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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (9): 1691-1697.doi: 10.3724/SP.J.1006.2009.01691

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Plant Growth Substances on Morphological and Anatomical Structure of Leaf and Photosynthetic Characteristics in Soybean

FENG Nai-Jie1,ZHENG Dian-Feng1,*,ZHAO Jiu-Xiang1,ZU Wei2,DU Ji-Dao1,ZHANG Yu-Xian1,LIANG Xi-Long1   

  1. 1 Crop Growth and Development Regulation Laboratory, College of Agronomy, Heilongjiang August First Land Reclamation University, Daqing 163319, China; 2 College of Agronomy, Northeast Agricultural University, Harbin 150031, China
  • Received:2008-12-24 Revised:2009-04-16 Online:2009-09-12 Published:2009-07-04
  • Contact: ZHENG Dian-Feng, E-mail: zdffnj@263.net; Tel: 0459-6819175

Abstract:

Many soybean [Glycine max (L.) Merr.] morphological and physiological regulations responding to plant growth substances have been reported. Related researches showed that plant growth substances could effectively control lodging, increase leaf area index, promote photosynthesis, reduce productive abscission and improve yield and quality in soybean production. Plant growth substances thorough adjusting inner hormone control soybean’s whole process of growth and development, at the same time possibly affect the structure of soybean organs, including root, stem, leaf, and productive organs, on which there are a little report. Microtechnique and ultramicrotechnique have triumphantly applied in the researches on the changes of fibre texture and ultrastructure in plant caused by nutrient elements and environment factors, which provides availibity to research the effects of plant growth substances on the structure of soybean organs. The present study was carried out in an attempt to compare anatomical structure and photosynthetic characteristics of cultivar Kennong 4, Treated with three plant growth substances In the field of experiments on trail farm of Heilongjiang August-First Land Reclamation University in 2006. The selected secure plant growth substances Diethyl anlinoethyl (DTA), Ckolirte chloride (Cc), and SOD simulation material (SODM), were applied by leaf-spraying at the beginning of blossom stage (R1). 30 days later, at the beginning seed stage (R5), the functional leaves were sampled to compare fibre texture and ultrastructure by microtechnique and ultramicrotechnique. Photosynthetic characteristics, such as leaf index, photosynthetic pigment content, photosynthetic potential and photosynthetic rate, were measured every 10 days after the treatments.The results indicated that the plant growth substances caused the changes of both anatomical structure of leaf and photosynthetic characteristics. Under light microscope, we found that, compared with control, DTA, CC, and SODM increased palisade tissue thickness and ratio of palisade/spongy of leaf. The tight degree of palisade tissue arrangement,was different in treatment showing the order of SODM> DTA> CC> CK. DTA, CC, and SODM increased the number of chloroplast single cell, granule lamella and starch grains in chloroplast, while reduced the number of osmophilic globuli in the chloroplast. SODM, DTA increased granule number in chloroplast significantly. In the investigation of photosynthetic characteristics, we found that, compared with control, DTA, SODM, and CC increased the contents of Chl a, Chl b, Chl(a+b) in leaf and the ratio of Chl b/a. DTA, SODM, and CC advanced the time to maximum leaf area index and leaf area duration (LAD), increased total LAD and photosynthetic rate during grain filling period under water stress. From above, we drew a conclusion that plant growth substances could at least partially regulate the structure of soybean organs, fibre texture and ultrastructure of soybean leaf, which is helpful to promote photosynthesis, showing the consistency between anatomical structure and physiological function.

Key words: Soybean, Plant growth substances, Morphological and anatomical, Photosynthetic characteristic

[1] Davies D D. The Biochemistry of Plants. New York: Academic Press Inc, 1980. pp 86-96

[2] Hatch M D, Boardman N K. The Biochemistry of Plants. New York: Academic Press Tnc, 1981. pp 37-46

[3] Hall D O, Rao K K. Photosynthesis, 4th edn. London: Edwa Atnold Limited, 1988. pp 15-21

[4] Salisbury F B, Ross C W. Plant Physiology. California: California Wadsworth Publishing Company Inc. 1991. pp 87-111

[5]Pan R-Z(潘瑞炽). Plant Physiology (植物生理学). Beijing: Higher Education Press,1995. pp 87-117 (in Chinese)

[6] Li P-Q(李培庆), Chen S-K(陈善坤).Studies on effects of PP-(333) on the dynamic change of C-N metabolism in soybean at different development stages and it s relation to the yield formation of soybean. Acta Agric Univ Jiangxiensis (江西农业大学学报), 1992, 14(4): 366-370(in Chinese with English abstract)

[7] Wang M(王敏), Yao W-C(姚维传), Zhang C-Y(张从宇). Effects of plant growth regulator on seedling growth under drought stress in soybean.J Soil Water Conserv (水土保持学报), 2005, 19(4): 190-193(in Chinese with English abstract)

[8] Zhang M-C(张明才), He Z-P(何钟佩), Tian X-L(田晓莉), Duan L-S(段留生), Wang B-M(王保民), Zhai Z-X(翟志席), Dong X-H(董学会), Li Z-H(李召虎). Effects of plant growth regulator SHK-6 on physiological function of soybean leaves under water deficiency.Acta Agron Sin (作物学报), 2005, 31(9): 1215-1220 (in Chinese with English abstract)

[9] Yu H-Q(于海秋), Peng X-X(彭新湘), Yan X-L(严小龙), Cao M-J(曹敏建). Effect of phosphorus deficiency on microstructure and photosynthesis in soybean leaves. J Jilin Agric Univ (吉林农业大学学报), 2006, 28(2): 127-132 (in Chinese with English abstract)

[10] Zhang G-R(张桂茹), Du W-G(杜维广), Man W-Q(满为群), Li G-Q(李桂芹), Gui M-Z(桂明珠), Wang X-D(王学东), Ge Q-Y(戈巧英), Hao N-B(郝乃斌). Study on leaf comparative anatomy of different genotypes of soybean. Chin Bull Bot (植物学通报), 2002, 19(2): 208-214 (in Chinese with English abstract)

[11] Yu Y(于颖), Liu Y-Y(刘元英), Luo S-G(罗盛国), Peng X-L(彭显龙). Effects of selenium on soybean chloroplast ultra-structure and microelement content of soybean leaves under continuous cropping stress. Chin J Appl Ecol (应用生态学报), 2003, 14(4): 573-576 (in Chinese with English abstract)

[12] Liu J(刘杰), Liu L-J(刘丽君), Wu J-J(吴俊江), Chen Y-L(陈伊里). Progress and prospect of research on soybean ultrastructures. Soybean Sci (大豆科学), 2004, 23(3): 228-231 (in Chinese with English abstract)

[13] Tian X-L(田晓莉), Tan W-M(谭伟明), Li Z-H(李召虎), Wang B-M(王保民), He Z-P(何钟佩), Duan L-S(段留生). The effects of mixture of DPC and DTA-6 on seedlings of insect-resistant transgenic cotton. Cotton Sci (棉花学报), 2006, 18(1): 3-7 (in Chinese with English abstract)

[14] Lin J-H(林加涵), Wei W-L(魏文铃), Peng X-X(彭宣宪). Modern BiologyExperiments (现代生物学实验). Higher Education Press, 2002. pp 70-82 (in Chinese)

[15] Wang X-D(王学东), Cang J(苍晶), Zhang P(张鹏), Cui L(崔琳). Observation of soybean funiculus of transfer tissue.J Chin Electron Microscopy Soc (电子显微学报), 2004, 23(4): 354-354 (in Chinese)

[16] Zhao H-J(赵会杰), Zou Q(邹琦), Zhang X-Y(张秀英). Comparison between two wheat varieties with different spike type in carbohydrate metabolism during late growth period. Acta Agron Sin (作物学报), 2002, 28(5): 654-659 (in Chinese with English abstract)

[17] Zhang X-Z(张宪政). Research Method for Crop Physiology (作物生理研究法). Beijing: Agriculture Press, 1992. p 68 (in Chinese)

[18] Chaves M M. Effects of water deficits on carbon assimilation. J Exp Bot, 1991, 42: 1-16

[19] Ma G-Y(马国英), Wu Y-Y(吴源英), Xu X-Z(徐锡忠). Comparative studies on leaf blade photosynthetic characteristic and chloroplast ultramicrostructure of different wheat cultivars. Plant Physiol Commun (植物生理学通讯), 1991, 27(4): 255-259 (in Chinese with English abstract)

[20] Fan Y-P(范燕萍), Li H-L(李慧玲), Li H-J(李浩健). Pigment composition and ultrastructural difference of chloroplast in three kinds of variegation leaf of cymbidium sinense. J South China Agric Univ (华南农业大学学报), 2006, 27(2): 8-12 (in Chinese with English abstract)

[21] Grover A, Mohanty P. Leaf Senescence-induced alterations in structure and function of higher plant chloroplasts. In: Abrol Y P, Mohany P, eds. Photosynthesis: Photoreactions to Plant Productivity. Dordrecht: Kluwer Academic Publishers, 1992. pp 225-255

[22] Tong Z-Y(童朝阳), Han L-M(韩丽梅), Zou Y-J(邹永久). Effect of soybean Specificy complex fertilizer of ultrastructre of chloroplast under different cropping systems. Soybean Sci (大豆科学), 1998, 17(4): 369-371 (in Chinese with English abstract)

[23]Zhang H-S(张恒善), Cheng Y-X(程砚喜), Wang D-Q(王大秋), Xiang S-H(项淑华), Gao M(高敏), Wang X-F(王雪飞), Niu J-G(牛建光).Correlation analysis of variation in chlorophyll content and yield in pod period of soybean. Soybean Sci (大豆科学), 2001, 20(4): 276-278(in Chinese with English abstract)

[24] Demming-Adams B, Adams W W III. The role of xanthophylls cycle carotenoids in the protenction of photosynthesis. Trends Plant Sci, 1996, 1: 21-26

[25] Liu Z-Q(刘贞琦), Liu Z-Y(刘振业), Ma D-P(马达鹏), Zeng S-F(曾淑芬).A study on the relation between chlorophyll content and photosynthetic rate of rice. Acta Agron Sin (作物学报), 1984, 10(1): 57-62 (in Chinese with English abstract)

[26] Dong Z(董钻). Soybean Yield Physiology (大豆产量生理). Beijing: China Agriculture Press, 1999. pp 138-141 (in Chinese)
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