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作物学报 ›› 2021, Vol. 47 ›› Issue (2): 332-341.doi: 10.3724/SP.J.1006.2021.04106
雷永1(
), 王志慧1, 淮东欣1, 高华援2, 晏立英1, 李建国1, 李威涛1, 陈玉宁1, 康彦平1, 刘海龙2, 王欣1, 薛晓梦1, 姜慧芳1, 廖伯寿1,*()
LEI Yong1(), WANG Zhi-Hui1, HUAI Dong-Xin1, GAO Hua-Yuan2, YAN Li-Ying1, LI Jian-Guo1, LI Wei-Tao1, CHEN Yu-Ning1, KANG Yan-Ping1, LIU Hai-Long2, WANG Xin1, XUE Xiao-Meng1, JIANG Hui-Fang1, LIAO Bo-Shou1,*()
摘要：
含糖量是决定和影响花生食用品质和加工特性的重要指标, 蔗糖含量占成熟花生籽仁总糖量的90%以上, 建立蔗糖含量的高效检测技术, 有助于加快高蔗糖甜味食用型花生品种培育进程。本研究利用蔗糖含量差异显著的185份花生材料, 利用近红外仪(波长范围1100~2500 nm), 配合小样品杯, 扫描和采集自然干燥籽仁的近红外光谱, 采用液相色谱(HPLC)结合标准曲线法测定试验材料的蔗糖含量, 利用偏最小二乘法(partial least squares, PLS)构建了花生籽仁蔗糖含量的近红外定标模型, 模型的决定系数R2 = 0.962, 均方差为0.383。利用20份材料对模型进行外部验证, 预测值和化学值的决定系数达0.947, 表明该模型可较好地预测蔗糖含量, 可以高效地测定杂交早期世代的单株花生蔗糖含量。利用该模型在“吉花02-1-4×中花26”杂交后代中发掘出6份含糖量7%以上、油酸78%以上、含油量48%以下, 且农艺性状优良的食用花生新品系。
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