欢迎访问作物学报,今天是

作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1596-1602.doi: 10.3724/SP.J.1006.2017.01596

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用MR1523/苏御糯F2:3群体定位水稻抗灰飞虱QTL

仲杰1,温培正1,孙志广1,肖世卓1,胡金龙1,张乐1,江玲1,程遐年1,刘裕强1,*,万建民1,2   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室,江苏南京 210095;2中国农业科学院作物科学研究所,北京 100081
  • 收稿日期:2017-04-18 修回日期:2017-07-23 出版日期:2017-11-12 网络出版日期:2017-08-11
  • 通讯作者: 刘裕强, E-mail: yql@njau.edu.cn
  • 基金资助:

    本研究由国家重点研发计划项目(2017YFD0100400-01),国家自然科学基金项目(31522039, 31471470)和江苏省自然科学基金项目(BK20150026)资助。

Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population

ZHONG Jie1,WEN Pei-Zheng1,SUN Zhi-Guang1,XIAO Shi-Zhuo1,HU Jin-Long1,ZHANG Le1,JIANG Ling1,CHENG Xia-Nian1,LIU Yu-Qiang1,*,WAN Jian-Min1,2   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-04-18 Revised:2017-07-23 Published:2017-11-12 Published online:2017-08-11
  • Contact: 刘裕强, E-mail: yql@njau.edu.cn
  • Supported by:

    This study was supported by the National Key R&D Program of China (2017YFD0100400-01), National Natural Science Foundation of China (31522039, 31471470), and the Natural Science Foundation of Jiangsu Province of China (BK20150026)

摘要:

灰飞虱是我国水稻生产的主要害虫之一,不仅直接取食危害水稻,还是水稻主要病毒病的传播介体,严重制约水稻生产。籼稻品种MR1523对灰飞虱表现较强的排趋性。为发掘抗灰飞虱新基因,本研究利用MR1523与感虫粳稻品种苏御糯构建了一个包含200个家系的F2:3分离群体,进行灰飞虱抗性鉴定。并利用120对均匀分布在水稻12条染色体的多态性SSR标记,构建了全基因组连锁图谱,进行抗灰飞虱QTL定位。结果分别在水稻第2、第5和第6染色体上检测到Qsbph2、Qsbph5a、Qsbph5b和Qsbph6 4个抗灰飞虱QTLs,分别位于分子标记RM526–RM3763、RM17804–RM13、RM574–RM169和RM190–RM510之间,LOD值分别为2.14、3.13、3.23和2.35,贡献率分别为12.0%、14.7%、17.4%和14.1%,各QTL的抗性等位基因效应均来自抗虫亲本MR1523。该结果为后续抗灰飞虱基因的精细定位及通过分子标记辅助选择培育抗灰飞虱水稻新品种奠定了基础。

关键词: 水稻, 灰飞虱, 抗性, 数量性状基因座

Abstract:

The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacide), is one of the most destructive insect pests in rice (Oryza sativa L.) production. SBPH not only causes direct damage by sucking plant sap but also transmits the main viral diseases, which seriously threatens the safety of rice production. An indica cultivar MR1523 displayed strong antixenosis against SBPH. In order to identify SBPH resistance genes, a F2:3 population derived from a cross between MR1523 and susceptible japonica cultivar Suyunuo was constructed, and then evaluated for SBPH resistance. Moreover, 120 polymorphic SSR markers between the parents uniformly distributed on 12 chromosomes of rice were used to construct the molecular linkage map, and then conducted the QTL assay for SBPH resistance. A total of four QTLs on chromosome 2, 5, and 6, Qsbph2, Qsbph5a, Qsbph5b, and Qsbph6 were detected in the interval of RM526–RM3763, RM17804–RM13, RM574–RM169, and RM190–RM510, with LOD score of 2.14, 3.13, 3.23, and 2.35, and explained the phenotypic variance of 12.0%, 14.7%, 17.4%, and 14.1% , respectively. All of the QTLs came from the resistance parent MR1523. The results in this study lay a foundation for furtherly fine mapping SBPH resistance and developing new cultivars by molecular marker assisted selection in the future.

Key words: Rice (Oryza sativa L.), Small brown planthopper (SBPH);Resistance, Quantitative trait locus (QTL)

[1] Brar D S, Virk P S, Jena K K, Khush G S. Breeding for resistance to planthoppers in rice. In: Heong K L, Hardy B, eds. Planthoppers: new threats to the sustainability of intensive rice production systems in Asia. International Rice Research Institute, Los Ba?os, Philippines, 2009. pp 401–428 [2] 邰德良, 李瑛, 梅爱中, 丁志宽, 王春兰, 仲凤翔. 2004年稻田灰飞虱重发原因分析与控制对策. 中国植保导刊, 2005, 25(3): 29–31 Tai D L, Li Y, Mei A Z, Ding Z K, Wang C L, Zhong F X. Analysis and control measures of rice brown planthopper occurrence in 2004. China Plant Prot, 2005, 25(3): 29–31 (in Chinese) [3] 顾伯良, 薛萍霞, 施文贤, 周丽花. 水稻灰飞虱转移穗部为害及其对产量损失的观察. 中国植保导刊, 2005, 25(5): 7–8 Gu B L, Xue P X, Shi W X, Zhou L H. Observation on rice spikes infected by Laodelphax striatellus and rice yield loss. China Plant Prot, 2005, 25(5): 7–8 (in Chinese with English abstract) [4] 兰莹, 周彤, 范永坚, 周益军. 水稻对灰飞虱传播的两种病毒病抗性的研究进展. 江苏农业学报, 2012, 28: 1480–1486 Lan Y, Zhou T, Fan Y J, Zhou Y J. Advances in rice resistance to two virus diseases carried by small brown planthopper. Jiangsu J Agric Sci, 2012, 28: 1480–1486 (in Chinese with English abstract) [5] Duan C X, Wan J M, Zhai H Q, Chen Q, Wang J K, Su N, Lei C L. Quantitative trait loci mapping of resistance to Laodelphax striatellus (Homoptera: Delphacidae) in rice using recombinant in bredlines. J Econ Entomol, 2007, 100: 1450–1455 [6] 王宝祥, 江玲, 陈亮明, 卢百关, 王琦, 黎光泉, 樊继伟, 程遐年, 翟虎渠, 徐大勇, 万建民. 水稻黑条矮缩病抗性资源的筛选和抗性QTL的定位. 作物学报, 2010, 36: 1258–1264 Wang B X, Jiang L, Chen L M, Lu B G, Wang Q, Tuyen L Q, Fan J W, Cheng X N, Zhai H Q, Xu D Y, Wan J M. Screening of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL. Acta Agron Sini, 2010, 36: 1258–1264 (in Chinese with English abstract) [7] 段灿星, 张世贤, 陈青, 程治军, 翟虎渠, 万建民. 水稻种质资源抗灰飞虱评价及抗性机制分析. 中国水稻科学, 2007, 21: 425–430 Duan C X, Zhang S X, Chen Q, Chen Z J, Zhai H Q, Wan J M. Evaluation of rice germplasm for resistance to the small brown planthopper and analysis on resistance mechanism. Chin J Rice Sci, 2007, 21: 425–430 (in Chinese with English abstract) [8] Sone S, Hattori Y, Tsuboi S, Otsu Y. Difference in susceptibility to imidacloprid of the populations of the small brown planthopper, Laodelphax striatellus Fallén. J Pestic Sci, 1995, 20: 541–543 [9] Endo S, Tsurumachi M. Insecticide resistance and insensitive acetylcholinesterase in small brown planthopper, Laodelphax striatellus. J Pestic Sci, 2000, 25: 395–397 [10] Endo S, Takahashi A, Tsurumachi M. Insecticide susceptibility of the small brown planthopper, Laodelphax striatellus Fallén (Homoptera: Delphacidae), collected from East Asia. Appl Entomol Zool, 2002, 37(1): 79–84 [11] 段灿星, 程治军, 雷才林, 翟虎渠, 万建民. 利用Mudgo/武育粳3号F2群体分析水稻抗灰飞虱QTL. 作物学报, 2009, 35: 388–394 Duan C X, Cheng Z J, Lei C L, Zhai H Q, Wan J M. Analysis of QTLs for resistance to small brown planthopper in rice using an F2 population from across between Mudgo and Wuyujing 3. Acta Agron Sini, 2009, 35: 388–394 (in Chinese with English abstract) [12] Duan C X, Su N, Cheng Z J, Lei C L, Wang J L, Zhai H Q, Wan J M. QTL analysis for the resistance to small brown planthopper (Laodelphax striatellus Fallén) in rice using back cross inbred lines. Plant Breed, 2010, 129: 63–67 [13] 朱文银, 朱其松, 张洪瑞, 陈峰, 陈博聪, 杨连群. 不同类型水稻品种对灰飞虱抗性的初步鉴定. 江苏农业科学, 2012, 40(7): 110–111 Zhu W X, Zhu Q S, Zhang H R, Chen F, Chen B C, Yang L Q. Preliminary identification of resistance to brown planthopper in different rice varieties. J Agric Sci Jiangsu, 2012, 40(7): 110–111 (in Chinese) [14] 孙黛珍, 江玲, 张迎信, 程遐年, 王春明, 翟虎渠, 万建民. 8个水稻品种的条纹叶枯病抗性特征. 中国水稻科学, 2006, 20: 219–222 Sun D Z, Jiang L, Zhang Y X, Cheng X N, Wang C M, Zhai H Q, Wan J M. Resistance to rice stripe in eight rice varieties. Chin J Rice Sci, 2006, 20: 219–222 (in Chinese with English abstract) [15] 刘芳, 宋英, 包善微, 卢海燕, 祝树德, 梁国华. 水稻品种对灰飞虱的抗性及其机制. 植物保护学报, 2007, 34: 449–454 Liu F, Song Y, Bao S W, Lu H Y, Zhu S D, Liang G H. Resistance to small brown planthopper and its mechanism in rice varieties. Acta Phyto Sin, 2007, 34: 449–454 (in Chinese with English abstract) [16] IRRI. Standard evaluation systems for rice. Manila, Philippines: IRRI, 1988 [17] Meng L, Li H H, Zhang L Y, Wang J K. QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015, 3: 269–283 [18] Sarao P S, Bentur J S. Antixenosis and tolerance of rice genotypes against brown planthopper. Rice Sci, 2016, 23: 96–103 [19] 程正新, 黄所生, 黄凤宽, 吴碧球, 韦素美. 水稻品种资源抗白背飞虱核心种质的研究. 中国农学通报, 2014(4): 288–292 Cheng Z X, Huang S S, Huang F K, Wu B Q, Wei S M. Studies on core collection of rice germplasms for resistance to white-backed planthopper. Chin Agric Sci Bull, 2014: 288–292 (in Chinese with English abstract) [20] 邓伟, 胡兰香, 陈红萍, 陈明亮, 肖叶青. 水稻抗白背飞虱研究进展. 江西农业学报, 2012, 24(1): 91–97 Deng W, Hu L X, Chen H P, Chen M L, Xiao Y Q. Research progress in resistance of rice to Sogatella furcifera. Acta Agric Jiangxi, 2012, 24: 91–97 (in Chinese with English abstract) [21] Himabindu K, Suneetha K, Sama V S A K, Bentur J S. A new rice gall midge resistance gene in the breeding line CR57-MR1523, mapping with flanking markers and development of NILs. Euphytica, 2010, 174(2): 179–187 [22] Yang L, Zhang W L. Genetic and biochemical mechanisms of rice resistance to planthopper. Plant Cell Rep, 2016, 35: 1559–1572 [23] Tuyen L Q, Liu Y Q, Jiang L, Wang B X, Wang Q, Hanh T T T, Wan J M. Identification of quantitative trait loci associated with small brown planthopper (Laodelphax striatellusFallén) resistance in rice (Oryza sativa L.). Hereditas, 2012, 149: 16–23 [24] Wang Q, Liu Y Q, Hu J L, Zhang Y X, Xie K, Wang B X, Tuyen L Q, Song Z Q, Wu H, Liu Y L, Jiang L, Liu SJ, Cheng X N, Wang C M, Zhai H Q, Wan J M. Detection of quantitative trait loci (QTLs) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. Int J Mol Sci, 2013, 14: 8406–8421 [25] Zhang W L, Dong Y, Yang L, Ma B J, Ma R R, Huang F D, Wang C C, Hu H T, Li C S, Yan C Q, Chen J P. Small brown planthopper resistance loci in wild rice (Oryza officinalis). Mol Genet Genom, 2014, 289: 373–382

[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[4] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[5] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[6] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[7] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[8] 鲁雅妮, 丁超杰, 张煜, 杜习军, 齐学礼, 胡琳, 许为钢. 河南省200份小麦品种苗期茎基腐病抗性鉴定与全基因组关联分析[J]. 作物学报, 2026, 52(2): 363-375.
[9] 董丽华, 董成艳, 李正楠, 余静, 叶靓, 刘芳, 谭静. 玉米禾谷镰孢穗腐病抗性候选基因的筛选与鉴定[J]. 作物学报, 2026, 52(1): 131-147.
[10] 苏爱国, 肖森林, 易红梅, 段赛茹, 王帅帅, 张如养, 邢锦丰, 李春辉, 孙轩, 徐瑞斌, 徐田军, 李志勇, 张勇, 王荣焕, 宋伟, 赵久然. 玉米穗腐病的抗性遗传研究进展与育种应用[J]. 作物学报, 2026, 52(1): 1-13.
[11] 朱锦程, 杨秋华, 程李香, 李文丽, 石明明, 李惠霞, 张峰. 马铃薯抗南方根结线虫种质资源筛选及相关生理反应分析[J]. 作物学报, 2025, 51(9): 2307-2317.
[12] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[13] 高梦娟, 赵贺莹, 陈家辉, 陈晓倩, 牛萌康, 钱琪润, 崔陆飞, 邢江敏, 银庆淼, 郭雯, 张宁, 孙丛苇, 阳霞, 裴丹, 贾奥琳, 陈锋, 余晓东, 任妍. 小麦抗纹枯病新位点Qse.hnau-5AS的定位及其候选基因鉴定[J]. 作物学报, 2025, 51(8): 2240-2250.
[14] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
[15] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!