作物学报 ›› 2019, Vol. 45 ›› Issue (1): 46-54.doi: 10.3724/SP.J.1006.2019.82022
Sai-Sai XIA,Yu CUI,Feng-Fei LI,Jia TAN,Yuan-Hua XIE,Xian-Chun SANG,Ying-Hua LING()
摘要:
经甲基磺酸乙酯(EMS)诱变优良籼型水稻恢复系缙恢10号, 获得一个稳定遗传的水稻类病斑早衰突变体lmps1 (lesion mimic and premature senescence 1)。该突变体苗期表型正常, 分蘖早期出现褐色类病斑, 且斑点数目随植株生长而增多, 孕穗期叶片开始萎黄衰老。与野生型相比, 突变体lmps1的每穗总粒数下降8% (P<0.05), 株高、穗长、有效穗数、每穗实粒数、结实率以及千粒重分别下降14.3%、24.3%、27.2%、50%、45.7%与14.5%, 差异均达极显著水平(P<0.01)。遮光处理表明, 突变体lmps1的类病斑性状受光照诱导。孕穗期叶片光合色素含量下降且光合效率降低, H2O2含量增加, 抗氧化酶SOD和CAT的活性显著降低。透射电镜观察结果显示, 突变体lmps1叶肉细胞中叶绿体数目减少, 叶绿体的类囊体片层结构损伤降解。qRT-PCR结果显示, 突变体lmps1中防卫反应相关基因除POX22.3表达量降低外, POC1、PAL、PBZ1、PR1、NPR1、PR5表达量均极显著高于野生型。遗传分析表明突变体lmps1的类病斑早衰性状受1对隐性核基因控制, 利用西农1A与突变体lmps1杂交所得F2群体中的突变株, 将目标基因定位于第7染色体长臂端粒附近约167.3 kb的物理区段内。
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