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作物学报 ›› 2021, Vol. 47 ›› Issue (12): 2394-2406.doi: 10.3724/SP.J.1006.2021.04259

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜CBF基因家族的鉴定和表达分析

解盼1,4(), 刘蔚1, 康郁1, 华玮1, 钱论文1,2,3, 官春云1,2,3,*, 何昕1,2,3,*   

  1. 1湖南农业大学南方粮油作物协同创新中心, 湖南长沙 410128
    2湖南农业大学油料作物研究所, 湖南长沙 410128
    3国家油料作物改良中心湖南分中心, 湖南长沙 410128
    4湖南农业大学风景园林与艺术设计学院, 湖南长沙 410128
  • 收稿日期:2020-11-28 接受日期:2021-04-14 出版日期:2021-12-12 网络出版日期:2021-05-21
  • 通讯作者: 官春云,何昕
  • 作者简介:E-mail: xiepan@hunau.edu.cn
  • 基金资助:
    湖南省科技创新计划项目(2020RC2057);国家自然科学基金项目(31801398)

Identification and relative expression analysis of CBF gene family in Brassica napus L.

XIE Pan1,4(), LIU Wei1, KANG Yu1, HUA Wei1, QIAN Lun-Wen1,2,3, GUAN Chun-Yun1,2,3,*, HE Xin1,2,3,*   

  1. 1Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha 410128, Hunan, China
    2Oil Crops Research, Hunan Agricultural University, Changsha 410128, Hunan, China
    3Hunan Branch of National Oilseed Crops Improvement Center, Changsha 410128, Hunan, China
    4College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, Hunan, China
  • Received:2020-11-28 Accepted:2021-04-14 Published:2021-12-12 Published online:2021-05-21
  • Contact: GUAN Chun-Yun,HE Xin
  • Supported by:
    Science and Technology Innovation Program of Hunan Province(2020RC2057);National Natural Science Foundation of China(31801398)

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摘要：

低温是影响植物生长发育的重要环境胁迫因子, ICE1 (inducer of CBF expression1)-CBF (C-repeat (CRT)-binding factors)-COR (cold responsive)在植物响应低温胁迫的信号途径中具有重要作用。为探究CBF (C-repeat-binding factors)基因在甘蓝型油菜(Brassica napus L.)中的进化以及在低温胁迫应答反应中的功能, 本研究以4个拟南芥CBF基因为基础序列, 鉴定出11个甘蓝型油菜、6个白菜和5个甘蓝CBF基因, 并对它们的分子特性、蛋白保守结构域和系统进化树、基因结构及基因染色体分布、甘蓝型油菜CBF基因组织表达模式以及不同逆境和激素处理下的表达模式进行系统的比较分析。结果表明, 在11个甘蓝型油菜CBF基因中, 可分为亚组I (CBF1/2/3)和亚组II (CBF4) 2个亚组。转录组测序结果表明, 所有甘蓝型油菜CBF基因受低温诱导表达, 其中亚组Ib的4个基因对冷胁迫响应迅速且持续时间长, 亚组II中2个基因对冷胁迫响应表达较弱, 但它们在根中表达量明显高于叶片, 并且参与盐胁迫和渗透胁迫响应; 甘蓝型油菜中CBF基因家族对冻害响应更强烈, 其中亚组I中的BnaA08g30930D、BnaCnng49280D、BnaAnng34260D、BnaC07g39680D和亚组II中BnaA10g07630D、BnaC09g28190D对冻害响应尤为显著。本研究将为进一步了解甘蓝型油菜CBF家族基因的生物学功能及其对低温尤其是冻害响应奠定基础, 同时为CBF家族基因在其他物种中的生物信息学研究提供参考。

关键词: 甘蓝型油菜, CBF, 低温, 冻害, 基因表达分析

Abstract:

Low temperature is an important environmental stress factor affecting plant growth and development. The ICE1-CBF-COR [inducer of CBF expression1-c-repeat (CRT)-binding factors-cold responsive] plays an important role in the signaling pathway in response to low temperature stress in plants. To explore the evolution of CBF gene in Brassica napus L. and its function in response to low temperature stress, 11, 6, and 6 CBF genes were detected from Brassica napus, Brassica rapa, and Brassica oleracea, respectively. In addition, the molecular characteristics, protein conserved domains, phylogenetic tree, gene structure, and chromosome distribution were systematically investigated. The results showed that 11 BnaCBF genes could be divided into two subgroups including Subgroup I (CBF1/2/3) and Subgroup II (CBF4). Transcriptome sequencing revealed that all the CBF genes of Brassica napus were induced by low temperature, among which four genes in subgroup Ib responded rapidly and durably to cold stress, and two genes in Subgroup II responded weakly to cold stress, however, the relative expression level of CBF genes in roots was significantly higher than that in leaves, which were involved in salt stress and osmotic stress responses. Further analysis demonstrated that the CBF gene family in Brassica napus responded more strongly to freezing stress, especially BnaA08G30930D, BnaCnng49280D, BnaAnng34260D, BnaC07g39680D in Subsection I, and BnaA10g07630D, BnaC09G28190D in Subsection II. This study lays a foundation for further understanding the biological function of the CBF family gene and its response to low temperature, especially freezing stress in Brassica napus., and provides a reference for bioinformation research of CBF family genes in other species.

Key words: Brassica napus L., CBF, low temperature, chilling stress, relative expression level

表1

本研究所用qRT-PCR引物"

基因名称
Gene name		 正向引物序列
Forward sequence (5°-3°)		 负向引物序列
Reverse sequence (5°-3°)
BnaCBF4-RT GAGACCACGGAAGAGAACGG AGTCGTTATGATTCCATCCAAGTT
BnaCBF1-C09-RT CGACTATGGTTGAGGCAGTGAAA AAGTCGTTATGATTCCATCCAAGTT
BnaCBF3-RT CCAAGCCGAGTCAGCAAAAT GAGAAACTCAGGTAAATGGGTGTG
BnaCBF1-Cnn-RT GAGACGACGGTGGAGGGTG AAGTCATCATTATGTCCCCATTGTA
BnaC05g34300D/BnaACTIN GGTTGGGATGGACCAGAAGG TCAGGAGCAATACGGAGC

表2

BnaCBF基因信息汇总"

基因名称
Gene ID		 核苷酸长度Nucleotide length (bp) 氨基酸长度
Amino
Acid
length		 等电点预测
Isoelectric point prediction		 分子量Molecular weight (kD) 内含子数目
Number of introns		 外显子数目Number of exons 亚细胞定位
Subcellular localization		 染色体定位
Chromosome location		 白菜同源基因Homologous gene in Brassica rape 甘蓝同源基因Homologous gene in Brassica oleracea
BnaA08g30950D 666 221 4.97 24.56 2 3 核酸Nuclear chrA08_random:1781546..1782397 Bra010460 Bo8g050800
BnaUnng01150D 651 216 5.34 23.81 1 3 核酸Nuclear chrUnn_random:1448150..1448983
BnaA08g30910D 654 217 5.13 24.02 2 3 核酸Nuclear chrA08_random:1768075..1768896 Bra010461
BnaA08g30930D 570 189 4.73 20.57 2 3 核酸Nuclear chrA08_random:1771443..1772198 Bra010463 Bo8g050740
BnaCnng49280D 576 191 4.58 20.83 2 3 核酸Nuclear chrCnn_random:48753744..48754496
BnaA03g13620D 645 214 4.58 20.82 0 1 核酸Nuclear chrA03:6228484..6229128 Bra022770 Bo3g024240
BnaC03g71900D 645 214 5.03 23.79 0 1 核酸Nuclear chrC03_random:417520..418164
BnaAnng34260D 645 214 5.21 23.83 0 1 核酸Nuclear chrAnn_random:39020794..39021438 Bra019162 Bo7g110320
BnaC07g39680D 648 215 5.23 23.81 0 1 核酸Nuclear chrC07:40421468..40422115
BnaA10g07630D 663 220 5.52 24.55 0 1 核酸Nuclear chrA10:6107845..6108507 Bra028290 Bo9g104770
BnaC09g28190D 663 220 6.14 24.5 0 1 核酸Nuclear chrC09:30491719..30492381

图1

甘蓝型油菜、白菜、甘蓝和拟南芥CBF序列比对分析 黑色: 26条序列的共有碱基; 红色: 18~25条序列的共有碱基; 蓝色: 9~17条序列的共有碱基; 白色: 1~8条序列的共有碱基。"

图2

拟南芥、白菜、甘蓝、甘蓝型油菜CBF的系统比较分析 A: 系统发育分析; B: 基因结构分析; C: 保守结构MEME分析。"

图3

拟南芥、白菜、甘蓝、甘蓝型油菜染色体上CBF基因的分布 染色体编号标记在每个染色体的左侧。Chr是拟南芥染色体; A和C分别是白菜和甘蓝的染色体; chrA和chrC分别是甘蓝型油菜的A亚组和C亚组染色体; Random表示该基因不确定在此条染色体的具体位置, chrAnn和chrCnn分别表示属于A亚基因组和C亚基因组但不确定染色体信息, chrUnn表示不确定亚基因组及染色体信息。"

图4

BnaCBF基因在不同组织器官中的表达热图 每个样本中每个基因的表达水平(FPKM值)由不同颜色的矩形表示。红色表示高表达; 蓝色表示低表达。"

图5

不同非生物胁迫和植物激素处理下BnaCBF基因的表达热图 每个样本中每个基因的表达水平(FPKM值)由不同颜色的矩形表示。红色表示高表达; 蓝色表示低表达。Leaf_3h、Leaf_6h、Leaf_12h: 正常生长3 h、6 h、12 h的叶片; Cold_3h、Cold_6h、Cold_12h: 4℃处理3 h、6 h、12 h的叶片; Hot_3h、Hot_6h、Hot_12h: 40℃处理3 h、6 h、12 h的叶片; ABA_3h、ABA_6h、ABA_12h: 脱落酸处理3 h、6 h、12 h的叶片; MeJA_3h、MeJA_6h、MeJA_12h: 茉莉酸甲酯处理3 h、6 h、12 h的叶片; ET_3h、ET_6h、ET_12h: 乙烯处理3 h、6 h、12 h的叶片;SA_3h、SA_6h、SA_12h: 盐胁迫处理3 h、6 h、12 h的叶片; Root_3h、Root_6h、Root_12h: 正常生长3 h、6 h、12 h的根;NaCl_3h、NaCl_6h、NaCl_12h: 氯化钠处理3 h、6 h、12 h的根; PEG_3h、PEG_6h、PEG_12h: 聚乙二醇处理3 h、6 h、12 h的根。"

图6

不同低温处理下BnaCBF基因表达热图的研究 HX17_MA: 正常生长条件下的HX17材料; HX17_CA: 驯化14 d后4℃处理的HX17材料; HX17_FA: 驯化14 d后-4℃处理HX17材料。HX17_MB: 正常生长条件下HX17材料; HX17_CB: 4℃处理HX17材料; HX17_FB: -4℃处理HX17材料。HX58_MA: 正常生长条件下的HX58材料; HX58_CA: 驯化14天后4℃处理的HX58材料; HX58_FA: 驯化14 d后-4℃处理HX58材料。HX58_MB: 正常生长条件下HX58材料; HX58_CB: 4℃处理HX58材料; HX58_FB: -4℃处理HX58材料。"

图7

低温胁迫与茉莉酸甲酯处理下BnaA10g07630D和BnaC09g28190D基因表达模式的qRT-PCR分析 处理同图5。"

图8

低温胁迫处理下BnaCBF基因表达模式的qRT-PCR分析 17_MA: 正常生长条件下的HX17材料; 17_CA: 驯化14 d后4℃处理的HX17材料; 17_FA: 驯化14 d后-4℃处理HX17材料。17_MB: 正常生长条件下HX17材料; 17_CB: 4℃处理HX17材料; 17_FB: -4℃处理HX17材料。"

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