作物学报 ›› 2019, Vol. 45 ›› Issue (5): 792-797.doi: 10.3724/SP.J.1006.2019.84104
吴含玉1,3,肖飞1,张亚黎2,姜闯道3,*(),张旺锋2,*()
Han-Yu WU1,3,Fei XIAO1,Ya-Li ZHANG2,Chuang-Dao JIANG3,*(),Wang-Feng ZHANG2,*()
摘要:
除持续强光导致光合作用效率降低外, 强闪光也能够影响光合功能, 但规律和机制尚不清楚。为研究强闪光对喜光植物棉花叶片光合功能的影响, 选用陆地棉(Gossypium hirsutum L.)品种新陆早45号为材料, 于强闪光处理(20,000 μmol m -2 s -1, 300 ms, 间隔10 s, 处理时间持续30 min)前后分别测定叶绿素荧光、P700和气体交换。结果表明, 强闪光处理后不仅有活性的PSI (光系统I)反应中心含量下降, 同时PSII (光系统II)电子传递活性也受到限制。与对照相比, 强闪光处理后PSI的ΦND (PSI供体侧限制引起的非光化学量子产量)下降, ΦNA (PSI受体侧限制引起的非光化学量子产量)增加, 暗示强闪光能够抑制PSI受体侧电子传递活性。强闪光处理不仅使PSII的实际量子产量明显下降, 而且非光化学猝灭和ΦNPQ (PSII调节性能量耗散的量子产量)也降低; 但是, ΦNO (PSII非调节性能量耗散的量子产量)明显增加, 表明强闪光导致热耗散降低和PSII失活。此外, 强闪光处理后光合速率和气孔导度均降低, 但细胞间隙CO2浓度增加, 证明强闪光处理后同化能力的降低不是气孔限制导致的。因此, 本研究认为强闪光处理不仅抑制PSI活性, 而且导致PSII失活和可调节性热耗散下降; 光合电子传递活性的下降可能是强闪光下光合速率降低的重要原因。
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