作物学报 ›› 2020, Vol. 46 ›› Issue (10): 1496-1506.doi: 10.3724/SP.J.1006.2020.04028
刘荣1,**(
), 王芳1,**(
), 方俐1,**(
), 杨涛1, 张红岩1, 黄宇宁1, 王栋1,3, 季一山1, 徐东旭2, 李冠1, 郭瑞军1, 宗绪晓1,*(
)
LIU Rong1,**(
), WANG Fang1,**(
), FANG Li1,**(
), YANG Tao1, ZHANG Hong-Yan1, HUANG Yu-Ning1, WANG Dong1,3, JI Yi-Shan1, XU Dong-Xu2, LI Guan1, GUO Rui-Jun1, ZONG Xu-Xiao1,*(
)
摘要:
豌豆(Pisum sativum L.)是一种重要的食用豆类作物, 在全世界范围内广泛种植, 既可作为人类食物, 也可作为牲畜饲料。用SSR标记构建的遗传连锁图谱在豌豆和其他作物的标记辅助育种中发挥着重要的作用。尽管对豌豆遗传连锁作图的研究已有悠久历史, 但公众可获得且可转移的SSR标记以及基于遗传独特的中国豌豆种质的高密度遗传连锁图谱仍然有限。为了获得更多可转移的SSR标记和中国豌豆的高密度遗传连锁图谱, 本研究首先从自主开发和文献获取的12,491个全基因组SSR标记中筛选了617个多态性SSR标记, 并用于G0003973×G0005527 F2群体遗传连锁图谱的加密。加密后的图谱全长扩展到5330.6 cM, 包含603个SSR标记, 标记平均间距离8.8 cM, 相比之前的图谱有明显改善。基于上述结果, 我们又筛选了119个具有多态性的SSR标记, 用于构建大样本W6-22600×W6-15174 F2群体的遗传连锁图谱, 新图谱累积长度为1127.1 cM, 包含118个SSR标记, 装配在7条连锁群上。最后, 将来自以上2个遗传图谱的数据进行整合, 得到了一张覆盖范围6592.6 cM的整合图谱, 包含668个SSR标记, 由509个基因组SSR、134个EST-SSR和25个锚定标记组成, 分布在7条连锁群上。这些SSR标记和遗传连锁图谱将为豌豆的遗传研究和标记辅助育种提供有力工具。
| [1] |
Zong X X, Redden R J, Liu Q C, Wang S M, Guan J P, Liu J, Xu Y H, Liu X J, Gu J, Yan L, Ades P, Ford R. Analysis of a diverse global Pisum sp collection and comparison to a Chinese local P. sativum collection with microsatellite markers. Theor Appl Genet, 2009,118:193-204.
doi: 10.1007/s00122-008-0887-z |
| [2] |
Smýkal P, Aubert G, Burstin J, Coyne C J, Ellis N T H, Flavell A J, Ford R, Hýbl M, Macas J, Neumann P, McPhee K E, Redden R J, Rubiales D, Weller J L, Warkentin T D. Pea (Pisum sativum L.) in the genomic era. Agronomy, 2012,2:74-115.
doi: 10.3390/agronomy2020074 |
| [3] |
Tian S J, Kyle W S A, Small D M. Pilot scale isolation of proteins from field peas (Pisum sativum L.) for use as food ingredients. Int J Food Sci Technol, 1999,34:33-39.
doi: 10.1046/j.1365-2621.1999.00236.x |
| [4] |
Santalla M, Amurrio J M, De Ron A M. Food and feed potential breeding value of green, dry and vegetable pea germplasm. Can J Plant Sci, 2001,81:601-610.
doi: 10.4141/P00-114 |
| [5] | FAOSTAT. [2020-03-14]. http://www.fao.org/faostat/en/#data. |
| [6] |
MacWilliam S, Wismer M, Kulshreshtha S. Life cycle and economic assessment of Western Canadian pulse systems: The inclusion of pulses in crop rotations. Agric Syst, 2014,123:43-53.
doi: 10.1016/j.agsy.2013.08.009 |
| [7] | Semagn K, Bjornstad A, Ndjiondjop M N. Principles, requirements and prospects of genetic mapping in plants. Afr J Biotechnol, 2006,5:2569-2587. |
| [8] |
Dirlewanger E, Isaac P G, Ranade S, Belajouza M, Cousin R, de Vienne D. Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theor Appl Genet, 1994,88:17-27.
doi: 10.1007/BF00222388 pmid: 24185876 |
| [9] |
Laucou V, Haurogne K, Ellis N, Rameau C. Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum. Theor Appl Genet, 1998,97:905-915.
doi: 10.1007/s001220050971 |
| [10] |
Loridon K, McPhee K, Morin J, Dubreuil P, Pilet Nayel M L, Aubert G, Rameau C, Baranger A, Coyne C, Lejeune Henaut I, Burstin J. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor Appl Genet, 2005,111:1022-1031.
doi: 10.1007/s00122-005-0014-3 |
| [11] |
DeCaire J, Coyne C J, Brumett S, Shultz J L. Additional pea EST-SSR markers for comparative mapping in pea (Pisum sativum L.). Plant Breed, 2012,131:222-226.
doi: 10.1111/pbr.2011.131.issue-1 |
| [12] |
Mishra R K, Gangadhar B H, Nookaraju A, Kumar S, Park S W. Development of EST-derived SSR markers in pea (Pisum sativum) and their potential utility for genetic mapping and transferability. Plant Breed, 2012,131:118-124.
doi: 10.1111/pbr.2011.131.issue-1 |
| [13] |
Leonforte A, Sudheesh S, Cogan N O I, Salisbury P A, Nicolas M E, Materne M, Forster J W, Kaur S. SNP marker discovery, linkage map construction and identification of QTLs for enhanced salinity tolerance in field pea (Pisum sativum L.). BMC Plant Biol, 2013,13:161.
doi: 10.1186/1471-2229-13-161 pmid: 24134188 |
| [14] |
Sindhu A, Ramsay L, Sanderson L A, Stonehouse R, Li R, Condie J, Shunmugam A S K, Liu Y, Jha A B, Diapari M, Burstin J, Aubert G, Tar’an B, Bett K E, Warkentin T D, Sharpe A G. Gene-based SNP discovery and genetic mapping in pea. Theor Appl Genet, 2014,127:2225-2241.
doi: 10.1007/s00122-014-2375-y |
| [15] |
Sun X L, Yang T, Hao J J, Zhang X Y, Ford R, Jiang J Y, Wang F, Guan J P, Zong X X. SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties. Crop J, 2014,2:170-174.
doi: 10.1016/j.cj.2014.03.004 |
| [16] |
Tayeh N, Aluome C, Falque M, Jacquin F, Klein A, Chauveau A, Berard A, Houtin H, Rond C, Kreplak J, Boucherot K, Martin C, Baranger A, Pilet-Nayel M L, Warkentin T D, Brunel D, Marget P, Le Paslier M C, Aubert G, Burstin J. Development of two major resources for pea genomics: the GenoPea 13.2K SNP Array and a high-density, high-resolution consensus genetic map. Plant J, 2015,84:1257-1273.
doi: 10.1111/tpj.13070 pmid: 26590015 |
| [17] |
Boutet G, Carvalho S A, Falque M, Peterlongo P, Lhuillier E, Bouchez O, Lavaud C, Pilet-Nayel M L, Riviere N, Baranger A. SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population. BMC Genomics, 2016,17:121.
doi: 10.1186/s12864-016-2447-2 pmid: 26892170 |
| [18] |
Ma Y, Coyne C J, Grusak M A, Mazourek M, Cheng P, Main D, McGee R J. Genome-wide SNP identification, linkage map construction and QTL mapping for seed mineral concentrations and contents in pea (Pisum sativum L.). BMC Plant Biol, 2017,17:43.
doi: 10.1186/s12870-016-0956-4 pmid: 28193168 |
| [19] |
Barilli E, Cobos M J, Carrillo E, Kilian A, Carling J, Rubiales D. A high-density integrated DArTseq SNP-based genetic map of Pisum fulvum and identification of QTLs controlling rust resistance. Front Plant Sci, 2018,9:167.
doi: 10.3389/fpls.2018.00167 pmid: 29497430 |
| [20] |
Kreplak J, Madoui M A, Capal P, Novak P, Labadie K, Aubert G, Bayer P E, Gali K K, Syme R A, Main D, Klein A, Berard A, Vrbova I, Fournier C, d’Agata L, Belser C, Berrabah W, Toegelova H, Milec Z, Vrana J, Lee H, Kougbeadjo A, Terezol M, Huneau C, Turo C J, Mohellibi N, Neumann P, Falque M, Gallardo K, McGee R, Tar’an B, Bendahmane A, Aury J M, Batley J, Le Paslier M C, Ellis N, Warkentin T D, Coyne C J, Salse J, Edwards D, Lichtenzveig J, Macas J, Dolezel J, Wincker P, Burstin J. A reference genome for pea provides insight into legume genome evolution. Nat Genet, 2019,51:1411-1422.
doi: 10.1038/s41588-019-0480-1 pmid: 31477930 |
| [21] |
Kalia R K, Rai M K, Kalia S, Singh R, Dhawan A K. Microsatellite markers: an overview of the recent progress in plants. Euphytica, 2011,177:309-334.
doi: 10.1007/s10681-010-0286-9 |
| [22] |
Vieira M L C, Santini L, Diniz A L, Munhoz C D. Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol, 2016,39:312-328.
doi: 10.1590/1678-4685-GMB-2016-0027 pmid: 27561112 |
| [23] |
Ali A, Pan Y B, Wang Q N, Wang J D, Chen J L, Gao S J. Genetic diversity and population structure analysis of Saccharum and Erianthus genera using microsatellite (SSR) markers. Sci Rep, 2019,9:10.
doi: 10.1038/s41598-018-36877-0 pmid: 30626881 |
| [24] |
Hao L, Zhang G S, Lu D Y, Hu J J, Jia H X. Analysis of the genetic diversity and population structure of Salix psammophila based on phenotypic traits and simple sequence repeat markers. PeerJ, 2019,7:e6419.
doi: 10.7717/peerj.6419 pmid: 30805247 |
| [25] |
Choi J K, Sa K J, Park D H, Lim S E, Ryu S H, Park J Y, Park K J, Rhee H I, Lee M, Lee J K. Construction of genetic linkage map and identification of QTLs related to agronomic traits in DH population of maize (Zea mays L.) using SSR markers. Genes Genomics, 2019,41:667-678.
doi: 10.1007/s13258-019-00813-x pmid: 30953340 |
| [26] |
Yang T, Jiang J Y, Zhang H Y, Liu R, Strelkov S, Hwang S F, Chang K F, Yang F, Miao Y M, He Y H, Zong X X. Density enhancement of a faba bean genetic linkage map (Vicia faba) based on simple sequence repeats markers. Plant Breed, 2019,138:207-215.
doi: 10.1111/pbr.2019.138.issue-2 |
| [27] |
Anjani K, Ponukumatla B, Mishra D, Ravulapalli D P. Identification of simple-sequence-repeat markers linked to Fusarium wilt (Fusarium oxysporum f. sp carthami) resistance and marker- assisted selection for wilt resistance in safflower (Carthamus tinctorius L.) interspecific offsprings. Plant Breed, 2018,137:895-902.
doi: 10.1111/pbr.2018.137.issue-6 |
| [28] |
Swathi G, Rani C V D, Md J, Madhav M S, Vanisree S, Anuradha C, Kumar N R, Kumar N A P, Kumari K A, Bhogadhi C, Ramprasad E, Sravanthi P, Raju S K, Bhuvaneswari V, Rajan C P D, Jagadeeswar R. Marker-assisted introgression of the major bacterial blight resistance genes, Xa21 and xa13, and blast resistance gene, Pi54, into the popular rice variety, JGL1798. Mol Breed, 2019,39:12.
doi: 10.1007/s11032-018-0919-6 |
| [29] |
Kumar N, Shikha D, Kumari S, Choudhary B K, Kumar L, Singh I S. SSR-based DNA Fingerprinting and diversity assessment among Indian germplasm of Euryale ferox: an aquatic underutilized and neglected food crop. Appl Biochem Biotechnol, 2018,185:34-41.
doi: 10.1007/s12010-017-2643-9 pmid: 29082475 |
| [30] |
Siew G Y, Ng W L, Tan S W, Alitheen N B, Tan S G, Yeap S K. Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers. PeerJ, 2018,6:e4266.
doi: 10.7717/peerj.4266 pmid: 29511604 |
| [31] |
Tayeh N, Aubert G, Pilet Nayel M L, Lejeune Henaut I, Warkentin T D, Burstin J. Genomic tools in pea breeding programs: Status and perspectives. Front Plant Sci, 2015,6:1037.
doi: 10.3389/fpls.2015.01037 pmid: 26640470 |
| [32] |
Liu R, Fang L, Yang T, Zhang X Y, Hu J G, Zhang H Y, Han W L, Hua Z K, Hao J J, Zong X X. Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections. Sci Rep, 2017,7:5919.
doi: 10.1038/s41598-017-06222-y pmid: 28724947 |
| [33] |
Wu X B, Li N N, Hao J J, Hu J G, Zhang X Y, Blair M W. Genetic diversity of Chinese and global pea (Pisum sativum L.) collections. Crop Sci, 2017,57:1-11.
doi: 10.2135/cropsci2015.07.0415 |
| [34] |
Milczarski P, Bolibok Brągoszewska H, Myśków B, Stojałowski S, Heller Uszyńska K, Góralska M, Brągoszewski P, Uszyński G, Kilian A, Rakoczy Trojanowska M. A high density consensus map of rye (Secale cereale L.) based on DArT markers. PLoS One, 2011,6:e28495.
doi: 10.1371/journal.pone.0028495 pmid: 22163026 |
| [35] |
Blenda A, Fang D D, Rami J F, Garsmeur O, Luo F, Lacape J M. A high density consensus genetic map of tetraploid cotton that integrates multiple component maps through molecular marker redundancy check. PLoS One, 2012,7:e45739.
doi: 10.1371/journal.pone.0045739 pmid: 23029214 |
| [36] |
Sudheesh S, Lombardi M, Leonforte A, Cogan N O I, Materne M, Forster J W, Kaur S. Consensus genetic map construction for field pea (Pisum sativum L.), trait dissection of biotic and abiotic stress tolerance and development of a diagnostic marker for the er1 powdery mildew resistance gene. Plant Mol Biol Rep, 2015,33:1391-1403.
doi: 10.1007/s11105-014-0837-7 |
| [37] |
Sudheesh S, Rodda M, Kennedy P, Verma P, Leonforte A, Cogan N O I, Materne M, Forster J W, Kaur S. Construction of an integrated linkage map and trait dissection for bacterial blight resistance in field pea (Pisum sativum L.). Mol Breed, 2015,35:185.
doi: 10.1007/s11032-015-0376-4 |
| [38] |
Yang T, Fang L, Zhang X Y, Hu J G, Bao S Y, Hao J J, Li L, He Y H, Jiang J Y, Wang F, Tian S, Zong X X. High-throughput development of SSR markers from pea (Pisum sativum L.) based on next generation sequencing of a purified Chinese commercial variety. PLoS One, 2015,10:e0139775.
doi: 10.1371/journal.pone.0139775 pmid: 26440522 |
| [39] |
Kwon S J, Brown A F, Hu J, McGee R, Watt C, Kisha T, Timmerman-Vaughan G, Grusak M, McPhee K E, Coyne C J. Genetic diversity, population structure and genome-wide marker-trait association analysis emphasizing seed nutrients of the USDA pea (Pisum sativum L.) core collection. Genes Genomics, 2012,34:305-320.
doi: 10.1007/s13258-011-0213-z |
| [40] |
Kaur S J, Pembleton L W, Cogan N O, Savin K W, Leonforte T, Paull J, Materne M, Forster J W. Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers. BMC Genomics, 2012,13:104.
doi: 10.1186/1471-2164-13-104 pmid: 22433453 |
| [41] |
Xu S C, Gong Y M, Mao W H, Hu Q Z, Zhang G W, Fu W, Xian Q Q. Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosae). Am J Bot, 2012,99:E149-E153.
doi: 10.3732/ajb.1100445 |
| [42] | Bordat A, Savois V, Nicolas M, Salse J, Chauveau A, Bourgeois M, Potier J, Houtin H, Rond C, Murat F, Marget P, Aubert G, Burstin J. Translational genomics in legumes allowed placing in silico 5460 unigenes on the pea functional map and identified candidate genes in Pisum sativum L. G3: Genes Genom Genet 2011,1:93-103. |
| [43] | 顾竟, 李玲, 宗绪晓, 王海飞, 关建平, 杨涛. 豌豆种质表型性状SSR标记关联分析. 植物遗传资源学报, 2011,12:833-839. |
| Gu J, Li L, Zong X X, Wang H F, Guan J P, Yang T. Association analysis between morphological traits of pea and its polymorphic SSR markers. J Plant Genet Resour, 2011,12:833-839 (in Chinese with English abstract). | |
| [44] |
Burstin J, Deniot G, Potier J, Weinachter C, Aubert G, Barranger A. Microsatellite polymorphism in Pisum sativum. Plant Breed, 2001,120:311.
doi: 10.1046/j.1439-0523.2001.00608.x |
| [45] |
Dellaporta S L, Wood J, Hicks J B. A plant DNA minipreparation: version II. Plant Mol Biol Rep, 1983,1:19-21.
doi: 10.1007/BF02712670 |
| [46] |
Meng L, Li H H, Zhang L Y, Wang J K. QTL IciMapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015,3:269-283.
doi: 10.1016/j.cj.2015.01.001 |
| [47] |
Voorrips R E. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered, 2002,93:77-78.
doi: 10.1093/jhered/93.1.77 pmid: 12011185 |
| [48] |
Parida S K, Kalia S K, Kaul S, Dalal V, Hemaprabha G, Selvi A, Pandit A, Singh A, Gaikwad K, Sharma T R, Srivastava P S, Singh N K, Mohapatra T. Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theor Appl Genet, 2009,118:327-338.
doi: 10.1007/s00122-008-0902-4 |
| [49] | Kamaluddin , Khan M A, Kiran U, Ali A, Abdin M Z, Zargar M Y, Ahmad S, Sofi P A, Gulzar S. Molecular markers and marker-assisted selection in crop plants. In: Abdin M Z, Kiran U, Kamaluddin, Ali A, eds. Plant Biotechnology: Principles and Applications. Singapore: Springer Singapore, 2017. pp 295-328. |
| [50] |
Nadeem M A, Nawaz M A, Shahid M Q, Doğan Y, Comertpay G, Yıldız M, Hatipoğlu R, Ahmad F, Alsaleh A, Labhane N, Özkan H, Chung G, Baloch F S. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnol Biotechnol Equip, 2018,32:261-285.
doi: 10.1080/13102818.2017.1400401 |
| [51] |
Varshney R K, Graner A, Sorrells M E. Genic microsatellite markers in plants: features and applications. Trends Biotechnol, 2005,23:48-55.
doi: 10.1016/j.tibtech.2004.11.005 pmid: 15629858 |
| [52] |
Smýkal P, Hybl M, Corander J, Jarkovsky J, Flavell A J, Griga M. Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theor Appl Genet, 2008,117:413-424.
doi: 10.1007/s00122-008-0785-4 |
| [53] |
宗绪晓, 关建平, 王述民, 刘庆昌. 中国豌豆地方品种SSR标记遗传多样性分析. 作物学报, 2008,34:1330-1338.
doi: 10.3724/SP.J.1006.2008.01330 |
| Zong X X, Guan J P, Wang S M, Liu Q C. Genetic diversity among Chinese pea (Pisum sativum L.) landraces revealed by SSR markers. Acta Agron Sin, 2008,34:1330-1338 (in Chinese with English abstract). | |
| [54] |
Zong X X, Ford R, Redden R R, Guan J P, Wang S M. Identification and analysis of genetic diversity structure within Pisum genus based on microsatellite markers. Agric Sci China, 2009,8:257-267.
doi: 10.1016/S1671-2927(08)60208-4 |
| [55] |
宗绪晓, 关建平, 王述民, 刘庆昌, Redden R R, Ford R. 国外栽培豌豆遗传多样性分析及核心种质构建. 作物学报, 2008,34:1518-1528.
doi: 10.3724/SP.J.1006.2008.01518 |
| Zong X X, Guan J P, Wang S M, Liu Q C, Redden R R, Ford R. Genetic diversity and core collection of alien Pisum sativum L. germplasm. Acta Agron Sin, 2008,34:1518-1528 (in Chinese with English abstract). | |
| [56] | Prakash N, Kumar R, Choudhary V K, Singh C M. Molecular assessment of genetic divergence in pea genotypes using microsatellite markers. Legume Res, 2016,39:183-188. |
| [57] |
Duarte J, Riviere N, Baranger A, Aubert G, Burstin J, Cornet L, Lavaud C, Lejeune Henaut I, Martinant J P, Pichon J P, Pilet Nayel M L, Boutet G. Transcriptome sequencing for high throughput SNP development and genetic mapping in pea. BMC Genom, 2014,15:126.
doi: 10.1186/1471-2164-15-126 |
| [58] |
Guindon M F, Martin E, Cravero V, Gali K K, Warkentin T D, Cointry E. Linkage map development by GBS, SSR, and SRAP techniques and yield-related QTLs in pea. Mol Breed, 2019,39:54.
doi: 10.1007/s11032-019-0949-8 |
| [59] |
Aubert G, Morin J, Jacquin F, Loridon K, Quillet M C, Petit A, Rameau C, Lejeune Henaut I, Huguet T, Burstin J. Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula. Theor Appl Genet, 2006,112:1024-1041.
doi: 10.1007/s00122-005-0205-y |
| [60] |
Duarte J, Riviere N, Baranger A, Aubert G, Burstin J, Cornet L, Lavaud C, Lejeune Henaut I, Martinant J P, Pichon J P, Pilet Nayel M L, Boutet G. Transcriptome sequencing for high throughput SNP development and genetic mapping in pea. BMC Genom, 2014,15:126.
doi: 10.1186/1471-2164-15-126 |
| [61] |
Sindhu A, Ramsay L, Sanderson L A, Stonehouse R, Li R, Condie J, Shunmugam A S K, Liu Y, Jha A B, Diapari M, Burstin J, Aubert G, Tar’an B, Bett K E, Warkentin T D, Sharpe A G. Gene-based SNP discovery and genetic mapping in pea. Theor Appl Genet, 2014,127:2225-2241.
doi: 10.1007/s00122-014-2375-y |
| [62] |
Sybenga J. Recombination and chiasmata: few but intriguing discrepancies. Genome, 1996,39:473-484.
doi: 10.1139/g96-061 pmid: 18469909 |
| [63] |
Knox M R, Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations. Genetics, 2002,162:861-873.
pmid: 12399396 |
| [64] | Truong S K, McCormick R F, Morishige D T, Mullet J E. Resolution of genetic map expansion caused by excess heterozygosity in plant recombinant inbred populations. G3: Genes Genom Genet, 2014,4:1963-1969. |
| [65] |
Ellis T H, Turner L, Hellens R P, Lee D, Harker C L, Enard C, Domoney C, Davies D R. Linkage maps in pea. Genetics, 1992,130:649-663.
pmid: 1551583 |
| [1] | 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603. |
| [2] | 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400. |
| [3] | 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072. |
| [4] | 邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735. |
| [5] | 闫尚龙, 王琦明, 柴强, 殷文, 樊志龙, 胡发龙, 刘志鹏, 韦金贵. 绿洲灌区玉米籽粒产量及品质对密植及间作豌豆的响应[J]. 作物学报, 2025, 51(6): 1665-1675. |
| [6] | 周恩强, 缪亚梅, 周瑶, 姚梦楠, 赵娜, 王永强, 朱宇翔, 薛冬, 李宗迪, 石宇欣, 李波, 汪凯华, 顾春燕, 王学军, 魏利斌. 基于种子发育转录组的豌豆bZIP基因家族分析及种子发育候选基因的鉴定[J]. 作物学报, 2025, 51(4): 914-931. |
| [7] | 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885. |
| [8] | 郑栋, 周仙莉, 滕长才, 侯万伟, 张红岩, 刘玉皎. 基于SSR标记的青海蚕豆品种亲缘关系分析与指纹图谱构建[J]. 作物学报, 2025, 51(1): 79-90. |
| [9] | 匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116. |
| [10] | 黄淑贤, 刘荣, 李冠, 疏琴, 徐斐, 宗绪晓, 杨涛. 通过CRISPR/Cas9建立豌豆基因组大片段敲除体系[J]. 作物学报, 2024, 50(7): 1658-1668. |
| [11] | 丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Dipak K SANTRA, 王瑞云, 陈喜明. 东北春播区糜子核心种质及其DNA分子身份证构建[J]. 作物学报, 2024, 50(5): 1181-1192. |
| [12] | 田春艳, 边芯, 郎荣斌, 俞华先, 桃联安, 安汝东, 董立华, 张钰, 经艳芬. 甘蔗3个育种性状与SSR标记的关联分析及优异等位变异发掘[J]. 作物学报, 2024, 50(2): 310-324. |
| [13] | 陈天, 李昱樱, 荣二花, 吴玉香. 棉属人工异源四倍体后代性状鉴定及花器转录组学分析[J]. 作物学报, 2024, 50(2): 325-339. |
| [14] | 陈志凯, 周仙莉, 张红岩, 滕长才, 侯万伟. 320份蚕豆蛋白质含量的SSR关联分析[J]. 作物学报, 2024, 50(11): 2775-2786. |
| [15] | 曹越, 张立媛, 辛旭霞, 冯智尊, 郭娟, 王晓丹, 曹晓宁, SANTRA Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR的宁夏糜子DNA分子身份证的构建[J]. 作物学报, 2024, 50(11): 2699-2711. |
|
||