作物学报 ›› 2019, Vol. 45 ›› Issue (3): 477-482.doi: 10.3724/SP.J.1006.2019.84035
• 研究简报 • 上一篇
Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG(),Bing-Guang XIAO()
摘要:
由链格孢菌(Alternaria alternata)引起的赤星病是最具破坏性的烟草叶斑病害之一, 在中国严重地影响烟草(Nicotiana tabacum L.)的产量和质量。选育抗烟草赤星病的优良品种虽是预防该病最经济、有效的途径, 但因其抗性受数量性状基因控制而很难通过常规育种手段实现。为了便于开展烟草抗赤星病分子标记辅助选择育种, 本研究利用抗赤星病雪茄烟品种Beinhart1000-1和感病烤烟品种红花大金元经杂交、自交产生的362个F2单株构建了一张包含670个SSR标记的烟草遗传连锁图谱, 并结合组培快繁形成的F2株系的田间赤星病病情指数(DI), 在全基因组范围内检测获得2个与烟草赤星病抗性相关的QTL, 分别位于第20和23连锁群上的SSR标记TMs05179和TMs04022, 以及TM61049和TM62212之间。这2个QTL等位基因均来自抗病亲本Beinhart1000-1, 它们一起解释了两亲本间81%的病情指数(DI)差异及64%的加性效应。为下一步开展烟草抗赤星病的分子标记辅助育种奠定了基础。
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