作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1596-1602.doi: 10.3724/SP.J.1006.2017.01596
仲杰1,温培正1,孙志广1,肖世卓1,胡金龙1,张乐1,江玲1,程遐年1,刘裕强1,*,万建民1,2
ZHONG Jie1,WEN Pei-Zheng1,SUN Zhi-Guang1,XIAO Shi-Zhuo1,HU Jin-Long1,ZHANG Le1,JIANG Ling1,CHENG Xia-Nian1,LIU Yu-Qiang1,*,WAN Jian-Min1,2
摘要:
灰飞虱是我国水稻生产的主要害虫之一,不仅直接取食危害水稻,还是水稻主要病毒病的传播介体,严重制约水稻生产。籼稻品种MR1523对灰飞虱表现较强的排趋性。为发掘抗灰飞虱新基因,本研究利用MR1523与感虫粳稻品种苏御糯构建了一个包含200个家系的F2:3分离群体,进行灰飞虱抗性鉴定。并利用120对均匀分布在水稻12条染色体的多态性SSR标记,构建了全基因组连锁图谱,进行抗灰飞虱QTL定位。结果分别在水稻第2、第5和第6染色体上检测到Qsbph2、Qsbph5a、Qsbph5b和Qsbph6 4个抗灰飞虱QTLs,分别位于分子标记RM526–RM3763、RM17804–RM13、RM574–RM169和RM190–RM510之间,LOD值分别为2.14、3.13、3.23和2.35,贡献率分别为12.0%、14.7%、17.4%和14.1%,各QTL的抗性等位基因效应均来自抗虫亲本MR1523。该结果为后续抗灰飞虱基因的精细定位及通过分子标记辅助选择培育抗灰飞虱水稻新品种奠定了基础。
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