengdu County, Chongqing 408200, China
  • Received:2018-01-05 Accepted:2018-06-12 Published:2018-10-10 Published online:2018-06-20
  • Contact: Fang-Ming ZHAO
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2017YFD0100202);the Project of Chongqing Science & Technology Commission(CSTC, 2016shms-ztzx0017);the Fundamental Research Funds for the Central Universities(XDJK2017A004)

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摘要：

株高是水稻重要的农艺性状, 往往与产量相关性状密切关联, 在水稻育种中有重要利用价值。本研究以日本晴为受体、缙恢35为供体亲本, 经表型和分子标记双重选择, 鉴定了一个水稻高秆染色体片段代换系Z1377。Z1377共含有18个代换片段, 平均代换长度为2.95 Mb。与日本晴相比, Z1377的株高、倒一节间至倒四节间长、穗长、一次枝梗数、二次枝梗数、粒长、每穗实粒数、总粒数显著增加; 粒宽显著变细, 有效穗数、结实率显著减少, 但仍达86.75%。用日本晴与Z1377杂交构建的次级F2群体共检测到16个相关QTL, 分布于第2、第3、第4、第5、第6、第7和第9染色体。其中有8个可能与已克隆基因等位, 如GW2、EUI1、ZFP185等, 另8个如qPH3等尚未见报道。Z1377的株高由一个主效QTL (qPH3)和一个微效QTL (qPH5)控制, 其中qPH3的贡献率达28.59%。而且, 在F2群体中, 高秆和矮秆基本呈现双峰分布, 经卡平方测验, 符合3∶1分离比, 表明高秆对矮秆显性, 并主要由qPH3负责。这将为该主效基因的精细定位和克隆奠定基础, 同时为进一步选育含2~3个代换片段的中高优良染色体片段代换系并应用于育种奠定基础。

关键词: 水稻, 染色体片段代换系, 株高, 产量相关性状, QTL定位

Abstract:

Plant height is an important agronomic trait in rice, usually relating to yield-related traits. Here, a novel rice chromosome segment substitution line Z1377 with increased plant height was identified from recipient Nipponbare and donor Jinhui 35 through selection of both phenotype and molecular marker. Z1377 carried 18 substitution segments with average substitution length of 2.95 Mb. Compared with Nipponbare, Z1377 had significantly increased plant height, length of the 1st, 2nd, 3rd, and 4th internode, panicle length, number of primary and secondary branches and grain length, and decreased grain width, number of panicles per plant and seed setting ratio. However, the seed setting ratio was still 86.75%. Furthermore, F2 population from crosses between Nipponbare and Z1377 was used to map QTLs for plant height and other important agronomic traits. A total of 16 QTLs were detected, of which eight had been reported with the cloned genes such as GW2, EUI1, ZFP185, and the other eight were still not reported, such as qPH3. The plant height of Z1377 was mainly controlled by a major QTL (qPH3) with the explained phenotypic variance of 28.59% and a minor QTL (qPH5). Moreover, the high and dwarf plants basically displayed a bimodal distribution in the F2 population, and fitted to 3:1 segregation ratio by Chi-square test, indicating that high plant is dominant to dwarf plants and mainly conferred by qPH3. These results lay a foundation for fine mapping and cloning qPH3, meanwhile, also provide good bases for developing excellent chromosome segment substitution lines with moderate height plant carrying 2-3 substitution segments to be used in breeding.

Key words: rice, chromosome segment substitution lines, plant height, yield-related traits, QTL mapping

图1

Z1377的代换片段 每条染色体左侧为物理距离(Mb)和定位的QTL; 右侧为标记名称、代换区间(框内标记)和代换长度(黑箭头指向)。"

表1

成熟期的日本晴和Z1377及F2群体各性状统计参数"

性状
Trait		 平均值±标准差(亲本)
Mean±SD (parents)		 F2 群体
F2 population
日本晴
Nipponbare		 Z1377 平均值±标准差
Mean±SD		 范围
Range		 偏度
Skewness		 峰度
Kurtosis
株高Plant height (cm) 89.68±2.65** 171.39±3.87** 130.07±21.39 81.60-169.50 -0.62 -0.58
有效穗数Panicle number 13.65±3.30** 5.20±1.17** 8.70±4.17 1.00-27.00 1.24 3.97
倒一节Length of the first internode (cm) 39.02±2.16** 56.02±1.81** 45.69±6.23 32.00-59.80 -0.17 -0.65
倒二节Length of the second internode (cm) 18.32±1.37** 26.58±1.27** 22.45±4.71 10.50-32.30 -0.36 -0.03
倒三节Length of the third internode (cm) 10.19±1.67** 22.45±1.64** 15.96±4.60 3.80-26.20 -0.21 -0.12
倒四节Length of the fourth internode (cm) 1.84±0.75** 17.46±1.05** 11.96±3.54 1.80-19.50 -0.89 1.05
倒五节Length of the fifth internode (cm) — 11.08±2.78 7.03±3.20 1.70-13.70 0.20 -0.88
倒六节Length of the sixth internode (cm) — 4.00±1.29 5.21±3.00 1.30-10.10 0.15 -1.52
穗长Panicle length (cm) 20.09±2.17** 33.49±3.17** 27.86±5.39 18.00-42.77 0.22 -0.32
一次枝梗数Number of primary branch 8.43±1.73** 15.33±1.91** 12.12±2.63 7.21-18.10 -0.03 -0.56
二次枝梗数Number of secondary branch 26.47±7.51** 71.69±22.46** 49.85±17.90 21.13-98.90 0.32 -0.29
粒长Grain length (mm) 7.01±0.10** 10.02±0.16** 8.45±0.75 7.00-9.87 -0.35 -0.85
粒宽Grain width (mm) 3.42±0.07** 2.92±0.08** 3.14±0.25 2.50-3.60 -0.41 -0.62
实粒数Grains per panicle 96.99±8.62** 233.06±55.96** 91.69±53.23 2.00-224.00 0.28 -0.44
总粒数Spikelets per panicle 103.04±8.82** 272.65±69.96** 187.18±71.76 42.00-370.00 0.39 -0.25
结实率Seed setting ratio (%) 94.13±0.01** 86.75±2.97** 0.54±0.31 0.02-0.95 -0.29 -1.42
千粒重1000-grain weight (g) 25.60±0.56 25.37±0.66 24.61±3.21 16.60-31.40 -0.26 -0.44
单株产量Yield per plant (g) 33.82±7.15 30.77±7.43 22.42±17.71 0.27-82.4 1.16 1.62

图2

日本晴和Z1377的表型"

图3

日本晴与Z1377杂交的F2群体株高分布"

表2

水稻重要农艺性状QTL"

性状
Trait		 QTL 染色体
Chr.		 连锁标记
Linked marker		 估计效应
Estimated effect		 贡献率
Var. (%)		 P值
P-value
株高Plant height (cm) qPH3 3 RM14412 7.52 28.59 0.0008
株高Plant height (cm) qPH5 5 RM3321 4.65 10.96 0.0305
倒一节Length of the first internode (cm) qLFI2 2 RM6378 1.15 3.65 0.0474
倒三节Length of the third internode (cm) qLTI4 4 RM7187 1.19 8.50 0.0367
倒三节长Length of the third internode (cm) qLTI6-1 6 RM3183 1.80 19.41 0.0031
倒三节长Length of the third internode (cm) qLTI6-2 6 RM103 1.13 7.59 0.0484
倒四节长Length of the fourth internode (cm) qLFOI3 3 RM14412 1.31 16.44 0.0043
穗长Panicle length (cm) qPL2 2 RM6378 1.30 11.00 0.0124
一次枝梗数Number of primary branch qNPB2 2 RM6378 0.54 8.09 0.0188
二次枝梗数Number of secondary branch qNSB2 2 RM6378 4.57 11.09 0.0082
粒长Grain length (mm) qGL3 3 RM14412 0.18 12.38 0.0220
粒长Grain width (mm) qGW2 2 RM6378 -0.06 7.43 0.0210
实粒数Grains per panicle qGPP6 6 RM3183 38.86 0.50 0.0062
实粒数Grains per panicle qGPP9 9 RM7048 90.26 2.71 0.0025
总粒数Spikelets per panicle qSPP2 2 RM6378 14.68 5.82 0.0433
有效穗数Panicle number qPN7 7 RM5481 -2.53 3.90 0.0293
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