欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (10): 1696-1703.doi: 10.3724/SP.J.1006.2008.01696

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用回交导入系定位干旱环境下水稻植株水分状况相关QTL

赵秀琴1;徐建龙1;朱苓华1;黎志康1,2,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程, 北京100081; 2 International Rice Research In-stitute, DAPO Box 7777, Metro Manila, Philippines
  • 收稿日期:2008-01-28 修回日期:1900-01-01 出版日期:2008-10-12 网络出版日期:2008-10-12
  • 通讯作者: 黎志康

Locating QTLs for Plant Water Status under Drought Condition in Over-lapping Introgression Lines of Rice (Oryza sativa L.)

ZHAO Xiu-Qin1,XU Jian-Long1,ZHU Ling-Hua1,LI Zhi-Kang12*   

  1. 1 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
  • Received:2008-01-28 Revised:1900-01-01 Published:2008-10-12 Published online:2008-10-12
  • Contact: LI Zhi-Kang

PDF

1563

被引次数

9

摘要： 日益严重的水资源危机使水稻的耐旱育种尤为迫切, 干旱环境下较高的植株水分含量有助于提高或维持作物产量的稳定性, 挖掘与耐旱性密切相关的分子标记有助于提高耐旱品种的选育效率。从供体Lemont (粳稻)导入到特青(籼稻)背景的254个高代回交导入系中筛选出覆盖供体全基因组的55个回交导入系, 采用PVC管栽培, 分析了干旱(胁迫)条件下水稻植株水分状况相关性状与籽粒产量、生物量的相关性并定位了相关QTL。研究表明, 植株水分相关性状(相对含水量、叶片水势、渗透势、卷叶度)均与籽粒产量显著相关。检测到7个相对含水量QTL, 7个叶片水势QTL, 5个渗透势QTL及5个卷叶QTL; 另检测到5个产量QTL, 7个生物量QTL。分析发现, 不仅QLwp5、QLr5、QRwc5和QY5同时分布在RM509~RM163区域, 且该区域还分布有对水分环境表现稳定的产量QTL(QGy5), 效应方向一致, 从遗传学角度解释了籽粒产量与水分相关性状之间的显著相关性。另外, QLr5、QRwc5、QY5、QLr2、QLr7、QLr8、QLr9、QRwc3、QRwc4a、QRwc12及QY7 等11个QTL曾在不同遗传背景群体中被检测到, 它们控制相同目标性状。研究认为RM509~RM163区域及QLr2、QLr7、QLr8、QLr9、QRwc3、QRwc4a、QRwc12和QY7所分布的染色体区域对水分环境或者遗传背景相对稳定, 在水稻分子标记辅助选择(MAS)耐旱育种实践中有较重要利用价值。

关键词: 水稻, 干旱, 植株水分状况, 回交导入系, 数量性状位点

Abstract: Drought tolerance (DT) is becoming one of the most important target traits in rice variety improvement under ever-increasing severe drought situation all over the world and identifying genomic regions contributing to DT will help to develop cultivars suitable to drought environments. The maintenance of higher plant water status are known to contribute to increase yield and yield stability under drought in cereals. In present study, the relationship between the traits related to plant water status and grain yield (GY) was analyzed and the quantitative trait loci (QTLs) linked to target traits were mapped by means of 55 introgression lines (ILs) selected from 254 advanced backcross introgression population derived from Lemont/Teqing in the Teqing background. The ILs and parents were planted in PVC pipes in screen house and phenotyped under drought and/or irrigation conditions. Significant phenotypic correlation between GY and the traits related to plant water status was observed. QTLs related to plant water status were identified which included seven for relative water content (RWC), seven for leaf water potential (LWP), five for osmotic potential (Y) and five for leaf rolling (LR) under drought condition, moreover, five QTLs for grain yield (GY) and five QTLs for biomass (BM) were detected under both drought and control conditions. By comparing the coincidence of QTLs with specific traits, we genetically dissected the nature of association among different traits related to plant water status and the association between GY and plant water status under drought. This study demonstrated that the region RM509–RM163 on chromosome 5 identified for plant water status-related DT component QTLs also had pleiotropic effects on yield under stress and control conditions. Moreover, the eleven QTLs, QLr5, QRwc5, QY5, QLr2, QLr7, QLr8, QLr9, QRwc3, QRwc4a, QRwc12, and QY7 controlling the same traits were detected in different genetic populations which means these regions were relatively stable to the environment or/and genetic backgrounds. The consistent QTLs for plant water status under stress might be useful for marker as-sisted selection (MAS) breeding in rice for DT improvement.

Key words: Rice, Drought, Plant water status, Backcross introgression lines, Quantitative trait locus

[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[4] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[5] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[6] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[7] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[8] 张胜忠, 李国卫, 戈立江, 王菲菲, 胡晓辉, 苗华荣, 李燕, 钟文, 陈静. 花生机械脱壳损伤相关农艺指标筛选与QTL定位[J]. 作物学报, 2026, 52(2): 644-652.
[9] 杨飚, 杜帅康, 张继旺, 石瑛, 张丽莉. 马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析[J]. 作物学报, 2026, 52(2): 405-420.
[10] 刘海波, 张蕾, 王立琦, 石晓丽, 周文莹, 崔国贤, 佘玮. 苎麻BnGCL1基因响应干旱胁迫的功能研究[J]. 作物学报, 2026, 52(1): 14-27.
[11] 姬炫彤, 卞春松, 金黎平, 李森, 秦军红, 李广存. 不同耐旱型马铃薯根际微生物对干旱的响应[J]. 作物学报, 2026, 52(1): 165-177.
[12] 胡城祯, 高维东, 孔斌雪, 王建飞, 车卓, 杨德龙, 陈涛. 小麦TaAPC11基因家族鉴定及TaAPC11-5B参与干旱胁迫的生物学功能研究[J]. 作物学报, 2026, 52(1): 148-164.
[13] 王雅致, 杨飚, 季香林, 石瑛, 张丽莉. 二倍体马铃薯抗旱资源鉴定及抗旱基因初步筛选[J]. 作物学报, 2026, 52(1): 72-84.
[14] 孔娜, 刘涛, 刘文婷, 陈刚, 文利超, 邓智超, 郭梅, 李伟, 郭永峰. 烟草NtCEP7基因克隆及其编码小肽在苗期抗旱中的作用分析[J]. 作物学报, 2026, 52(1): 249-261.
[15] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2