烤烟6个农艺性状的QTL定位

doi:10.3724/SP.J.1006.2012.01407

摘要/Abstract

摘要： 由于烟草的分子标记开发和遗传图谱构建十分困难，迄今烟草中有关数量性状基因座(QTL)的定位研究仍非常有限。本研究利用一个由207个株系组成的烤烟DH群体及基于该群体所构建的含有24个连锁群、611个SSR标记，总长为1 882.1 cM的遗传图谱，采用复合区间作图方法，对株高(PH)、茎围(SG)、节距(IL)、叶片数(LN)、最大腰叶长(LWL)和最大腰叶宽(WWL) 6个与叶片产量有关的农艺性状进行QTL定位分析。共检测到69个QTL，大部分QTL的效应值较小，仅有4个具有较大的效应值，可解释大约15%~20%的表型变异。6个性状之间大多彼此相关。与此相符，在基因组中发现存在许多小区域，每个区域包含两个或两个以上紧密连锁的不同性状的QTL。

关键词: 烤烟, 数量性状基因座, 遗传作图, 加倍单倍体, 产量

Abstract: Studies of genetic mapping of quantitative trait loci (QTLs) in tobacco have still been very limited up to date due to the difficulty of molecular marker development and genetic map construction in this species. In this study, with a doubled haploid (DH) population of flue-cured tobacco and a genetic map consisting of 611 SSR markers on 24 linkage groups (LGs) and spanning a total length of 1 882.1 cM constructed based on this population, QTL mapping was performed using the method of composite interval mapping for six agronomic traits related to leaf yield, including plant height (PH), stem girth (SG), internode length (IL), leaf number (LN), length of the largest waist leaf (LWL) and width of the largest waist leaf (WWL). A total of 69 QTLs were detected. Most of the QTLs had small effects, but there were four with relatively large effects, explaining 15–20% of the phenotypic variation in the DH population. The six traits were largely correlated with each other. Consistent with this, many small regions harboring two or more closely linked QTLs of different traits were found in the genome.

Key words: Flue-cured tobacco (Nicotiana tabacum L.), Quantitative trait locus (QTL), Gene

童治军，焦芳婵，吴兴富，王丰青，陈学军，李绪英，高玉龙，张谊寒，肖炳光，吴为人. 烤烟6个农艺性状的QTL定位[J]. 作物学报, 2012, 38(08): 1407-1415.

TONG Zhi-Jun,JIAO Fang-Chan,WU Xing-Fu,WANG Feng-Qing,CHEN Xue-Jun,LI Xu-Ying,GAO Yu-Long,ZHANG Yi-Han,XIAO Bing-Guang,WU Wei-Ren. Mapping of Quantitative Trait Loci Underlying Six Agronomic Traits in Flue-Cured Tobacco (Nicotiana tabacum L.)[J]. Acta Agron Sin, 2012, 38(08): 1407-1415.

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